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TitleCytoplasmic lattices are megadalton storage complexes in mammalian oocytes.
Journal, issue, pagesNature, Year 2026
Publish dateApr 15, 2026
AuthorsZeynep Ilgın Kılıç / Joyce van Loenhout / Marten Chaillet / Robert M van Es / Paula Sobrevals Alcaraz / Harmjan R Vos / Willem E M Noteborn / Miguel Ricardo Leung /
PubMed AbstractMammalian oocytes store proteins for embryonic development on abundant structures called cytoplasmic lattices (CPLs). However, the mechanisms by which they achieve this are unclear, largely because ...Mammalian oocytes store proteins for embryonic development on abundant structures called cytoplasmic lattices (CPLs). However, the mechanisms by which they achieve this are unclear, largely because the molecular composition of the lattices themselves is unknown. Here we use cryo-electron microscopy and artificial intelligence-based modelling to reveal the molecular architecture and protein composition of native CPLs from mouse oocytes. We find that CPLs are formed by at least 13 different proteins that assemble into a megadalton-scale complex, including multiple copies of maternal effect factors such as PADI6 and the subcortical maternal complex. We show that proteins that are essential for early embryonic development are structural components of the CPLs, including the cytoskeletal proteins α-tubulin and β-tubulin, which are incorporated into CPLs as unpolymerized dimers, and an array of ubiquitination factors such as the epigenetic regulator and E3 ligase UHRF1, ubiquitin-conjugating E2 enzymes, and ubiquitin ligase substrate adapters. This represents an elegant molecular mechanism by which oocytes stockpile vital proteins through direct incorporation into highly stable supramolecular assemblies. Our findings provide a structural framework for understanding how disrupting stored maternal factors leads to infertility and developmental defects.
External linksNature / PubMed:41986725
MethodsEM (single particle)
Resolution4.1 - 4.2 Å
Structure data

54838

9sfp

EMDB-54838, PDB-9sfp:
Native cytoplasmic lattices from mouse oocytes
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-54839: native cytoplasmic lattices from mouse oocytes, one asymmetric unit, consensus refinement
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-54840: native cytoplasmic lattices from mouse oocytes, one asymmetric unit, local refinement of PADI6 region
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-54841: native cytoplasmic lattices from mouse oocytes, one asymmetric unit, local refinement of SCMC region
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-54842: native cytoplasmic lattices from mouse oocytes, one asymmetric unit, local refinement of tubulin region
Method: EM (single particle) / Resolution: 4.1 Å

Source
  • mus musculus (house mouse)
KeywordsSTRUCTURAL PROTEIN / oocyte / embryo / cytoskeleton / tubulin / ubiquitin

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