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TitleHelical superstructures between amyloid and collagen in cardiac fibrils from a patient with AL amyloidosis.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 6359, Year 2024
Publish dateJul 28, 2024
AuthorsTim Schulte / Antonio Chaves-Sanjuan / Valentina Speranzini / Kevin Sicking / Melissa Milazzo / Giulia Mazzini / Paola Rognoni / Serena Caminito / Paolo Milani / Chiara Marabelli / Alessandro Corbelli / Luisa Diomede / Fabio Fiordaliso / Luigi Anastasia / Carlo Pappone / Giampaolo Merlini / Martino Bolognesi / Mario Nuvolone / Rubén Fernández-Busnadiego / Giovanni Palladini / Stefano Ricagno /
PubMed AbstractSystemic light chain (LC) amyloidosis (AL) is a disease where organs are damaged by an overload of a misfolded patient-specific antibody-derived LC, secreted by an abnormal B cell clone. The high LC ...Systemic light chain (LC) amyloidosis (AL) is a disease where organs are damaged by an overload of a misfolded patient-specific antibody-derived LC, secreted by an abnormal B cell clone. The high LC concentration in the blood leads to amyloid deposition at organ sites. Indeed, cryogenic electron microscopy (cryo-EM) has revealed unique amyloid folds for heart-derived fibrils taken from different patients. Here, we present the cryo-EM structure of heart-derived AL amyloid (AL59) from another patient with severe cardiac involvement. The double-layered structure displays a u-shaped core that is closed by a β-arc lid and extended by a straight tail. Noteworthy, the fibril harbours an extended constant domain fragment, thus ruling out the variable domain as sole amyloid building block. Surprisingly, the fibrils were abundantly concatenated with a proteinaceous polymer, here identified as collagen VI (COLVI) by immuno-electron microscopy (IEM) and mass-spectrometry. Cryogenic electron tomography (cryo-ET) showed how COLVI wraps around the amyloid forming a helical superstructure, likely stabilizing and protecting the fibrils from clearance. Thus, here we report structural evidence of interactions between amyloid and collagen, potentially signifying a distinct pathophysiological mechanism of amyloid deposits.
External linksNat Commun / PubMed:39069558 / PubMed Central
MethodsEM (single particle) / EM (helical sym.) / EM (tomography)
Resolution3.6 - 15.0 Å
Structure data

EMDB-18689: Maps of Collagen VI half- and full-beads
Method: EM (single particle) / Resolution: 15.0 Å

50270

9faa

EMDB-50270, PDB-9faa:
Cryo-EM structure of cardiac collagen-associated amyloid AL59
Method: EM (helical sym.) / Resolution: 3.6 Å

50272

9fac

EMDB-50272, PDB-9fac:
Additional cryo-EM structure of cardiac amyloid AL59 - mixed polymorph
Method: EM (helical sym.) / Resolution: 3.9 Å

EMDB-51031: Cryo-electron tomogram of AL59 amyloids interacting with collagen VI
Method: EM (tomography)

EMDB-51032: Cryo-electron tomogram of AL59 amyloids interacting with collagen VI
Method: EM (tomography)

EMDB-51033: Cryo-electron tomogram of AL59 amyloids interacting with collagen VI
Method: EM (tomography)

EMDB-51038: Cryo-electron tomogram of AL59 amyloids interacting with collagen VI
Method: EM (tomography)

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
KeywordsPROTEIN FIBRIL / systemic AL amyloid fibril

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