+検索条件
-Structure paper
| タイトル | Reducing effects of particle adsorption to the air-water interface in cryo-EM. |
|---|---|
| ジャーナル・号・ページ | Nat Methods, Vol. 15, Issue 10, Page 793-795, Year 2018 |
| 掲載日 | 2018年9月24日 |
著者 | Alex J Noble / Hui Wei / Venkata P Dandey / Zhening Zhang / Yong Zi Tan / Clinton S Potter / Bridget Carragher / ![]() |
| PubMed 要旨 | Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt ...Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin. |
リンク | Nat Methods / PubMed:30250056 / PubMed Central |
| 手法 | EM (トモグラフィー) / EM (単粒子) |
| 解像度 | 3.77 - 6.09 Å |
| 構造データ | ![]() EMDB-7623: ![]() EMDB-7624: ![]() EMDB-7625: ![]() EMDB-7627: ![]() EMDB-7628: ![]() EMDB-7629: ![]() EMDB-7630: ![]() EMDB-7788: ![]() EMDB-7791: ![]() EMDB-7792: |
| 由来 |
|
ムービー
コントローラー
構造ビューア
万見文献について



著者
リンク









Tabanus atratus (昆虫)
Homo sapiens (ヒト)
Influenza A virus (A/Hong Kong/26/1983(H3N2)) (A型インフルエンザウイルス)