Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Evolutionary adaptations of doublet microtubules in trypanosomatid parasites.
Journal, issue, pages	Science, Vol. 387, Issue 6739, Page eadr5507, Year 2025
Publish date	Mar 14, 2025
Authors	Matthew H Doran / Qingwei Niu / Jianwei Zeng / Tom Beneke / James Smith / Peter Ren / Sophia Fochler / Adrian Coscia / Johanna L Höög / Shimi Meleppattu / Polina V Lishko / Richard J Wheeler / Eva Gluenz / Rui Zhang / Alan Brown /
PubMed Abstract	The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum that contains an axoneme of dynein-bound doublet ...The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum that contains an axoneme of dynein-bound doublet microtubules (DMTs). In this work, we present cryo-electron microscopy structures of DMTs from two trypanosomatid species, and , at resolutions up to 2.7 angstrom. The structures revealed 27 trypanosomatid-specific microtubule inner proteins, a specialized dynein-docking complex, and the presence of paralogous proteins that enable higher-order periodicities or proximal-distal patterning. Leveraging the genetic tractability of trypanosomatid species, we quantified the location and contribution of each structure-identified protein to swimming behavior. Our study shows that proper B-tubule closure is critical for flagellar motility, exemplifying how integrating structural identification with systematic gene deletion can dissect individual protein contributions to flagellar motility.
External links	Science / PubMed:40080577
Methods	EM (single particle)
Resolution	2.8 - 3.6 Å
Structure data	EMDB-47607: Leishmania tarentolae 48 nm doublet microtubule consensus map Method: EM (single particle) / Resolution: 3.3 Å EMDB-47608: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.1 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47609: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.2 focused refinement Method: EM (single particle) / Resolution: 3.0 Å EMDB-47610: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.2 focused refinement Method: EM (single particle) / Resolution: 3.0 Å EMDB-47612: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.1 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47613: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.2 focused refinement Method: EM (single particle) / Resolution: 3.1 Å EMDB-47614: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.3 focused refinement Method: EM (single particle) / Resolution: 3.1 Å EMDB-47615: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.1 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47616: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.2 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47618: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.3 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47619: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.1 focused refinement Method: EM (single particle) / Resolution: 3.6 Å EMDB-47620: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.2 focused refinement Method: EM (single particle) / Resolution: 3.3 Å EMDB-47622: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.3 focused refinement Method: EM (single particle) / Resolution: 3.3 Å EMDB-47624: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.1 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47627: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.2 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47630: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.3 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47633: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.1 focused refinement Method: EM (single particle) / Resolution: 2.9 Å EMDB-47635: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.2 focused refinement Method: EM (single particle) / Resolution: 2.8 Å EMDB-47636: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.3 focused refinement Method: EM (single particle) / Resolution: 2.9 Å EMDB-47638: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.1 focused refinement Method: EM (single particle) / Resolution: 3.4 Å EMDB-47639: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.2 focused refinement Method: EM (single particle) / Resolution: 3.3 Å EMDB-47640: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.1 focused refinement Method: EM (single particle) / Resolution: 3.3 Å EMDB-47641: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.2 focused refinement Method: EM (single particle) / Resolution: 3.3 Å EMDB-47642: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.3 focused refinement Method: EM (single particle) / Resolution: 3.4 Å EMDB-47643: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.1 focused refinement Method: EM (single particle) / Resolution: 3.3 Å EMDB-47644: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.2 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47645: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.3 focused refinement Method: EM (single particle) / Resolution: 3.3 Å EMDB-47646: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.1 focused refinement Method: EM (single particle) / Resolution: 3.2 Å EMDB-47647: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.2 focused refinement Method: EM (single particle) / Resolution: 3.1 Å EMDB-47648: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.3 focused refinement Method: EM (single particle) / Resolution: 3.1 Å EMDB-47649: Leishmania tarentolae 48 nm doublet microtubule protofilament ODADC1 focused refinement Method: EM (single particle) / Resolution: 3.4 Å EMDB-47650: Leishmania tarentolae 48 nm doublet microtubule protofilament ODADC2 focused refinement Method: EM (single particle) / Resolution: 3.6 Å EMDB-47651: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.3 focused refinement Method: EM (single particle) / Resolution: 3.3 Å 47661 9e78 EMDB-47661, PDB-9e78: 48-nm repeat of the Leishmania tarentolae doublet microtubule Method: EM (single particle) / Resolution: 2.9 Å EMDB-47680: Crithidia fasciculata doublet microtubule - ponticulus at position 1 within 48-nm repeat length Method: EM (single particle) / Resolution: 3.49 Å EMDB-47681: Crithidia fasciculata doublet microtubule - ponticulus at position 2 within 48-nm repeat length Method: EM (single particle) / Resolution: 3.58 Å EMDB-47682: Crithidia fasciculata doublet microtubule - ponticulus at position 3 within 48-nm repeat length Method: EM (single particle) / Resolution: 3.57 Å EMDB-47683: Crithidia fasciculata doublet microtubule - ODA-DC Method: EM (single particle) / Resolution: 3.27 Å EMDB-47684: Crithidia fasciculata doublet microtubule (48-nm periodicity) Method: EM (single particle) / Resolution: 3.1 Å EMDB-48302: Crithidia fasciculata doublet microtubule (consensus map, 48-nm periodicity) Method: EM (single particle) / Resolution: 3.1 Å
Chemicals	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM
Source	leishmania tarentolae (eukaryote) Crithidia fasciculata (eukaryote)
Keywords	STRUCTURAL PROTEIN / flagella / parasite / axoneme / doublet microtubule