EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Visualizing insecticide control of insect TRP channel function and assembly.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Page 595, Year 2025
掲載日	2025年12月14日
著者	Justin G Fedor / Ramani Kandasamy / Cheon-Gyu Park / Yang Suo / Martin Weisel / Nancy B Rankl / Alexandre Nesterov / Seok-Yong Lee /
PubMed 要旨	Insecticides are vital to combating world food shortages and transmission of vector-borne human diseases. Increasing insecticide resistance necessitates discovery of novel compounds against ...Insecticides are vital to combating world food shortages and transmission of vector-borne human diseases. Increasing insecticide resistance necessitates discovery of novel compounds against underutilized targets. Nanchung (Nan) and Inactive (Iav), the transient receptor potential vanilloid-type (TRPV) channels in insects, likely form a heteromeric channel (Nan-Iav) and are localized in mechanosensory chordotonal organs which confer gravitaxis, hearing and proprioception. Several insecticides, such as afidopyropen (AP), target Nan-Iav through unknown mechanisms. Effective against piercing-sucking (hemipteran) insects, AP disrupts chordotonal functions preventing feeding. AP can bind to Nan alone, but only Nan-Iav exhibits channel activity with agonists including endogenous nicotinamide (NAM). Despite its importance as an insecticide target, much is unknown about Nan-Iav, such as channel assembly, modulator binding sites, and Ca-dependent regulation, hampering further insecticide development. Here we present the cryo-electron microscopy structures of hemipteran Nan-Iav with calmodulin bound in the apo state and with AP and NAM bound to cytosolic ankyrin repeat domain (ARD) interfaces. Unexpectedly, we found that Nan alone can form a pentamer, stabilized through AP-mediated ARD interactions. Our study provides molecular insights into insecticide and agonist interactions with Nan-Iav, highlighting the importance of the ARD on channel function and assembly, while also probing regulation by Ca.
リンク	Nat Commun / PubMed:41392056 / PubMed Central
手法	EM (単粒子)
解像度	2.49 - 3.28 Å
構造データ	49844 9nvn EMDB-49844, PDB-9nvn: Structure of Nanchung-Inactive-Calmodulin in apo state 手法: EM (単粒子) / 解像度: 2.89 Å 49845 9nvo EMDB-49845, PDB-9nvo: Structure of Nanchung-Inactive-Calmodulin in complex with Nicotinamide 手法: EM (単粒子) / 解像度: 2.49 Å 49846 9nvp EMDB-49846, PDB-9nvp: Structure of Nanchung-Inactive-Calmodulin in complex with Nicotinamide, EDTA 手法: EM (単粒子) / 解像度: 2.85 Å 49847 9nvq EMDB-49847, PDB-9nvq: Structure of Nanchung-Inactive-Calmodulin in complex with Afidopyropen and calcium 手法: EM (単粒子) / 解像度: 3.01 Å 49848 9nvr EMDB-49848, PDB-9nvr: Structure of Nanchung-Inactive-Calmodulin in complex with Afidopyropen, EDTA 手法: EM (単粒子) / 解像度: 3.13 Å 49849 9nvs EMDB-49849, PDB-9nvs: Structure of a pentameric Nanchung in complex with Afidopyropen 手法: EM (単粒子) / 解像度: 3.28 Å
化合物	ChemComp-6OU: [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate / POPE / リン脂質YM ChemComp-LBN: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / リン脂質YM ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-D39: (2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid / 1-パルミトイル-2-オレオイルホスファチジルセリン ChemComp-NCA: NICOTINAMIDE / ニコチンアミド / 薬剤YM PDB-1b32*: OLIGO-PEPTIDE BINDING PROTEIN (OPPA) COMPLEXED WITH KMK
由来	homo sapiens (ヒト) halyomorpha halys (クサギカメムシ)
キーワード	TRANSPORT PROTEIN / Membrane protein / membrane channel