EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Evolutionary adaptations of doublet microtubules in trypanosomatid parasites.
ジャーナル・号・ページ	Science, Vol. 387, Issue 6739, Page eadr5507, Year 2025
掲載日	2025年3月14日
著者	Matthew H Doran / Qingwei Niu / Jianwei Zeng / Tom Beneke / James Smith / Peter Ren / Sophia Fochler / Adrian Coscia / Johanna L Höög / Shimi Meleppattu / Polina V Lishko / Richard J Wheeler / Eva Gluenz / Rui Zhang / Alan Brown /
PubMed 要旨	The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum that contains an axoneme of dynein-bound doublet ...The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum that contains an axoneme of dynein-bound doublet microtubules (DMTs). In this work, we present cryo-electron microscopy structures of DMTs from two trypanosomatid species, and , at resolutions up to 2.7 angstrom. The structures revealed 27 trypanosomatid-specific microtubule inner proteins, a specialized dynein-docking complex, and the presence of paralogous proteins that enable higher-order periodicities or proximal-distal patterning. Leveraging the genetic tractability of trypanosomatid species, we quantified the location and contribution of each structure-identified protein to swimming behavior. Our study shows that proper B-tubule closure is critical for flagellar motility, exemplifying how integrating structural identification with systematic gene deletion can dissect individual protein contributions to flagellar motility.
リンク	Science / PubMed:40080577 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.6 Å
構造データ	EMDB-47607: Leishmania tarentolae 48 nm doublet microtubule consensus map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47608: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.1 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47609: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.2 focused refinement 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-47610: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.2 focused refinement 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-47612: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.1 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47613: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.2 focused refinement 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47614: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.3 focused refinement 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47615: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.1 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47616: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.2 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47618: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.3 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47619: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.1 focused refinement 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47620: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.2 focused refinement 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47622: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.3 focused refinement 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47624: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.1 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47627: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.2 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47630: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.3 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47633: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.1 focused refinement 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-47635: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.2 focused refinement 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-47636: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.3 focused refinement 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-47638: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.1 focused refinement 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-47639: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.2 focused refinement 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47640: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.1 focused refinement 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47641: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.2 focused refinement 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47642: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.3 focused refinement 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-47643: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.1 focused refinement 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47644: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.2 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47645: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.3 focused refinement 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47646: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.1 focused refinement 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47647: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.2 focused refinement 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47648: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.3 focused refinement 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47649: Leishmania tarentolae 48 nm doublet microtubule protofilament ODADC1 focused refinement 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-47650: Leishmania tarentolae 48 nm doublet microtubule protofilament ODADC2 focused refinement 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47651: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.3 focused refinement 手法: EM (単粒子) / 解像度: 3.3 Å 47661 9e78 EMDB-47661, PDB-9e78: 48-nm repeat of the Leishmania tarentolae doublet microtubule 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-47680: Crithidia fasciculata doublet microtubule - ponticulus at position 1 within 48-nm repeat length 手法: EM (単粒子) / 解像度: 3.49 Å EMDB-47681: Crithidia fasciculata doublet microtubule - ponticulus at position 2 within 48-nm repeat length 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-47682: Crithidia fasciculata doublet microtubule - ponticulus at position 3 within 48-nm repeat length 手法: EM (単粒子) / 解像度: 3.57 Å EMDB-47683: Crithidia fasciculata doublet microtubule - ODA-DC 手法: EM (単粒子) / 解像度: 3.27 Å EMDB-47684: Crithidia fasciculata doublet microtubule (48-nm periodicity) 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-48302: Crithidia fasciculata doublet microtubule (consensus map, 48-nm periodicity) 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, エネルギー貯蔵分子YM ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子*YM
由来	leishmania tarentolae (真核生物) Crithidia fasciculata (真核生物)
キーワード	STRUCTURAL PROTEIN / flagella / parasite / axoneme / doublet microtubule