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-Structure paper
| タイトル | Structural insights into cauliflower mitoribosome in translation state and in association with a late assembly factor. |
|---|---|
| ジャーナル・号・ページ | Nat Commun, Vol. 16, Issue 1, Page 10839, Year 2025 |
| 掲載日 | 2025年12月2日 |
 著者 | Vasileios Skaltsogiannis / Philippe Wolff / Tan-Trung Nguyen / Nicolas Corre / David Pflieger / Todd Blevins / Yaser Hashem / Philippe Giegé / Florent Waltz /   |
| PubMed 要旨 | Ribosomes are key molecular machines that translate mRNA into proteins. Mitoribosomes are specific ribosomes found in mitochondria, which have been shown to be remarkably diverse across eukaryotic ...Ribosomes are key molecular machines that translate mRNA into proteins. Mitoribosomes are specific ribosomes found in mitochondria, which have been shown to be remarkably diverse across eukaryotic lineages. In plants, they possess unique features, including additional rRNA domains stabilized by plant-specific proteins. However, the structural specificities of plant mitoribosomes in translation state remained unknown. We used cryo-electron microscopy to provide a high-resolution structural characterization of the cauliflower mitoribosome, in translating and maturation states. The structure reveals the mitoribosome bound with a tRNA in the peptidyl site, along with a segment of mRNA and a nascent polypeptide. Moreover, using structural data, nanopore sequencing and mass spectrometry, we identify a set of 19 ribosomal RNA modifications. Additionally, we observe a late assembly intermediate of the small ribosomal subunit, in complex with the RsgA assembly factor. This reveals how a plant-specific extension of RsgA blocks the mRNA channel to prevent premature mRNA association before complete small subunit maturation. Our findings elucidate key aspects of translation in angiosperm plant mitochondria, revealing its distinct features compared to other eukaryotic lineages. |
 リンク | Nat Commun / PubMed:41330901 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 1.99 - 4.32 Å |
| 構造データ | EMDB-50011, PDB-9evs:  EMDB-50012: Structure of the flowering plant mitoribosome with P-site tRNA, large subunit focused  EMDB-50013: Structure of the flowering plant mitoribosome with P-site tRNA, small subunit focused  EMDB-50015: Structure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA, head focused  EMDB-50016: Structure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA, RsgA focused  EMDB-50017: Structure of the small subunit of the flowering plant mitoribosome with the maturation factor RsgA, body focused  EMDB-51703: High resolution structure of B. oleracea mitoribosome - unfocused  EMDB-51704: High resolution structure of B. oleracea mitoribosome - Small subunit body focused  EMDB-51705: High resolution structure of B. oleracea mitoribosome - Small subunit head focus  EMDB-51706: High resolution structure of B. oleracea mitoribosome - Small subunit head S3 area focus  EMDB-51707: High resolution structure of B. oleracea mitoribosome - Small subunit foot rPPR10 focus  EMDB-51708: High resolution structure of B. oleracea mitoribosome - Small subunit foot rPPR1 focus  EMDB-51709: High resolution structure of B. oleracea mitoribosome - Small subunit body protuberance focus  EMDB-51710: High resolution structure of B. oleracea mitoribosome - Large subunit focus  EMDB-51711: High resolution structure of B. oleracea mitoribosome - Large subunit Central Protuberance focus  EMDB-51712: High resolution structure of B. oleracea mitoribosome - Large subunit L7/12 stalk focus  EMDB-51713: High resolution structure of B. oleracea mitoribosome - Large subunit back protuberance focus  EMDB-51714: High resolution structure of B. oleracea mitoribosome - Large subunit rPPR5 focus  EMDB-51715: High resolution structure of B. oleracea mitoribosome - Small subunit head extension base focus  EMDB-51716: High resolution structure of B. oleracea mitoribosome - Small subunit head extension core focus  EMDB-51717: High resolution structure of B. oleracea mitoribosome - Small subunit head extension tip focus EMDB-51718, PDB-9gyt: |
| 化合物 |  ChemComp-MG:  ChemComp-ATP:  ChemComp-CLM:  ChemComp-K:  ChemComp-ZN:  ChemComp-HOH: |
| 由来 |
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 キーワード | RIBOSOME / Mitochondria / tRNA / mitoribosome / plant / assembly factor |
brassica oleracea var. botrytis (カリフラワー)
arabidopsis thaliana (シロイヌナズナ)