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TitleRibosomal Architecture and rRNA Modification Landscape in the Tick-Borne Parasite .
Journal, issue, pagesbioRxiv, Year 2025
Publish dateNov 11, 2025
AuthorsCristina Gutierrez-Vargas / Lee S Izhaki-Tavor / Diana Calvopina-Chavez / Caroline D Keroack / Pablo Copello / Manoj T Duraisingh / Mélissa Léger-Abraham /
PubMed Abstract is a tick-borne intracellular apicomplexan parasite responsible for diseases ranging from mild to fatal, with a broadening geographic distribution. Due to the complex life cycle of species, their ... is a tick-borne intracellular apicomplexan parasite responsible for diseases ranging from mild to fatal, with a broadening geographic distribution. Due to the complex life cycle of species, their survival depends on the precise control of gene expression, which is primarily regulated by epigenetic, transcriptional, and post-transcriptional mechanisms. High-resolution structural information on key components of the translation machinery, such as ribosomes, could aid in the development of antiparasitic drugs. Here, we report cryo-EM ribosome structures (2.6 Å) from the tick-borne apicomplexan pathogen , showing associated tRNAs, an mRNA fragment, and RACK1, a signaling scaffold crucial to translation regulation. Density map analysis displays ribosome regions at atomic resolution (1.7 Å), which, when combined with nanopore sequencing, enabled the comprehensive identification of rRNA modifications, including modifications unreported in other organisms. The new rRNA modifications localize not only to the reduced rRNA expansion segments but also to functionally essential ribosomal sites, uncovering new avenues for therapeutic intervention against babesiosis.
External linksbioRxiv / PubMed:41293005 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 2.7 Å
Structure data

EMDB-72052: Consensus map for Babesia divergens ribosome structure by single-particle cryo-EM (3D class3, E-site tRNA)
Method: EM (single particle) / Resolution: 2.6 Å

EMDB-72055: Multi-body LSU map for Babesia divergens ribosome structure by single-particle cryo-EM (3D class3, E-site tRNA)
Method: EM (single particle) / Resolution: 2.5 Å

EMDB-72069: Multi-body SSU body map for Babesia divergens ribosome structure by single-particle cryo-EM (3D class3, E-site tRNA)
Method: EM (single particle) / Resolution: 2.6 Å

EMDB-72070: Multi-body SSU head map for Babesia divergens ribosome structure by single-particle cryo-EM (3D class3, E-site tRNA)
Method: EM (single particle) / Resolution: 2.7 Å

Source
  • Babesia divergens (eukaryote)

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