Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Transcription regulation of African swine fever virus: dual role of M1249L.
Journal, issue, pages	Nat Commun, Vol. 15, Issue 1, Page 10058, Year 2024
Publish date	Nov 20, 2024
Authors	Dongming Zhao / Nan Wang / Xiaoying Feng / Zhenjiang Zhang / Kongen Xu / Tao Zheng / Yunge Yang / Xuemei Li / Xianjin Ou / Rui Zhao / Zihe Rao / Zhigao Bu / Yutao Chen / Xiangxi Wang /
PubMed Abstract	African swine fever virus (ASFV), which poses significant risks to the global economy, encodes a unique host-independent transcription system. This system comprises an eight-subunit RNA polymerase ...African swine fever virus (ASFV), which poses significant risks to the global economy, encodes a unique host-independent transcription system. This system comprises an eight-subunit RNA polymerase (vRNAP), temporally expressed transcription factors and transcript associated proteins, facilitating cross-species transmission via intermediate host. The protein composition of the virion and the presence of transcription factors in virus genome suggest existence of distinct transcription systems during viral infection. However, the precise mechanisms of transcription regulation remain elusive. Through analyses of dynamic transcriptome, vRNAP-associated components and cell-based assay, the critical role of M1249L in viral transcription regulation has been highlighted. Atomic-resolution structures of vRNAP-M1249L supercomplex, exhibiting a variety of conformations, have uncovered the dual functions of M1249L. During early transcription, M1249L could serve as multiple temporary transcription factors with C-terminal domain acting as a switcher for activation/inactivation, while during late transcription it aids in the packaging of the transcription machinery. The structural and functional characteristics of M1249L underscore its vital roles in ASFV transcription, packaging, and capsid assembly, presenting novel opportunities for therapeutic intervention.
External links	Nat Commun / PubMed:39567541 / PubMed Central
Methods	EM (single particle)
Resolution	2.56 - 9.32 Å
Structure data	39505 8yqt EMDB-39505, PDB-8yqt: African swine fever virus RNA Polymerase-M1249L complex2 Method: EM (single particle) / Resolution: 2.56 Å 39506 8yqu EMDB-39506, PDB-8yqu: African swine fever virus RNA Polymerase-M1249L complex1 Method: EM (single particle) / Resolution: 2.85 Å 39507 8yqv EMDB-39507, PDB-8yqv: African swine fever virus RNA Polymerase core Method: EM (single particle) / Resolution: 2.67 Å 39508 8yqw EMDB-39508, PDB-8yqw: ASFV RNA polymerase-M1249L complex3 Method: EM (single particle) / Resolution: 2.68 Å 39509 8yqx EMDB-39509, PDB-8yqx: ASFV RNA polymerase-M1249L complex4 Method: EM (single particle) / Resolution: 2.97 Å 39510 8yqy EMDB-39510, PDB-8yqy: ASFV RNA polymerase-M1249L complex complete Method: EM (single particle) / Resolution: 3.68 Å 39511 8yqz EMDB-39511, PDB-8yqz: African swine fever virus RNA Polymerase--DNA complex Method: EM (single particle) / Resolution: 2.78 Å EMDB-39512: ASFV RNA polymerase-M1249L complex 6 Method: EM (single particle) / Resolution: 9.23 Å EMDB-39513: ASFV RNA polymerase-M1249L complex 7 Method: EM (single particle) / Resolution: 5.17 Å EMDB-39514: ASFV RNA polymerase-M1249L complex 5 Method: EM (single particle) / Resolution: 3.23 Å EMDB-39515: ASFV RNA polymerase-M1249L complex 9 Method: EM (single particle) / Resolution: 4.04 Å EMDB-39516: ASFV RNA polymerase-M1249L complex 8 Method: EM (single particle) / Resolution: 3.19 Å EMDB-39517: ASFV RNA polymerase-M1249L complex10 Method: EM (single particle) / Resolution: 3.49 Å EMDB-39518: ASFV RNA polymerase-M1249L complex11 Method: EM (single particle) / Resolution: 4.17 Å EMDB-39519: ASFV RNA polymerase-M1249L complex 12 Method: EM (single particle) / Resolution: 3.8 Å EMDB-39520: ASFV RNA polymerase-M1249L complex14 Method: EM (single particle) / Resolution: 3.91 Å EMDB-39521: ASFV RNA polymerase-M1249L complex 13 Method: EM (single particle) / Resolution: 9.32 Å EMDB-39522: ASFV RNA polymerase-M1249L complex 15 Method: EM (single particle) / Resolution: 4.53 Å EMDB-39523: ASFV RNA polymerase-M1249L complex 16 Method: EM (single particle) / Resolution: 3.59 Å EMDB-39524: ASFV RNA polymerase-M1249L complex 17 Method: EM (single particle) / Resolution: 3.31 Å EMDB-39525: ASFV RNA polymerase-M1249L complex 18 Method: EM (single particle) / Resolution: 3.31 Å EMDB-39526: ASFV RNA polymerase-M1249L complex 19 Method: EM (single particle) / Resolution: 4.04 Å EMDB-39527: ASFV RNA polymerase-M1249L complex 20 Method: EM (single particle) / Resolution: 3.86 Å EMDB-39528: ASFV RNA polymerase-M1249L complex 21 Method: EM (single particle) / Resolution: 3.64 Å EMDB-39529: ASFV RNA polymerase-M1249L complex 24 Method: EM (single particle) / Resolution: 4.04 Å EMDB-39530: ASFV RNA polymerase-M1249L complex 22 Method: EM (single particle) / Resolution: 4.31 Å EMDB-39531: ASFV RNA polymerase-M1249L complex 23 Method: EM (single particle) / Resolution: 6.99 Å EMDB-39536: ASFV RNA polymerase-M1249L complex 25 Method: EM (single particle) / Resolution: 2.94 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry
Source	african swine fever virus
Keywords	VIRAL PROTEIN / ASFV / RNA polymerase / RNAP