Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The Structure of HIV-1 Rev Filaments Suggests a Bilateral Model for Rev-RRE Assembly.
Journal, issue, pages	Structure, Vol. 24, Issue 7, Page 1068-1080, Year 2016
Publish date	Jul 6, 2016
Authors	Michael A DiMattia / Norman R Watts / Naiqian Cheng / Rick Huang / J Bernard Heymann / Jonathan M Grimes / Paul T Wingfield / David I Stuart / Alasdair C Steven /
PubMed Abstract	HIV-1 Rev protein mediates the nuclear export of viral RNA genomes. To do so, Rev oligomerizes cooperatively onto an RNA motif, the Rev response element (RRE), forming a complex that engages with the ...HIV-1 Rev protein mediates the nuclear export of viral RNA genomes. To do so, Rev oligomerizes cooperatively onto an RNA motif, the Rev response element (RRE), forming a complex that engages with the host nuclear export machinery. To better understand Rev oligomerization, we determined four crystal structures of Rev N-terminal domain dimers, which show that they can pivot about their dyad axis, giving crossing angles of 90° to 140°. In parallel, we performed cryoelectron microscopy of helical Rev filaments. Filaments vary from 11 to 15 nm in width, reflecting variations in dimer crossing angle. These structures contain additional density, indicating that C-terminal domains become partially ordered in the context of filaments. This conformational variability may be exploited in the assembly of RRE/Rev complexes. Our data also revealed a third interface between Revs, which offers an explanation for how the arrangement of Rev subunits adapts to the "A"-shaped architecture of the RRE in export-active complexes.
External links	Structure / PubMed:27265851 / PubMed Central
Methods	EM (helical sym.) / X-ray diffraction
Resolution	2.3 - 8.3 Å
Structure data	EMDB-6439: Helical cryo-EM reconstruction of HIV Rev filament Method: EM (helical sym.) / Resolution: 8.3 Å PDB-5dhv: HIV-1 Rev NTD dimers with variable crossing angles Method: X-RAY DIFFRACTION / Resolution: 2.3 Å PDB-5dhx: HIV-1 Rev NTD dimers with variable crossing angles Method: X-RAY DIFFRACTION / Resolution: 2.9 Å PDB-5dhy: HIV-1 Rev NTD dimers with variable crossing angles Method: X-RAY DIFFRACTION / Resolution: 3.1 Å PDB-5dhz: HIV-1 Rev NTD dimers with variable crossing angles Method: X-RAY DIFFRACTION / Resolution: 4.3 Å
Chemicals	ChemComp-CL: Unknown entry / Chloride ChemComp-HOH: WATER / Water
Source	human immunodeficiency virus 1 oryctolagus cuniculus (rabbit)
Keywords	IMMUNE SYSTEM / HIV / helical hairpin / RNA-binding / nuclear export