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TitleIntracellular Ebola virus nucleocapsid assembly revealed by in situ cryo-electron tomography.
Journal, issue, pagesCell, Vol. 187, Issue 20, Page 5587-5603.e19, Year 2024
Publish dateOct 3, 2024
AuthorsReika Watanabe / Dawid Zyla / Diptiben Parekh / Connor Hong / Ying Jones / Sharon L Schendel / William Wan / Guillaume Castillon / Erica Ollmann Saphire /
PubMed AbstractFiloviruses, including the Ebola and Marburg viruses, cause hemorrhagic fevers with up to 90% lethality. The viral nucleocapsid is assembled by polymerization of the nucleoprotein (NP) along the ...Filoviruses, including the Ebola and Marburg viruses, cause hemorrhagic fevers with up to 90% lethality. The viral nucleocapsid is assembled by polymerization of the nucleoprotein (NP) along the viral genome, together with the viral proteins VP24 and VP35. We employed cryo-electron tomography of cells transfected with viral proteins and infected with model Ebola virus to illuminate assembly intermediates, as well as a 9 Å map of the complete intracellular assembly. This structure reveals a previously unresolved third and outer layer of NP complexed with VP35. The intrinsically disordered region, together with the C-terminal domain of this outer layer of NP, provides the constant width between intracellular nucleocapsid bundles and likely functions as a flexible tether to the viral matrix protein in the virion. A comparison of intracellular nucleocapsids with prior in-virion nucleocapsid structures reveals that the nucleocapsid further condenses vertically in the virion. The interfaces responsible for nucleocapsid assembly are highly conserved and offer targets for broadly effective antivirals.
External linksCell / PubMed:39293445 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution7.3 - 17.57 Å
Structure data

42509

8usn

EMDB-42509, PDB-8usn:
Intracellular cryo-tomography structure of EBOV nucleocapsid at 8.9 Angstrom
Method: EM (subtomogram averaging) / Resolution: 8.9 Å

EMDB-42515: Intracellular Ebola nucleocapsid-like structure obtained from cells expressing NP, VP24 and VP35 at 17.6 angstrom
Method: EM (subtomogram averaging) / Resolution: 17.57 Å

PDB-8ust:
In-virion structure of Ebola virus nucleocapsid-like assemblies from recombinant virus-like particles (nucleoprotein, VP24,VP35,VP40)
Method: ELECTRON MICROSCOPY / Resolution: 7.3 Å

Source
  • Homo sapiens (human)
  • Ebola virus - Mayinga, Zaire, 1976
  • zaire (virus)
  • ebola virus - mayinga
  • zaire, 1976
KeywordsVIRAL PROTEIN / nucleoprotein / nucleocapsid / Ebola virus / EBOV / filovirus / subtomogram averaging / cryo-ET / FIB / intracellular / in situ / VIRAL PROTEIN/RNA / VIRAL PROTEIN-RNA complex

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