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TitleCryoEM structure of a therapeutic antibody (favezelimab) bound to human LAG3 determined using a bivalent Fab as fiducial marker.
Journal, issue, pagesStructure, Vol. 31, Issue 10, Page 1149-11157.e3, Year 2023
Publish dateOct 5, 2023
AuthorsArjun K Mishra / Salman Shahid / Sharanbasappa S Karade / Pragati Agnihotri / Alexander Kolesnikov / S Saif Hasan / Roy A Mariuzza /
PubMed AbstractLymphocyte activation gene 3 protein (LAG3) is an inhibitory receptor that is upregulated on exhausted T cells in tumors. LAG3 is a major target for cancer immunotherapy with many anti-LAG3 ...Lymphocyte activation gene 3 protein (LAG3) is an inhibitory receptor that is upregulated on exhausted T cells in tumors. LAG3 is a major target for cancer immunotherapy with many anti-LAG3 antibodies in clinical trials. However, there is no structural information on the epitopes recognized by these antibodies. We determined the single-particle cryoEM structure of a therapeutic antibody (favezelimab) bound to LAG3 to 3.5 Å resolution, revealing that favezelimab targets the LAG3-binding site for MHC class II, its canonical ligand. The small size of the complex between the conventional (monovalent) Fab of favezelimab and LAG3 (∼100 kDa) presented a challenge for cryoEM. Accordingly, we engineered a bivalent version of Fab favezelimab that doubled the size of the Fab-LAG3 complex and conferred a highly identifiable shape to the complex that facilitated particle selection and orientation for image processing. This study establishes bivalent Fabs as new fiducial markers for cryoEM analysis of small proteins.
External linksStructure / PubMed:37619561
MethodsEM (single particle) / X-ray diffraction
Resolution2.1 - 3.61 Å
Structure data

40646

8so3

EMDB-40646, PDB-8so3:
CryoEM structure of a therapeutic antibody (favezelimab) bound to human LAG3
Method: EM (single particle) / Resolution: 3.61 Å

40716

8sr0

EMDB-40716, PDB-8sr0:
CryoEM structure of a therapeutic antibody (favezelimab) bound to human LAG3 local refined
Method: EM (single particle) / Resolution: 3.53 Å

PDB-6wkm:
Fab Fragment of Anti-human LAG3 antibody (22D2)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-8fwh:
Crystal structure of bivalent antibody Fab fragment of Anti-human LAG3 (22D2)
Method: X-RAY DIFFRACTION / Resolution: 2.833 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsIMMUNE SYSTEM / Anti Human LAG3 / Fab / inhibitory receptors / IMMUNOSUPPRESSANT / Lymphocyte activation gene 3 protein / favezelimab-LAG3 complex / cryoEM / Single particle reconstruction.

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