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Structure paper

TitleMolecular mechanism of the catalysis for U12-type splicing by the human
Journal, issue, pagesVita, Year 2026
Publish dateNov 23, 2025 (structure data deposition date)
AuthorsBai R / Guo H / Sun R / Zhu Y / Shi Y / Wan R
External linksVita / Search PubMed
MethodsEM (single particle)
Resolution2.92 - 4.41 Å
Structure data

67250

9xtt

EMDB-67250, PDB-9xtt:
Human minor spliceosome branching-completed C complex (after step-I)
Method: EM (single particle) / Resolution: 2.92 Å

67263

9xu3

EMDB-67263, PDB-9xu3:
Human minor spliceosome exon-ligation-ready C* complex (prior to step-II)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-81104: Human minor spliceosome branching-completed C complex (after step-I) with stably-bound RBM48 region
Method: EM (single particle) / Resolution: 3.07 Å

EMDB-81123: Focused refinement map of SYF region from the human minor spliceosome branching-completed C complex
Method: EM (single particle) / Resolution: 3.21 Å

EMDB-81125: Focused refinement map of PRP16 region from the human minor spliceosome branching-completed C complex
Method: EM (single particle) / Resolution: 3.29 Å

EMDB-81127: Focused refinement map of helicase region (encompassing PRP16 and BRR2) from the human minor spliceosome branching-completed C complex
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-81129: Human minor spliceosome branching-completed C complex (after step-I) with stably-bound PRP8-RNase H region
Method: EM (single particle) / Resolution: 3.48 Å

EMDB-81130: Focused refinement map of EJC region from the human minor spliceosome branching-completed C complex
Method: EM (single particle) / Resolution: 3.59 Å

EMDB-81137: Focused refinement map of RBM48 region from the human minor spliceosome exon-ligation-ready C* complex
Method: EM (single particle) / Resolution: 3.19 Å

EMDB-81138: Focused refinement map of SYF region from the human minor spliceosome exon-ligation-ready C* complex
Method: EM (single particle) / Resolution: 3.48 Å

EMDB-81139: Human minor spliceosome exon-ligation-ready C* complex (prior to step-II) with stably-bound WDR25 region
Method: EM (single particle) / Resolution: 3.13 Å

EMDB-81140: Human minor spliceosome exon-ligation-ready C* complex (prior to step-II) with stably-bound BRR2 region
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-81143: Human minor spliceosome exon-ligation-ready C* complex (prior to step-II) with stably-bound IBC region
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-81144: Focused refinement map of PRP22 region from the human minor spliceosome exon-ligation-ready C* complex
Method: EM (single particle) / Resolution: 4.23 Å

EMDB-81145: Focused refinement map of EJC region from the human minor spliceosome exon-ligation-ready C* complex
Method: EM (single particle) / Resolution: 4.01 Å

EMDB-81204: Focused refinement map of IBC region from the human minor spliceosome branching-completed C complex
Method: EM (single particle) / Resolution: 4.41 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ZN:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsSPLICING / minor spliceosome; U12-dependent spliceosome; U12-type splicing; step-I spliceosome; SPLICING; branching; snRNP; U12; U5; U6atac; intron lariat / minor spliceosome; U12-dependent spliceosome; U12-type splicing; step-II; SPLICING; exon ligation; snRNP; U12; U5; U6atac; intron lariat; splice site; exon

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