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TitleCryo-EM reveals structural diversity in prolate-headed mycobacteriophage Mycofy1.
Journal, issue, pagesJ Mol Biol, Page 169126, Year 2025
Publish dateApr 3, 2025
AuthorsXiangyun Li / Qianqian Shao / Lin Li / Linlin Xie / Zhiyang Ruan / Qianglin Fang /
PubMed AbstractMycobacteriophages show promise in treating antibiotic-resistant mycobacterial infections. Here, we isolated Mycofy1, a mycobacteriophage, using M. smegmatis as a host. Cryo-EM analysis revealed that ...Mycobacteriophages show promise in treating antibiotic-resistant mycobacterial infections. Here, we isolated Mycofy1, a mycobacteriophage, using M. smegmatis as a host. Cryo-EM analysis revealed that Mycofy1 possesses a prolate head and a long non-contractile tail. We determined structures of its head, head-to-tail interface, terminator, and tail tube to resolutions of ∼3.5 Å. Unexpectedly, we identified two distinct types of prolate head structures, exhibiting a 36-degree relative rotation in the top cap region. Additionally, the head-to-tail interface demonstrated flexibility. Our structures provide the first high-resolution cryo-EM data of a mycobacteriophage with a prolate head, as well as detailed structural information of the head-to-tail interface and head-proximal tail region in this phage group. These findings advance our understanding of assembly mechanisms in tailed bacteriophages.
External linksJ Mol Biol / PubMed:40187685
MethodsEM (single particle)
Resolution3.42 - 3.83 Å
Structure data

63432

9lw6

EMDB-63432, PDB-9lw6:
Top cap of bacteriophage Mycofy1 mature head (C5 symmetry)
Method: EM (single particle) / Resolution: 3.42 Å

63433

9lw7

EMDB-63433, PDB-9lw7:
Midsection of bacteriophage Mycofy1 mature head (C5 symmetry)
Method: EM (single particle) / Resolution: 3.52 Å

63434

9lw8

EMDB-63434, PDB-9lw8:
Bottom cap of bacteriophage Mycofy1 mature head (C5 symmetry)
Method: EM (single particle) / Resolution: 3.53 Å

63435

9lw9

EMDB-63435, PDB-9lw9:
Bacteriophage Mycofy1 proximal head-to-tail interface (C6 symmetry)
Method: EM (single particle) / Resolution: 3.46 Å

63436

9lwa

EMDB-63436, PDB-9lwa:
Bacteriophage Mycofy1 distal head-to-tail interface (C6 symmetry)
Method: EM (single particle) / Resolution: 3.83 Å

Source
  • mycolicibacterium phage mycofy1 (virus)
KeywordsVIRAL PROTEIN / Mycobacterium / bacteriophage / prolate head / major capsid protein / VIRUS / head-to-tail connector / portal protein / adaptor protein / head-to-tail interface / connector protein / terminator protein / tail tube protein

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