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Structure paper

TitleAtomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
Journal, issue, pagesNat Methods, Vol. 14, Issue 4, Page 399-402, Year 2017
Publish dateFeb 13, 2017
AuthorsM Jason de la Cruz / Johan Hattne / Dan Shi / Paul Seidler / Jose Rodriguez / Francis E Reyes / Michael R Sawaya / Duilio Cascio / Simon C Weiss / Sun Kyung Kim / Cynthia S Hinck / Andrew P Hinck / Guillermo Calero / David Eisenberg / Tamir Gonen /
PubMed AbstractTraditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from ...Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
External linksNat Methods / PubMed:28192420 / PubMed Central
MethodsEM (electron crystallography)
Resolution1.1 - 2.9 Å
Structure data

8216

5k7n

EMDB-8216, PDB-5k7n:
MicroED structure of tau VQIVYK peptide at 1.1 A resolution
Method: EM (electron crystallography) / Resolution: 1.1 Å

8217

5k7o

EMDB-8217, PDB-5k7o:
MicroED structure of lysozyme at 1.8 A resolution
Method: EM (electron crystallography) / Resolution: 1.8 Å

8218

5k7p

EMDB-8218, PDB-5k7p:
MicroED structure of xylanase at 2.3 A resolution
Method: EM (electron crystallography) / Resolution: 2.3 Å

8219

5k7q

EMDB-8219, PDB-5k7q:
MicroED structure of thaumatin at 2.5 A resolution
Method: EM (electron crystallography) / Resolution: 2.5 Å

8220

5k7r

EMDB-8220, PDB-5k7r:
MicroED structure of trypsin at 1.7 A resolution
Method: EM (electron crystallography) / Resolution: 1.7 Å

8221

5k7s

EMDB-8221, PDB-5k7s:
MicroED structure of proteinase K at 1.6 A resolution
Method: EM (electron crystallography) / Resolution: 1.6 Å

8222

5k7t

EMDB-8222, PDB-5k7t:
MicroED structure of thermolysin at 2.5 A resolution
Method: EM (electron crystallography) / Resolution: 2.5 Å

8472

5ty4

EMDB-8472, PDB-5ty4:
MicroED structure of a complex between monomeric TGF-b and its receptor, TbRII, at 2.9 A resolution
Method: EM (electron crystallography) / Resolution: 2.9 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-IOD:
IODIDE ION

ChemComp-CA:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

Source
  • homo sapiens (human)
  • gallus gallus (chicken)
  • Chicken (chicken)
  • Trichoderma reesei (fungus)
  • hypocrea jecorina (fungus)
  • thaumatococcus daniellii (katemfe)
  • bos taurus (cattle)
  • engyodontium album (fungus)
  • bacillus thermoproteolyticus (bacteria)
KeywordsPROTEIN FIBRIL / Amyloid / HYDROLASE / PLANT PROTEIN / Sweet protein / TRANSFERASE

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