EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV.
ジャーナル・号・ページ	Structure, Vol. 33, Issue 10, Page 1717-11727.e4, Year 2025
掲載日	2025年10月2日
著者	Dimple Karia / Adrian F Koh / Wen Yang / Victoria I Cushing / Benjamin Basanta / Daniel B Mihaylov / Sagar Khavnekar / Ondřej Vyroubal / Miloš Malínský / Ondřej Sháněl / Vojtěch Doležal / Jürgen Plitzko / Lingbo Yu / Gabriel C Lander / A Radu Aricescu / Basil J Greber / Abhay Kotecha /
PubMed 要旨	Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report sub-3 Å resolution structures using the 100 keV ...Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report sub-3 Å resolution structures using the 100 keV Tundra cryo-TEM, equipped with the Falcon C direct electron detector (DED). This system combines advanced optics, extreme-brightness field emission gun (XFEG), and SP-TWIN lens to enhance coherence and resolution. The semi-automated loader reduced contamination and drift, enabling extended data collection, while the high detective quantum efficiency (DQE) of Falcon C improved signal-to-noise ratio. We validated performance by determining structures of biological samples, including apoferritin (2.1 Å), T20S proteasome (2.7 Å), GABA receptor (2.8 Å), hemoglobin (5.0 Å), transthyretin (3.5 Å), and AAV9 capsid (2.8 Å), spanning 50 kDa-3.9 MDa. This work highlights the potential of 100 keV transmission electron microscopes (TEMs) to make cryo-EM more accessible. It sets a precedent for using lower voltage TEMs not only for screening, but also for high-resolution protein structure determination.
リンク	Structure / PubMed:40744008 / PubMed Central
手法	EM (単粒子)
解像度	2.1 - 5.0 Å
構造データ	EMDB-13769: CryoEM structure of Apoferritin at 2.6A from Tundra, 100kV microscope 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-13816: CryoEM structure of GABA(A)R-beta3 homopentamer at 3.4A from Tundra, 100kV microscope 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-14249: CryoEM structure of T20S proteasome at 3A from Tundra, 100kV microscope 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-51463: ApoF at 2.2A resolution imaged at 0.55A/pixel on Falcon C 手法: EM (単粒子) / 解像度: 2.2 Å EMDB-51481: Apoferritin at 2.1A from Falcon C imaged at 0.7Apix 手法: EM (単粒子) / 解像度: 2.1 Å EMDB-51483: Apoferritin at 2.2A from Falcon C imaged at 0.9Apix 手法: EM (単粒子) / 解像度: 2.2 Å EMDB-51487: T20S Proteosome at 2.7A from Falcon C imaged at 0.7Apix 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-51503: T20S Proteosome at 2.9A from Falcon C imaged at 0.9Apix 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-51506: GABAA receptor at 2.8A from Falcon C imaged at 0.7Apix 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-51508: GABAA receptor at 2.8A from Falcon C imaged at 0.9Apix 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-51511: Rabbit muscle aldolase at 3A resolution imaged at 0.55A/pix 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-51512: Rabbit muscle aldolase at 2.8A imaged at 0.7Apix 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-51519: CAK complex at 4A imaged on Falcon C 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-51525: Human Haemoglobin at 5A imaged on Falcon C 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-51526: Human Transthyretin at 3.5A imaged on Falcon C 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-51540: Adeno-associated Virus 9 at 2.8A imaged on Falcon C 手法: EM (単粒子) / 解像度: 2.8 Å
由来	Escherichia coli (大腸菌) HEK 293S cells (大腸菌) Thermoplasma acidophilum (好酸性) Mus musculus (ハツカネズミ) Homo sapiens (ヒト) Oryctolagus cuniculus (ウサギ)