EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the transcriptional co-activator SAGA complex, including the histone acetyltransferase module.
ジャーナル・号・ページ	Mol Cell, Vol. 85, Issue 23, Page 4333-44346.e4, Year 2025
掲載日	2025年12月4日
著者	Rayees U H Mattoo / Dong-Hua Chen / David A Bushnell / Sagi Tamir / Roger D Kornberg /
PubMed 要旨	The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four ...The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four modules: transcription-associated protein 1 (Tra1), core, deubiquitination (DUB), and histone acetyltransferase (HAT). Although the structures of the Tra1, core, and DUB modules have been determined, the overall architecture of the HAT module remained elusive due to its inherent flexibility. To address this, we conducted cryo-electron microscopy (cryo-EM) analyses on SAGA purified from the thermophilic fungus Chaetomium thermophilum, yielding structures of Tra1 and core modules at 2.6 Å and three of the four HAT subunits at 3.7 Å. The structure of the HAT module was informative about the aspects of histone acetylation and the interface of HAT-core modules, contradicting earlier AlphaFold predictions. Our structure-guided genetic and biochemical analyses confirmed the roles of Ada1 and Spt7 in anchoring the HAT module within the SAGA complex.
リンク	Mol Cell / PubMed:41260211 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 4.4 Å
構造データ	73233 9ynu EMDB-73233, PDB-9ynu: Tra1 and core modules including core tip of ctSAGA complex 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-73234: Tra1 and Core Module of ctSAGA 手法: EM (単粒子) / 解像度: 2.7 Å 73235 9ynv EMDB-73235, PDB-9ynv: Histone Acetyl Transferase (HAT) module of ctSAGA 手法: EM (単粒子) / 解像度: 3.7 Å 73236 9ynw EMDB-73236, PDB-9ynw: Tra1, Core and minimal HAT modules of ctSAGA, composite. 手法: EM (単粒子) / 解像度: 3.5 Å 73237 9yzy EMDB-73237: Focused map of HAT module Bromodomain region PDB-9yzy: ctSAGA Focused on HAT Bromodomain region 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-73238: Focused map of Tra1 module of ctSAGA 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-73239: Focused map of Tra1 module of ctSAGA 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-73240: Focused map of Tra1 module of ctSAGA complex 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-73241: Focused map of core module of ctSAGA complex 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-73242: Focused map of core module of ctSAGA complex 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-73243: Focused map of core module of ctSAGA complex 手法: EM (単粒子) / 解像度: 2.9 Å 73261 9yol EMDB-73261, PDB-9yol: Tra1 module of ctSAGA 手法: EM (単粒子) / 解像度: 2.6 Å 73269 9yoq EMDB-73269, PDB-9yoq: Core module of ctSAGA 手法: EM (単粒子) / 解像度: 2.6 Å
由来	thermochaetoides thermophila (菌類)
キーワード	TRANSCRIPTION / ctSAGA complex / Histone Acetyl Transferase (HAT) Module / Tra1 module and Core module