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TitleClamp conformational flexibility and dynamics in archaeal and eukaryotic RNA polymerases revealed by cryo-EM
Journal, issue, pagesTo Be Published
Publish dateDec 15, 2025 (structure data deposition date)
AuthorsFordjour GNR / Palao III L / Murakami K / Armache J-P / Murakami KS
External linksSearch PubMed
MethodsEM (single particle)
Resolution3.31 - 8.9 Å
Structure data

74465

9zo4

EMDB-74465, PDB-9zo4:
Cryo-EM structure of the complete Pyrococcus furiosus RNA polymerase in closed clamp conformation
Method: EM (single particle) / Resolution: 4.05 Å

74466

9zo5

EMDB-74466, PDB-9zo5:
Cryo-EM structure of the complete Pyrococcus furiosus RNA polymerase in open clamp conformation
Method: EM (single particle) / Resolution: 4.05 Å

74490

9zof

EMDB-74490, PDB-9zof:
Cryo-EM structure of the complete Sulfolobus acidocaldarius RNA polymerase in closed clamp conformation
Method: EM (single particle) / Resolution: 3.37 Å

74496

9zoh

EMDB-74496, PDB-9zoh:
Cryo-EM structure of the complete Sulfolobus acidocaldarius RNA polymerase in open clamp conformation
Method: EM (single particle) / Resolution: 3.87 Å

74528

9zpk

EMDB-74528, PDB-9zpk:
Cryo-EM structure of the complete Saccharomyces cerevisiae RNA polymerase II in closed clamp conformation
Method: EM (single particle) / Resolution: 3.31 Å

74529

9zpl

EMDB-74529, PDB-9zpl:
Cryo-EM structure of the complete Saccharomyces cerevisiae RNA polymerase II in open clamp conformation
Method: EM (single particle) / Resolution: 3.75 Å

77809

36ro

EMDB-77809, PDB-36ro:
Cryo-EM structure of Pyrococcus furiosus RNA polymerase without stalk (no Rpo4/Rpo7)
Method: EM (single particle) / Resolution: 4.56 Å

77810

36rp

EMDB-77810, PDB-36rp:
Cryo-EM structure of Sulfolobus acidocaldarius RNA polymerase without stalk (no Rpo4/Rpo7)
Method: EM (single particle) / Resolution: 8.9 Å

77811

36rs

EMDB-77811, PDB-36rs:
Cryo-EM structure of Saccharomyces cerevisiae RNA polymerase II without stalk (no Rpb4/Rpb7)
Method: EM (single particle) / Resolution: 6.75 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

Source
  • pyrococcus furiosus dsm 3638 (archaea)
  • sulfolobus acidocaldarius dsm 639 (acidophilic)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSCRIPTION / RNA polymerase / Clamp conformation / Euryarchaea / Stalk / Crenarchaea / Eukaryote / Conformations / Promoter loading / RNA polymerase II

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