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Open data
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Basic information
| Entry | Database: PDB / ID: 9sfp | ||||||||||||||||||
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| Title | Native cytoplasmic lattices from mouse oocytes | ||||||||||||||||||
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Keywords | STRUCTURAL PROTEIN / oocyte / embryo / cytoskeleton / tubulin / ubiquitin | ||||||||||||||||||
| Function / homology | Function and homology informationregulation of translation by machinery localization / Signaling by BMP / subcortical maternal complex / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / establishment of organelle localization / Chromatin modifying enzymes / protein storage / structural constituent of cytoplasmic lattice ...regulation of translation by machinery localization / Signaling by BMP / subcortical maternal complex / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / establishment of organelle localization / Chromatin modifying enzymes / protein storage / structural constituent of cytoplasmic lattice / cytoplasmic lattice / cortical granule exocytosis / E3 ubiquitin ligases ubiquitinate target proteins / endoplasmic reticulum localization / Carboxyterminal post-translational modifications of tubulin / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Intraflagellar transport / establishment or maintenance of apical/basal cell polarity / ooplasm / COPI-independent Golgi-to-ER retrograde traffic / PINK1-PRKN Mediated Mitophagy / Inactivation of CSF3 (G-CSF) signaling / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / Regulation of TNFR1 signaling / cytoplasm organization / histone H3 ubiquitin ligase activity / IKK complex recruitment mediated by RIP1 / COPI-mediated anterograde transport / Aggrephagy / spermatogonial cell division / Kinesins / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / cortical granule / Resolution of Sister Chromatid Cohesion / PKR-mediated signaling / The role of GTSE1 in G2/M progression after G2 checkpoint / RHO GTPases activate IQGAPs / Recycling pathway of L1 / COPI-dependent Golgi-to-ER retrograde traffic / RHO GTPases Activate Formins / apical cortex / axonemal microtubule / positive regulation of meiotic nuclear division / Separation of Sister Chromatids / regulation of RNA stability / positive regulation of embryonic development / organelle transport along microtubule / Hedgehog 'off' state / Peroxisomal protein import / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / hemi-methylated DNA-binding / Anchoring of the basal body to the plasma membrane / Recruitment of NuMA to mitotic centrosomes / regulation of establishment of protein localization / forebrain morphogenesis / AURKA Activation by TPX2 / Regulation of PLK1 Activity at G2/M Transition / cerebellar cortex morphogenesis / Neddylation / glial cell differentiation / dentate gyrus development / neuron projection arborization / embryonic pattern specification / (E3-independent) E2 ubiquitin-conjugating enzyme / MHC class II antigen presentation / embryonic cleavage / protein K6-linked ubiquitination / mitochondrion localization / flagellated sperm motility / establishment of spindle localization / regulation of epithelial cell proliferation / intermediate filament cytoskeleton / Antigen processing: Ubiquitination & Proteasome degradation / : / methyl-CpG binding / protein K11-linked ubiquitination / fertilization / histone H3K9me2/3 reader activity / pyramidal neuron differentiation / response to L-glutamate / epigenetic programming in the zygotic pronuclei / positive regulation of dendrite development / centrosome cycle / positive regulation of neurogenesis / E2 ubiquitin-conjugating enzyme / tubulin complex / smoothened signaling pathway / mitotic spindle assembly checkpoint signaling / positive regulation of double-strand break repair / negative regulation of gene expression via chromosomal CpG island methylation / regulation of synapse organization / startle response / exocytosis / motor behavior / microtubule polymerization / positive regulation of protein metabolic process Similarity search - Function | ||||||||||||||||||
| Biological species | ![]() | ||||||||||||||||||
| Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.2 Å | ||||||||||||||||||
Authors | Kilic, Z.I. / van Loenhout, J. / Chaillet, M. / Noteborn, W.E.M. / Leung, M.R. | ||||||||||||||||||
| Funding support | Netherlands, 1items
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Citation | Journal: To Be PublishedTitle: Maternal factors assemble into megadalton storage complexes in mammalian oocytes Authors: Kilic, Z.I. / van Loenhout, J. / Chaillet, M. / Noteborn, W.E.M. / Leung, M.R. | ||||||||||||||||||
| History |
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Structure visualization
| Structure viewer | Molecule: Molmil Jmol/JSmol |
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Downloads & links
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Download
| PDBx/mmCIF format | 9sfp.cif.gz | 2.5 MB | Display | PDBx/mmCIF format |
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| PDB format | pdb9sfp.ent.gz | Display | PDB format | |
| PDBx/mmJSON format | 9sfp.json.gz | Tree view | PDBx/mmJSON format | |
| Others | Other downloads |
-Validation report
| Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sf/9sfp ftp://data.pdbj.org/pub/pdb/validation_reports/sf/9sfp | HTTPS FTP |
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-Related structure data
| Related structure data | ![]() 54838MC M: map data used to model this data C: citing same article ( |
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| Similar structure data | Similarity search - Function & homology F&H Search |
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Links
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Assembly
| Deposited unit | ![]()
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| 1 | ![]()
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Components
-Protein , 11 types, 24 molecules ABCDFKLMNOPQRSTUVWYabcde
| #1: Protein | Mass: 54036.707 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Protein | | Mass: 48055.301 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Protein | Mass: 108001.281 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #6: Protein | Mass: 18481.295 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #7: Protein | Mass: 76854.109 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Source: (natural) ![]() #8: Protein | Mass: 65187.332 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #9: Protein | | Mass: 50188.441 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P68369, Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement #10: Protein | | Mass: 49877.824 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #11: Protein | | Mass: 16706.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P61079, E2 ubiquitin-conjugating enzyme, (E3-independent) E2 ubiquitin-conjugating enzyme #12: Protein | | Mass: 88436.805 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: Q8VDF2, RING-type E3 ubiquitin transferase #13: Protein | | Mass: 25643.373 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-NACHT, LRR and PYD domains-containing protein ... , 2 types, 3 molecules HIJ
| #4: Protein | Mass: 131468.547 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #5: Protein | | Mass: 113527.188 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Details
| Has protein modification | N |
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-Experimental details
-Experiment
| Experiment | Method: ELECTRON MICROSCOPY |
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| EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
| Component | Name: native cytoplasmic lattices from mouse oocytes / Type: COMPLEX / Entity ID: all / Source: NATURAL |
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| Molecular weight | Experimental value: NO |
| Source (natural) | Organism: ![]() |
| Buffer solution | pH: 7.2 |
| Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
| Specimen support | Grid material: COPPER / Grid type: Quantifoil R2/1 |
| Vitrification | Cryogen name: ETHANE-PROPANE |
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Electron microscopy imaging
| Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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| Microscopy | Model: TFS KRIOS |
| Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
| Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 81000 X / Nominal defocus max: 2500 nm / Nominal defocus min: 500 nm / Cs: 2.7 mm / Alignment procedure: COMA FREE |
| Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
| Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
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| CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||
| Symmetry | Point symmetry: C2 (2 fold cyclic) | ||||||||||||||||||||||||||||||||||||
| 3D reconstruction | Resolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 424595 / Symmetry type: POINT | ||||||||||||||||||||||||||||||||||||
| Atomic model building | Protocol: FLEXIBLE FIT |
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About Yorodumi






Netherlands, 1items
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FIELD EMISSION GUN