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TitleArchitecture of the Sap S-layer of revealed by integrative structural biology.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 121, Issue 51, Page e2415351121, Year 2024
Publish dateDec 17, 2024
AuthorsAdrià Sogues / Kendra Leigh / Ethan V Halingstad / Sander E Van der Verren / Adam J Cecil / Antonella Fioravanti / Alexander J Pak / Misha Kudryashev / Han Remaut /
PubMed Abstract is a spore-forming gram-positive bacterium responsible for anthrax, an infectious disease with a high mortality rate and a target of concern due to bioterrorism and long-term site contamination. The ... is a spore-forming gram-positive bacterium responsible for anthrax, an infectious disease with a high mortality rate and a target of concern due to bioterrorism and long-term site contamination. The entire surface of vegetative cells in exponential or stationary growth phase is covered in proteinaceous arrays called S-layers, composed of Sap or EA1 protein, respectively. The Sap S-layer represents an important virulence factor and cell envelope support structure whose paracrystalline nature is essential for its function. However, the spatial organization of Sap in its lattice state remains elusive. Here, we employed cryoelectron tomography and subtomogram averaging to obtain a map of the Sap S-layer from tubular polymers that revealed a conformational switch between the postassembly protomers and the previously available X-ray structure of the condensed monomers. To build and validate an atomic model of the lattice within this map, we used a combination of molecular dynamics simulations, X-ray crystallography, cross-linking mass spectrometry, and biophysics in an integrative structural biology approach. The Sap lattice model produced recapitulates a close-to-physiological arrangement, reveals high-resolution details of lattice contacts, and sheds light on the mechanisms underlying the stability of the Sap layer.
External linksProc Natl Acad Sci U S A / PubMed:39652757 / PubMed Central
MethodsEM (subtomogram averaging) / X-ray diffraction
Resolution2.01 - 7.2 Å
Structure data

45459

9g93

EMDB-45459: Structure of in vitro assembled B. anthracis S-layer protein Sap
PDB-9g93: CryoET structure of the in vitro grown Bacillus anthracis Sap S-layer
Method: EM (subtomogram averaging) / Resolution: 7.2 Å

PDB-8rx2:
Domains 1 and 2 of Sap S-layer protein from Bacillus anthracis
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

PDB-8s80:
Domains 4 and 5 of Sap S-layer protein from Bacillus anthracis
Method: X-RAY DIFFRACTION / Resolution: 2.48 Å

PDB-8s83:
Domains 3 and 6 of Sap S-layer protein from Bacillus anthracis
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • bacillus anthracis (anthrax bacterium)
  • bacillus anthracis str. '34f2 (nmrc)' (bacteria)
KeywordsSTRUCTURAL PROTEIN / S-layer / Sap / Bacillus anthracis / anthracis / anthrax / exoskeleton / surface array

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