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TitleStructures of the Escherichia coli type 1 pilus during pilus rod assembly and after assembly termination.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 4988, Year 2025
Publish dateMay 29, 2025
AuthorsPaul Bachmann / Pavel Afanasyev / Daniel Boehringer / Rudi Glockshuber /
PubMed AbstractUropathogenic Escherichia coli strains use filamentous type 1 pili to adhere to and invade uroepithelial cells. The pilus consists of a flexible tip fibrillum, formed by the adhesin FimH and the ...Uropathogenic Escherichia coli strains use filamentous type 1 pili to adhere to and invade uroepithelial cells. The pilus consists of a flexible tip fibrillum, formed by the adhesin FimH and the subunits FimG and FimF. The pilus rod is a helical assembly of up to 3000 copies of the main subunit FimA, terminated by a single copy of the subunit FimI that anchors the rod to the assembly platform FimD in the outer membrane. Although type 1 pilus assembly can be completely reconstituted in vitro, the precise mechanism of assembly termination on FimD is still unknown. Here, we present cryo-electron microscopy structures of the fully assembled pilus with all its components prior to and after incorporation of FimI, capped with the assembly chaperone FimC. The structures reveal that FimD positions the proximal end of the pilus rod at an angle of ca. 50 degrees relative to the plane of the outer membrane. Specific interactions between FimI and FimC, absent in the equivalent FimA-FimC interface of the non-terminated pilus, stabilize the assembly-terminated state. In addition, we present structures of the transition region between the tip fibrillum and the helical rod, showing how FimF aligns the tip fibrillum along the rod axis.
External linksNat Commun / PubMed:40442073 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 6.4 Å
Structure data

50751

9ftt

EMDB-50751, PDB-9ftt:
Cryo-EM structure of the type 1 pilus rod as part of the FimA-bound usher complex (FimDHGFAnC)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-50755: Cryo-EM map of the type 1 pilus complex including pilus rod and FimA-bound assembly platform
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-50773: Cryo-EM map of the type 1 chaperone-usher pilus tip and rod - Conformer 1
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-50796: Cryo-EM map of the type 1 chaperone-usher pilus tip and rod - Conformer 2
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-50806: Cryo-EM map of the type 1 chaperone-usher pilus tip and rod - Conformer 3
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-50809: Cryo-EM map of the type 1 chaperone-usher pilus tip and rod - Conformer 4
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-50810: Cryo-EM map of the type 1 pilus complex including pilus rod and FimI-bound assembly platform
Method: EM (single particle) / Resolution: 6.4 Å

EMDB-50812: Cryo-EM map of the type 1 pilus complex including pilus rod and FimI-bound assembly platform after incorporation of two FimI subunits
Method: EM (single particle) / Resolution: 4.3 Å

50828

9fw9

EMDB-50828, PDB-9fw9:
Cryo-EM structure of the type 1 pilus assembly platform as part of the FimA-bound chaperone-usher pilus complex (FimDHGFAnC - body 2)
Method: EM (single particle) / Resolution: 3.9 Å

50829

9fwb

EMDB-50829, PDB-9fwb:
Cryo-EM structure of the type 1 pilus assembly platform as part of the FimA-bound chaperone-usher pilus complex (Local refinement including FimD, FimC, FimAn and FimAn-1)
Method: EM (single particle) / Resolution: 3.5 Å

50839

9fx0

EMDB-50839, PDB-9fx0:
Cryo-EM structure of the type 1 pilus tip-to-rod transition
Method: EM (single particle) / Resolution: 3.1 Å

50843

9fx8

EMDB-50843, PDB-9fx8:
Cryo-EM structure of the FimI-bound type 1 pilus assembly platform complex - Local refinement
Method: EM (single particle) / Resolution: 3.6 Å

50846

9fxa

EMDB-50846, PDB-9fxa:
Cryo-EM structure of the type 1 pilus rod as part of the FimI-bound usher complex (FimDHGFAnIC - body 1)
Method: EM (single particle) / Resolution: 4.0 Å

50847

9fxb

EMDB-50847, PDB-9fxb:
Cryo-EM structure of the type 1 pilus assembly platform as part of the FimI-bound chaperone-usher pilus complex (FimDHGFAnIC - body 2)
Method: EM (single particle) / Resolution: 4.3 Å

50853

9fxs

EMDB-50853, PDB-9fxs:
Cryo-EM structure of the type 1 pilus complex including pilus rod and FimI-bound assembly platform after incorporation of two FimI subunits - Local refinement
Method: EM (single particle) / Resolution: 4.2 Å

50861

9fy9

EMDB-50861, PDB-9fy9:
Cryo-EM structure of the type 1 chaperone-usher pilus FimD-tip complex (FimDHGFC) - Conformer 1
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-50954: Cryo-EM map of the type 1 chaperone-usher pilus FimD-tip complex - Conformer 2
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • escherichia coli (E. coli)
KeywordsCELL ADHESION / chaperone / usher / pilus / rod / MEMBRANE PROTEIN / tip / termination

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