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TitleMolecular basis for multidrug efflux by an anaerobic RND transporter.
Journal, issue, pagesbioRxiv, Year 2025
Publish dateApr 5, 2025
AuthorsRyan Lawrence / Mohd Athar / Muhammad R Uddin / Christopher Adams / Joana S Sousa / Oliver Durrant / Sophie Lellman / Lucy Sutton / C William Keevil / Nisha Patel / Christine Prosser / David McMillan / Helen I Zgurskaya / Attilio V Vargiu / Zainab Ahdash / Eamonn Reading
PubMed AbstractBacteria can resist antibiotics and toxic substances within demanding ecological settings, such as low oxygen, extreme acid, and during nutrient starvation. MdtEF, a proton motive force-driven efflux ...Bacteria can resist antibiotics and toxic substances within demanding ecological settings, such as low oxygen, extreme acid, and during nutrient starvation. MdtEF, a proton motive force-driven efflux pump from the resistance-nodulation-cell division (RND) superfamily, is upregulated in these conditions but its molecular mechanism is unknown. Here, we report cryo-electron microscopy structures of Escherichia coli multidrug transporter MdtF within native-lipid nanodiscs, including a single-point mutant with an altered multidrug phenotype and associated substrate-bound form. We reveal that drug binding domain and channel conformational plasticity likely governs promiscuous substrate specificity, analogous to its closely related, constitutively expressed counterpart, AcrB. Whereas we discover distinct transmembrane state transitions within MdtF, which create a more engaged proton relay network, altered drug transport allostery and an acid-responsive increase in efflux efficiency. Physiologically, this provides means of xenobiotic and metabolite disposal within remodelled cell membranes that presage encounters with acid stresses, as endured in the gastrointestinal tract.
External linksbioRxiv / PubMed:40236129 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.56 Å
Structure data

53281

9qpr

EMDB-53281, PDB-9qpr:
Single particle cryo-EM structure of the multidrug efflux pump MdtF from Escherichia coli
Method: EM (single particle) / Resolution: 3.56 Å

53282

9qps

EMDB-53282, PDB-9qps:
Single particle cryo-EM structure of MdtF V610F
Method: EM (single particle) / Resolution: 3.28 Å

53283

9qpt

EMDB-53283, PDB-9qpt:
Single particle cryo-EM structure of MdtF V610F with bound Rhodamine 6G
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

ChemComp-D12:
DODECANE

ChemComp-RHQ:
RHODAMINE 6G

Source
  • escherichia coli k-12 (bacteria)
KeywordsMEMBRANE PROTEIN / multidrug / pump / anaerobic / lipid

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