EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Nap1 and Kap114 co-chaperone H2A-H2B and facilitate targeted histone release in the nucleus.
ジャーナル・号・ページ	J Cell Biol, Vol. 224, Issue 1, Year 2025
掲載日	2025年1月6日
著者	Ho Yee Joyce Fung / Jenny Jiou / Ashley B Niesman / Natalia E Bernardes / Yuh Min Chook /
PubMed 要旨	Core histones, synthesized and processed in the cytoplasm, must be chaperoned as they are transported into the nucleus for nucleosome assembly. The importin Kap114 transports H2A-H2B into the yeast ...Core histones, synthesized and processed in the cytoplasm, must be chaperoned as they are transported into the nucleus for nucleosome assembly. The importin Kap114 transports H2A-H2B into the yeast nucleus, where RanGTP facilitates histone release. Kap114 and H2A-H2B also bind the histone chaperone Nap1, but how Nap1 and Kap114 cooperate in transport and nucleosome assembly remains unclear. Here, biochemical and structural analyses show that Kap114, H2A-H2B, and a Nap1 dimer (Nap12) associate in the absence and presence of RanGTP to form equimolar complexes. A previous study had shown that RanGTP reduces Kap114's ability to chaperone H2A-H2B, but a new cryo-EM structure of the Nap12•H2A-H2B•Kap114•RanGTP complex explains how both Kap114 and Nap12 interact with H2A-H2B, restoring its chaperoning within the assembly while effectively depositing it into nucleosomes. Together, our results suggest that Kap114 and Nap12 provide a sheltered path that facilitates the transfer of H2A-H2B from Kap114 to Nap12, ultimately directing its specific deposition into nucleosomes.
リンク	J Cell Biol / PubMed:39601790 / PubMed Central
手法	EM (単粒子)
解像度	2.88 - 5.62 Å
構造データ	44095 9b23 EMDB-44095, PDB-9b23: Cryo-EM structure of Nap1 core 手法: EM (単粒子) / 解像度: 3.2 Å 44120 9b31 EMDB-44120, PDB-9b31: Cryo-EM structure of yeast (Nap1)2-Kap114-H2A-H2B 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-44121: Local refined map for Nap1 region from (Nap1)2-Kap114-H2A-H2B structure 手法: EM (単粒子) / 解像度: 4.84 Å EMDB-44122: Consensus volume for (Nap1)2-Kap114-H2A-H2B 手法: EM (単粒子) / 解像度: 3.2 Å 44136 9b3f EMDB-44136, PDB-9b3f: Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114 手法: EM (単粒子) / 解像度: 3.54 Å EMDB-44137: Locally refined map for Nap1 and H2A-H2B in (Nap1)2-H2A-H2B-Kap114 structure 手法: EM (単粒子) / 解像度: 5.62 Å EMDB-44140: Consensus volume for (Nap1)2-H2A-H2B-Kap114 structure 手法: EM (単粒子) / 解像度: 3.54 Å 44141 9b3i EMDB-44141, PDB-9b3i: Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114-RanGTP 手法: EM (単粒子) / 解像度: 2.88 Å EMDB-44150: Local refined map for Nap1 and H2A-H2B region in (Nap1)2-H2A-H2B-Kap114-RanGTP structure 手法: EM (単粒子) / 解像度: 3.97 Å EMDB-44151: Consensus volume for (Nap1)2-H2A-H2B-Kap114-RanGTP 手法: EM (単粒子) / 解像度: 2.88 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM
由来	saccharomyces cerevisiae (パン酵母)
キーワード	CHAPERONE / Histone / TRANSPORT PROTEIN / Import / Nucleosome Assembly