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-Structure paper
| タイトル | Cryo-EM Analysis of a Tri-Heme Cytochrome-Associated RC-LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae. |
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| ジャーナル・号・ページ | Adv Sci (Weinh), Vol. 12, Issue 18, Page e2413456, Year 2025 |
| 掲載日 | 2025年3月20日 |
著者 | Weiwei Wang / Yanting Liu / Jiayi Gu / Shaoya An / Cheng Ma / Haichun Gao / Nianzhi Jiao / Jian-Ren Shen / John Thomas Beatty / Michal Koblížek / Xing Zhang / Qiang Zheng / Jing-Hua Chen / ![]() |
| PubMed 要旨 | The reaction center-light harvesting 1 (RC-LH1) complex converts solar energy into electrical energy, driving the initiation of photosynthesis. The authors present a cryo-electron microscopy ...The reaction center-light harvesting 1 (RC-LH1) complex converts solar energy into electrical energy, driving the initiation of photosynthesis. The authors present a cryo-electron microscopy structure of the RC-LH1 isolated from a marine photoheterotrophic bacterium Dinoroseobacter shibae. The RC comprises four subunits, including a three-heme cytochrome (Cyt) c protein, and is surrounded by a closed LH ring composed of 17 pairs of antenna subunits. Notably, a novel subunit with an N-terminal "helix-turn-helix" motif embedded in the gap between the RC and the LH ring is identified. The purified RC-LH1 complex exhibits high stability in solutions containing Mg or Ca. The periplasmic Cyt c is predicted to bind at the junction between the Cyt subunit and the membrane plane, enabling electron transfer from Cyt c to the proximal heme of the tri-heme Cyt, and subsequently to the special pair of bacteriochlorophylls. These findings provide structural insights into the efficient energy and electron transfer processes within a distinct type of RC-LH1, and shed light on evolutionary adaptations of photosynthesis. |
リンク | Adv Sci (Weinh) / PubMed:40112203 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.68 - 2.78 Å |
| 構造データ | EMDB-39671, PDB-8yy9: EMDB-39683, PDB-8yz2: EMDB-62419, PDB-9km0: |
| 化合物 | ![]() ChemComp-BCL: ![]() PDB-1efu: ![]() ChemComp-LMT: ![]() ChemComp-MW9: ![]() ChemComp-FE: ![]() ChemComp-BPH: ![]() ChemComp-CDL: ![]() ChemComp-HEC: |
| 由来 |
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キーワード | PHOTOSYNTHESIS / Reaction center / Energy transfer / Photosynthetic bacteria |
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dinoroseobacter shibae dfl 12 = dsm 16493 (バクテリア)
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