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TitleStructure of the pre-initiation complex explains CMGE biogenesis
Journal, issue, pagesNature, Year 2026
Publish dateJun 9, 2025 (structure data deposition date)
AuthorsPuehringer T / Canal B / Palm G / Butryn A / Couves EC / Willhoft O / Lewis JS / Diffley JFX / Costa A
External linksNature / Search PubMed
MethodsEM (single particle)
Resolution2.7 - 3.7 Å
Structure data

EMDB-53970: pre-Initiation Complex on ARS1 DNA (monomer)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-53971: Pre-Initiation Complex on ARS1 DNA (dimer)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-53972: Phospho-DH bound by Sld3-MBD on ARS1 DNA
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-53973: Phospho-MCM double hexamer bound to Sld3-Sld7-Cdc45 on ARS1 DNA
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-56898: sCMGE assembled on ARS1 DNA with Sld2 and RPA
Method: EM (single particle) / Resolution: 2.7 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)

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