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-Structure paper
Title | Structure and design of Langya virus glycoprotein antigens. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 121, Issue 16, Page e2314990121, Year 2024 |
Publish date | Apr 16, 2024 |
Authors | Zhaoqian Wang / Matthew McCallum / Lianying Yan / Cecily A Gibson / William Sharkey / Young-Jun Park / Ha V Dang / Moushimi Amaya / Ashley Person / Christopher C Broder / David Veesler / |
PubMed Abstract | Langya virus (LayV) is a recently discovered henipavirus (HNV), isolated from febrile patients in China. HNV entry into host cells is mediated by the attachment (G) and fusion (F) glycoproteins which ...Langya virus (LayV) is a recently discovered henipavirus (HNV), isolated from febrile patients in China. HNV entry into host cells is mediated by the attachment (G) and fusion (F) glycoproteins which are the main targets of neutralizing antibodies. We show here that the LayV F and G glycoproteins promote membrane fusion with human, mouse, and hamster target cells using a different, yet unknown, receptor than Nipah virus (NiV) and Hendra virus (HeV) and that NiV- and HeV-elicited monoclonal and polyclonal antibodies do not cross-react with LayV F and G. We determined cryoelectron microscopy structures of LayV F, in the prefusion and postfusion states, and of LayV G, revealing their conformational landscape and distinct antigenicity relative to NiV and HeV. We computationally designed stabilized LayV G constructs and demonstrate the generalizability of an HNV F prefusion-stabilization strategy. Our data will support the development of vaccines and therapeutics against LayV and closely related HNVs. |
External links | Proc Natl Acad Sci U S A / PubMed:38593070 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.8 - 3.9 Å |
Structure data | EMDB-41636, PDB-8tvb: EMDB-41639, PDB-8tve: EMDB-41640, PDB-8tvf: EMDB-41641, PDB-8tvg: EMDB-41642, PDB-8tvh: EMDB-41643, PDB-8tvi: EMDB-41644: Langya henipavirus postfusion fusion protein in complex with 4G5 Fab (global refinement) EMDB-43593, PDB-8vwp: |
Chemicals | ChemComp-NAG: ChemComp-HOH: ChemComp-ZN: |
Source |
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Keywords | VIRAL PROTEIN / GhV F / Glycoprotein / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / SSGCID / Inhibitor / VIRAL PROTEIN-IMMUNE SYSTEM complex / Langya / henipavirus / fusion protein / postfusion / LayVF / prefusion / VIRAL PROTEIN/IMMUNE SYSTEM / LayVG / LayV / G protein / attachment protein / attachment glycoprotein / Langya virus / protein design / protein stabilization / attachment |