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Structure paper

TitleThe random-model method enables ab initio 3D reconstruction of asymmetric particles and determination of particle symmetry.
Journal, issue, pagesJ Struct Biol, Vol. 171, Issue 2, Page 216-222, Year 2010
Publish dateMar 28, 2010
AuthorsEduardo Sanz-García / Aaron B Stewart / David M Belnap /
PubMed AbstractModel-based, 3D reconstruction techniques depend on reliable starting models. We present an extension of the random-model method (RMM) that allows the ab initio generation of suitable starting models ...Model-based, 3D reconstruction techniques depend on reliable starting models. We present an extension of the random-model method (RMM) that allows the ab initio generation of suitable starting models directly from un-averaged, experimental images of asymmetric or symmetric particles. Therefore, the asymmetric RMM can also be used to determine point-group symmetry. The procedure is facilitated by the use of (a) variable angular step-sizes during iterative origin and orientation searches, (b) high numbers of particle images, and (c) highly defocused images. The method is inhibited by mixed-handedness orientation assignments and by particles with inconspicuous features. For symmetric particles, symmetric RMMs can overcome these deficiencies.
External linksJ Struct Biol / PubMed:20353825 / PubMed Central
MethodsEM (single particle)
Resolution18.0 - 35.0 Å
Structure data

EMDB-5141:
Ab initio reconstruction of the E. coli 70S ribosome complex (70S-fMet-tRNAfMet-Phe-tRNAPhe-EF-Tu-GDP-kirromycin) via the asymmetric random-model method.
Method: EM (single particle) / Resolution: 23.0 Å

EMDB-5142:
Ab initio reconstruction of the avian polyomavirus via the asymmetric random-model method
Method: EM (single particle) / Resolution: 35.0 Å

EMDB-5143:
Ab initio reconstruction of GroEL via the asymmetric random-model method
Method: EM (single particle) / Resolution: 18.0 Å

EMDB-5144:
Ab initio reconstruction of a poliovirus-receptor complex via the asymmetric random-model method
Method: EM (single particle) / Resolution: 30.0 Å

Source
  • Escherichia coli (E. coli)

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