EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Human monoclonal antibodies that target clade 2.3.4.4b H5N1 hemagglutinin.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Page 135, Year 2025
掲載日	2025年12月13日
著者	Garazi Peña Alzua / André Nicolás León / Temima Yellin / Disha Bhavsar / Madhumathi Loganathan / Kaitlyn Bushfield / Philip J M Brouwer / Alesandra J Rodriguez / Trushar Jeevan / Richard Webby / Christine Marizzi / Julianna Han / Andrew B Ward / J Andrew Duty / Florian Krammer /
PubMed 要旨	The highly pathogenic avian influenza H5N1 virus clade 2.3.4.4b has been spreading globally since 2022, causing mortality and morbidity in domestic and wild birds, as well as in mammals, which ...The highly pathogenic avian influenza H5N1 virus clade 2.3.4.4b has been spreading globally since 2022, causing mortality and morbidity in domestic and wild birds, as well as in mammals, which underscores its potential to cause a pandemic. Here, we generate a panel of anti-hemagglutinin (HA) human monoclonal antibodies (mAbs) against the H5 protein of clade 2.3.4.4b. To develop human chimeric antibodies, H2L2 Harbor Mice®, which express human immunoglobulin germline genes, were immunized with H5 and N1 recombinant proteins from A/mallard/New York/22-008760-007- original/2022 H5N1 virus. Through hybridoma technology, sixteen fully human mAbs are generated, most of which show cross-reactivity against H5 proteins from different clade 2.3.4.4 virus variants. Fourteen out of the sixteen mAbs neutralize the virus in vitro. The mAbs with the strongest hemagglutination inhibition activity also demonstrate greater neutralizing capacity and show increased protective effects in vivo when administered prophylactically or therapeutically in a murine H5N1 challenge model. Using cryo-electron microscopy, we identify a cross-clonotype conserved motif that bound a hydrophobic groove on the head domain of H5 HA. Akin to mAbs against severe acute respiratory syndrome coronavirus 2 during the coronavirus 2019 pandemic, these mAbs could serve as treatments in case of a widespread H5N1 epidemic or pandemic.
リンク	Nat Commun / PubMed:41390501 / PubMed Central
手法	EM (単粒子)
解像度	3.06 - 25.0 Å
構造データ	48510 9mq1 EMDB-48510: Cryo-EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 20D10 and CR9114 PDB-9mq1: Cryo-EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 20D10 手法: EM (単粒子) / 解像度: 3.37 Å 48511 9mq2 EMDB-48511: Cryo-EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 12G1 and CR9114 PDB-9mq2: Cryo-EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 12G1 手法: EM (単粒子) / 解像度: 3.06 Å 48512 9mq3 EMDB-48512: Cryo-EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 6G1, 1A1 and CR9114 PDB-9mq3: Cryo-EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 6G1 手法: EM (単粒子) / 解像度: 3.22 Å EMDB-48679: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 1A1 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48680: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 1H2 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48681: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 6G1 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48682: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 7G4 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48683: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 7G11 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48684: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 12C11 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48685: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 12G1 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48686: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 12G8 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48687: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 12G9 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48688: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 13E8 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48689: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 17C12 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48690: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 17E3 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48692: Negative stain EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fab 20D10 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-48693: A/Red-tailed hawk/New York/NYCVH 22-8477/2022 H5 in complex with monoclonal fab 1A1 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-48694: Negative stain map of A/Red-tailed hawk/New York/NYCVH 22-8477/2022 H5 in complex with monoclonal Fab 20D10 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-48695: Negative stain map of A/Red-tailed hawk/New York/NYCVH 22-8477/2022 H5 in complex with monoclonal Fab 6G1 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-48696: Negative stain map of A/Red-tailed hawk/New York/NYCVH 22-8477/2022 H5 in complex with monoclonal Fab 12G1 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-48822: Cryo-EM map of H5 HA (A/Jiangsu/NJ210/2023) in complex with monoclonal fabs 1A1, 6G1, and CR9114. 手法: EM (単粒子) / 解像度: 3.47 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	homo sapiens (ヒト) influenza a virus (A型インフルエンザウイルス) Mus musculus (ハツカネズミ)
キーワード	VIRAL PROTEIN/IMMUNE SYSTEM / influenza / hemaglutinin / antibody-antigen complex / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex / hemagglutinin / H5 / monoclonal / complex / fab complex / viral fusion protein