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Title | Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED. |
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Journal, issue, pages | Nat Methods, Vol. 14, Issue 4, Page 399-402, Year 2017 |
Publish date | Feb 13, 2017 |
![]() | M Jason de la Cruz / Johan Hattne / Dan Shi / Paul Seidler / Jose Rodriguez / Francis E Reyes / Michael R Sawaya / Duilio Cascio / Simon C Weiss / Sun Kyung Kim / Cynthia S Hinck / Andrew P Hinck / Guillermo Calero / David Eisenberg / Tamir Gonen / ![]() |
PubMed Abstract | Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from ...Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography. |
![]() | ![]() ![]() ![]() |
Methods | EM (electron crystallography) |
Resolution | 1.1 - 2.9 Å |
Structure data | EMDB-8216, PDB-5k7n: EMDB-8217, PDB-5k7o: EMDB-8218, PDB-5k7p: EMDB-8219, PDB-5k7q: EMDB-8220, PDB-5k7r: EMDB-8221, PDB-5k7s: |
Chemicals | ![]() ChemComp-HOH: ![]() ChemComp-CL: ![]() ChemComp-NA: ![]() ChemComp-IOD: ![]() ChemComp-CA: ![]() ChemComp-ZN: ![]() ChemComp-DMS: ![]() ChemComp-IPA: |
Source |
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![]() | PROTEIN FIBRIL / Amyloid / HYDROLASE / PLANT PROTEIN / Sweet protein / TRANSFERASE |