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-Structure paper
Title | Reducing effects of particle adsorption to the air-water interface in cryo-EM. |
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Journal, issue, pages | Nat Methods, Vol. 15, Issue 10, Page 793-795, Year 2018 |
Publish date | Sep 24, 2018 |
![]() | Alex J Noble / Hui Wei / Venkata P Dandey / Zhening Zhang / Yong Zi Tan / Clinton S Potter / Bridget Carragher / ![]() |
PubMed Abstract | Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt ...Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air-water or substrate-water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin. |
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Methods | EM (tomography) / EM (single particle) |
Resolution | 3.77 - 6.09 Å |
Structure data | ![]() EMDB-7623: ![]() EMDB-7624: ![]() EMDB-7625: ![]() EMDB-7627: ![]() EMDB-7628: ![]() EMDB-7629: ![]() EMDB-7630: ![]() EMDB-7788: ![]() EMDB-7791: ![]() EMDB-7792: |
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