EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular mechanism of calcium permeability and magnesium block in NMDA receptors.
ジャーナル・号・ページ	Nat Neurosci, Vol. 29, Issue 6, Page 1293-1302, Year 2026
掲載日	2026年5月5日
著者	Ruben Steigerwald / Max Epstein / Tsung-Han Chou / Noriko Simorowski / Hiro Furukawa /
PubMed 要旨	Hebbian neuroplasticity, which is thought to be a cellular substrate of learning and memory, can occur by means of coincidental detection of presynaptic neurotransmitter release and Ca influx upon ...Hebbian neuroplasticity, which is thought to be a cellular substrate of learning and memory, can occur by means of coincidental detection of presynaptic neurotransmitter release and Ca influx upon postsynaptic depolarization. This is mediated at a molecular level by N-methyl-D-aspartate-type glutamate receptors, which bind glutamate and glycine and facilitate Ca influx upon relief of Mg channel block during membrane depolarization. However, the structural mechanism underlying Ca permeability and Mg blockade in N-methyl-D-aspartate-type glutamate receptors has yet to be fully elucidated. Here we demonstrate using single-particle cryo-electron microscopy that Ca permeation through the narrow constriction of the cation selectivity filter involves partial dehydration, as evidenced by several Ca binding sites. In contrast, Mg binds outside of the selectivity filter through a water network and remains hydrated, thereby acting as a channel blocker. Furthermore, the lipid network around the selectivity filter influences the stability of Mg binding in a voltage-dependent manner. Our study details the transmembrane chemistry essential for initiating neuroplasticity.
リンク	Nat Neurosci / PubMed:42086762 / PubMed Central
手法	EM (単粒子)
解像度	2.59 - 3.6 Å
構造データ	70297 9obs EMDB-70297, PDB-9obs: glutamate/glycine-bound GluN1a/2B NMDAR 手法: EM (単粒子) / 解像度: 3.18 Å 70298 9obt EMDB-70298, PDB-9obt: TMD of glutamate/glycine-bound GluN1a/2B NMDAR 手法: EM (単粒子) / 解像度: 3.01 Å 70299 9obu EMDB-70299, PDB-9obu: glycine/glutamate and Mg2+-bound GluN1a/2B NMDAR 手法: EM (単粒子) / 解像度: 3.16 Å 70300 9obv EMDB-70300, PDB-9obv: Mg2+-bound GluN1a/2B NMDAR (upper) 手法: EM (単粒子) / 解像度: 3.21 Å 70301 9obw EMDB-70301, PDB-9obw: Mg2+ bound GluN1a/2B NMDAR (lower) 手法: EM (単粒子) / 解像度: 3.15 Å 70302 9obx EMDB-70302, PDB-9obx: glutamate/glycine and Ca2+-bound GluN1a/2B NMDAR 手法: EM (単粒子) / 解像度: 2.59 Å 70303 9oby EMDB-70303, PDB-9oby: Ca2+-bound GluN1a/2B NMDAR (S1) 手法: EM (単粒子) / 解像度: 3.6 Å 70304 9obz EMDB-70304, PDB-9obz: Ca2+-bound GluN1a/2B NMDAR (S2) 手法: EM (単粒子) / 解像度: 2.81 Å 70305 9oc0 EMDB-70305, PDB-9oc0: Ca2+-bound GluN1a/2B NMDAR (S3) 手法: EM (単粒子) / 解像度: 2.97 Å 70306 9oc1 EMDB-70306, PDB-9oc1: Ca2+-bound GluN1a/2B NMDAR (S4) 手法: EM (単粒子) / 解像度: 2.76 Å 70307 9oc2 EMDB-70307, PDB-9oc2: Ca2+-bound GluN1a/2B NMDAR (S5) 手法: EM (単粒子) / 解像度: 2.69 Å
化合物	ChemComp-GLY: GLYCINE ChemComp-GLU: GLUTAMIC ACID ChemComp-CLR: CHOLESTEROL ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質YM ChemComp-HOH: WATER ChemComp-MG: Unknown entry ChemComp-CA*: Unknown entry
由来	rattus norvegicus (ドブネズミ)
キーワード	MEMBRANE PROTEIN / Ion Channels / NMDAR / membrane proteins / ion channel