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- EMDB-81143: Human minor spliceosome exon-ligation-ready C* complex (prior to ... -

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Basic information

Entry
Database: EMDB / ID: EMD-81143
TitleHuman minor spliceosome exon-ligation-ready C* complex (prior to step-II) with stably-bound IBC region
Map dataoverall map, not sharpened
Sample
  • Complex: Human minor spliceosome exon-ligation-ready C* complex (prior to step-II)
KeywordsRNA splicing / spliceosome / U12-type splicing / minor spliceosome / snRNP / snRNA / PRP22 / intron-binding complex / IBC / Aquarius / ATPase-helicase / C* complex / exon ligation / Step-II / spliceosome remodeling / SPLICING
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsBai R / Wan R
Funding support China, 3 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32522048 China
National Natural Science Foundation of China (NSFC) China
National Natural Science Foundation of China (NSFC)32171214 China
CitationJournal: Vita / Year: 2026
Title: Molecular mechanism of the catalysis for U12-type splicing by the human
Authors: Bai R / Guo H / Sun R / Zhu Y / Shi Y / Wan R
History
DepositionMay 29, 2026-
Header (metadata) releaseAug 12, 2026-
Map releaseAug 12, 2026-
UpdateAug 12, 2026-
Current statusAug 12, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_81143.png

Downloads & links

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Map

FileDownload / File: emd_81143.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationoverall map, not sharpened
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 640 pix.
= 689.472 Å		1.08 Å/pix.
x 640 pix.
= 689.472 Å		1.08 Å/pix.
x 640 pix.
= 689.472 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.0773 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.33
Minimum - Maximum-0.25102258 - 1.4078407
Average (Standard dev.)0.000017811693 (±0.047628284)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions640640640
Spacing640640640
CellA=B=C: 689.472 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: half map 1

Fileemd_81143_half_map_1.map
Annotationhalf map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map 2

Fileemd_81143_half_map_2.map
Annotationhalf map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Human minor spliceosome exon-ligation-ready C* complex (prior to ...

EntireName: Human minor spliceosome exon-ligation-ready C* complex (prior to step-II)
Components
  • Complex: Human minor spliceosome exon-ligation-ready C* complex (prior to step-II)

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Supramolecule #1: Human minor spliceosome exon-ligation-ready C* complex (prior to ...

SupramoleculeName: Human minor spliceosome exon-ligation-ready C* complex (prior to step-II)
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.9
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.9000000000000001 µm / Nominal defocus min: 1.3 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: EMDB MAP
EMDB ID:

6864

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 30081
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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