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- EMDB-39510: ASFV RNA polymerase-M1249L complex complete -

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Basic information

Entry
Database: EMDB / ID: EMD-39510
TitleASFV RNA polymerase-M1249L complex complete
Map data
Sample
  • Complex: African swine fever virus RNA polymerase complex
    • Protein or peptide: x 9 types
  • Ligand: x 2 types
KeywordsASFV / RNA polymerase / VIRAL PROTEIN
Function / homology
Function and homology information


RNA polymerase III activity / viral transcription / RNA polymerase II activity / tRNA transcription by RNA polymerase III / RNA polymerase I activity / DNA-directed RNA polymerase complex / virion component / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase ...RNA polymerase III activity / viral transcription / RNA polymerase II activity / tRNA transcription by RNA polymerase III / RNA polymerase I activity / DNA-directed RNA polymerase complex / virion component / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / host cell cytoplasm / protein dimerization activity / DNA-templated transcription / DNA binding / zinc ion binding / metal ion binding / cytoplasm
Similarity search - Function
RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. ...RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerase, subunit H/Rpb5 C-terminal / RPB5-like RNA polymerase subunit superfamily / RNA polymerase Rpb5, C-terminal domain / Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site / RNA polymerases K / 14 to 18 Kd subunits signature. / RNA polymerase Rpb6 / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb1, domain 3 superfamily / RPB6/omega subunit-like superfamily / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, RBP11-like subunit / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6
Similarity search - Domain/homology
C122R / DNA-directed RNA polymerase RPB5 homolog / DNA-directed RNA polymerase RPB10 homolog / C147L / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase RPB3-11 homolog / D339L / M1249L / DNA-directed RNA polymerase subunit
Similarity search - Component
Biological speciesAfrican swine fever virus
Methodsingle particle reconstruction / cryo EM / Resolution: 3.68 Å
AuthorsFeng XY
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Regulation of early transcription and late transcript processing enzymes packaging for African swine fever virus from endogenous vRNAP-M1249L supercomplex structures
Authors: Feng XY
History
DepositionMar 20, 2024-
Header (metadata) releaseNov 6, 2024-
Map releaseNov 6, 2024-
UpdateNov 6, 2024-
Current statusNov 6, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_39510.png

Downloads & links

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Map

FileDownload / File: emd_39510.map.gz / Format: CCP4 / Size: 22.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.62 Å/pix.
x 180 pix.
= 290.88 Å		1.62 Å/pix.
x 180 pix.
= 290.88 Å		1.62 Å/pix.
x 180 pix.
= 290.88 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.616 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.018
Minimum - Maximum-0.055264607 - 0.09160972
Average (Standard dev.)-0.0000076595725 (±0.0052735903)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions180180180
Spacing180180180
CellA=B=C: 290.88 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_39510_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_39510_half_map_2.map
Projections & Slices
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Histogram

Histogram (log scale)

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Sample components

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Entire : African swine fever virus RNA polymerase complex

EntireName: African swine fever virus RNA polymerase complex
Components
  • Complex: African swine fever virus RNA polymerase complex
    • Protein or peptide: DNA-directed RNA polymerase subunit
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase RPB3-11 homolog
    • Protein or peptide: DNA-directed RNA polymerase RPB5 homolog
    • Protein or peptide: C147L
    • Protein or peptide: D339L
    • Protein or peptide: C122R
    • Protein or peptide: DNA-directed RNA polymerase RPB10 homolog
    • Protein or peptide: M1249L
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: African swine fever virus RNA polymerase complex

SupramoleculeName: African swine fever virus RNA polymerase complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#9
Source (natural)Organism: African swine fever virus

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Macromolecule #1: DNA-directed RNA polymerase subunit

MacromoleculeName: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 163.933938 KDa
SequenceString: MEAGYAEIAA VQFNIAGDND HKRQGVMEVT ISNLFEGTLP AEGGIYDARM GTTDHHYKCI TCSHQRKQCM GHPGILQMHA PVLQPLFIA EIRRWLRVIC LNCGAPIVDL KRYEHLIRPK RLIEAASSQT EGKQCYVCKA VHPKIVKDSE DYFTFWADQQ G KIDKLYPQ ...String:
MEAGYAEIAA VQFNIAGDND HKRQGVMEVT ISNLFEGTLP AEGGIYDARM GTTDHHYKCI TCSHQRKQCM GHPGILQMHA PVLQPLFIA EIRRWLRVIC LNCGAPIVDL KRYEHLIRPK RLIEAASSQT EGKQCYVCKA VHPKIVKDSE DYFTFWADQQ G KIDKLYPQ IIREIFSRVT YDTVVKLGRS KNSHPEKLVL KAIQIPPISI RPGIRLGIGS GPQSFHDINN VIQYLVRKNL LI PKDLQIV RGQKIPLNID RNLQTIQQLY YNFLLDSVST TATQGGTGKR GIVMGARPAP SIMRRLPRKE GRIRKSLLGS QVW SISRST ICGNSDLHLD EVGYPISFAR TLQVAETVQH YNINRLMPYF LNGKRQYPGC SRVYKQITQS VHDIEGLKQD FRLE VGDIL YRDVVTGDVA FFNRQPSLER SSIGVHRIVV LENPKISTFQ MNVSACAWYN ADFDGDQMNL WVPWSVMSRV EAELL CSVR NWFISTKSSG PVNGQVQDST VGSFLLTRTN TPMGKNVMNK LHAMGLFQTT QTDPPCFANY SPTDLLDGKS VVSMLL RQT PINYQRAPTW YSEVYAPYMH YNKQDISTQI RNGELIEGVL DKKAVGAGSS GGIYHLISRR YGPQQALKMI FATQQLA LN YVRNAGFTVS TADMLLTPEA HQEVQEIINE LLLESEEINN RLLHGDIMPP IGLTTHDFYE KLQLNALKFP DRILKPIM N SINPETNGLF QMVATGAKGS NPNMIHIMAG IGQIEINTQR IQPQFSFGRT LVYYPRFALE AQAYGFICNS YIAGLTSPE FIFGEMNGRF DLINKALSTS STGYANRKAI FGLQSCIVDY YRRVSIDTRL VQQLYGEDGL DARQLETVRF ETIMLSDQEL EDKFKYTGI QSPLFEEEFS RLKKDRDKYR QIFLNVENFN FSQLLTDVRQ VPVNVASIVK NILLSSTSGV LPFDEKSILQ K YAMVKTFC KNLPYVFINN IQERLQTPIP VYLKRAASLM RMLIRIELAT VKTLNITCEQ MSAILDLIRL QYTQSLINYG EA VGILAAQ SVSEPLTQYM LDSHHRSVAG GTNKSGIVRP QEIFSAKPVE AEQSSEMLLR LKNPEVETNK TYAQEIANSI ELI TFERLI LQWHLLYETY SSTKKNVMYP DFASDVEWMT DFLENHPLLQ PPEDIANWCI RLELNKTTMI LKSISLESII NSLR AKHPN TYIMHSVENT ASGIPIIIRI YLRESAFRRS TNTRMATDEK IAVNVVDKLL NSTIRGIPGI KNANVVKLMR HRVDA QGKL VRLDNIYAIK TNGTNIFGAM LDDNIDPYTI VSSSIGDTME LYGIEAARQK IISEIRTVMG DKGPNHRHLL MYADLM TRT GQVTSLEKAG LNAREPSNVL LRMALSSPVQ VLTDAAVDSA VNPIYGIAAP TLMGSVPRIG TMYSDIIMDE KYITENY KS VDSLIDML

UniProtKB: DNA-directed RNA polymerase subunit

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Macromolecule #2: DNA-directed RNA polymerase subunit beta

MacromoleculeName: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 140.056453 KDa
SequenceString: MEPLRPQITY GPIETVDNEE LTEADMLSFI SAAVNSTGLI GYNIKSFDDL MDNGIPQIVK QMFNVDITYK DQRDHTEIDK LRESVQIQF NFTDVNIERP QHRNYSQGNK INLLPNKARL CGLSYSGPVN LAAEVILTAH YSNGRQEVKR ASIPPFQVST F PIMRGSNR ...String:
MEPLRPQITY GPIETVDNEE LTEADMLSFI SAAVNSTGLI GYNIKSFDDL MDNGIPQIVK QMFNVDITYK DQRDHTEIDK LRESVQIQF NFTDVNIERP QHRNYSQGNK INLLPNKARL CGLSYSGPVN LAAEVILTAH YSNGRQEVKR ASIPPFQVST F PIMRGSNR CHTHHLSKTA KKEIGEDPNE PGGYFIARGG EWVVDLLENI RFNTLHIHYH TMQQGNNEII RGEFISQPGG AF ENSSQII IRYMTTGAIT IEINSTKFSK LRIPWYLIFR MFGMTGDDSI IEQVVFDLES NSLVNTFMIE ILEKSIHVLD PIF QPVQHE LNREKIIQFL SEKVSKFVSN PSAYKSDENA VQYLNERQLT ILDKILLPHM GQTADTRVRK LRFLGLLIHK ILLV IMNVF PPTDRDSYRT KRVHGSGVSL AKAFKAIFNT SVIAPIINGF KELLKQTAFE ELTQRNIIEA FSAALSKNTA SDLNR SMEQ SIISGNKTIM VRQRPIVNRV STQSLERKNL LNTISALRTV NTHNTTNASK QTERADMMRR VHASYPGYIC VAQSAD TGE KVGMSKQLAI TANVCTAGEV LSLKQRLLSD PAIQQLADVS NKDIVRKGLA RVFINGEWIG CCTNAFELAQ RYRMLRR EG KVVHPHTTIY WDSMVDEVEF WLDVGRLTRP LLIVDNNIEK YNQACYKAAE ARKKGDKDWE KHKIPFIQNT RFTPQMAK D ILAGTLTLED LVAQGICEFI TPEEAENCLV AFSIIELRKH KHDVTRRFTH VDVPQAILGL AALVSPYANC TQPARVTYE TNQGRQTGGW YCFSWPYRVD MNRFFQFYNE MPLVKTIAHN YVIPNGLNTI VAYMIYGGYN QEDSVIVSQS FIDRGGFAGT FYREEKVEL ESDIESFGKP DPLITKNLKP GANYEKLVDG FVPVGTVVKK GDIIIGKVAK IRGEKDELNK YIDRSVMYGF D EPAVVDAV MRPHGPNDEI FGLMRLRYER NLNIGDKMSS RSGNKGIAAL ALPTSDMPFT EDGLQPDLIV NPHSHPSRMT NG QMIETTV GLANALQGVV TDGTAFLPIN VQLLSERLAQ EGLRFNGCQK MFNGQTGEYF DAAIFIGPTY HQRLQKFVLD DRY AVASYG PTDALTGQPL DGKRSHGGLR LGEMEHWVLT AQGAMQTIIE KSHDDSDGCI SYICRNCGEP AIYNASHPIY KCMN CDVQA DIGMVDSRRS SIVFQHEMRA ANVNITSVLS PRVFQPA

UniProtKB: DNA-directed RNA polymerase subunit beta

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Macromolecule #3: DNA-directed RNA polymerase RPB3-11 homolog

MacromoleculeName: DNA-directed RNA polymerase RPB3-11 homolog / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 41.418137 KDa
SequenceString: MEKIFQNVEI KPFLIDFSNL FIKNAAKKLF QLEEQLPLVP VNVVMDFKGI SRAAVHGLSR VLQDEIPNYM LDIKPGGYKI EDSTDLFMT EQFIRNRINF IPIYAKNETL VFALRSLNNS CEVKTIYSRD LIQVAGPKLK YPIFNPTFEI GFLQPGKSLI I EDIYIKKG ...String:
MEKIFQNVEI KPFLIDFSNL FIKNAAKKLF QLEEQLPLVP VNVVMDFKGI SRAAVHGLSR VLQDEIPNYM LDIKPGGYKI EDSTDLFMT EQFIRNRINF IPIYAKNETL VFALRSLNNS CEVKTIYSRD LIQVAGPKLK YPIFNPTFEI GFLQPGKSLI I EDIYIKKG IGRKHAAFNL AVKTHFSHLD IEQYPTDKKE YMALSGYKQS SMTSDPRHHR LGLCFPAVPL PHINQAVRTY LK NACRIII GRIQSIQKIY ENFEEPQPEL VLFSMDEEKT KAIITIKDET HTIGNLLKTY IYEMIPDISF VGYQCVPHKQ EMV LTIIHK ASQEDLITLL EKSIQNIIQT FQILEKNVDE LIA

UniProtKB: DNA-directed RNA polymerase RPB3-11 homolog

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Macromolecule #4: DNA-directed RNA polymerase RPB5 homolog

MacromoleculeName: DNA-directed RNA polymerase RPB5 homolog / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 23.693711 KDa
SequenceString: MAMQKLFTYI YEFIEYRKMV LLEEKVPYDK FVQMVLNTGF FRINAETLNH GIVSVFIFGA NGKYVHHGGD MRTLLTNTLN EKKHYEELI LIVDKPVLSK KNILDIIVEQ RAANPTIVIN IYPYHLFCIN IPKVSAIPKH KLITQEEAQE FLGREYLQPQ D LMQISASD ...String:
MAMQKLFTYI YEFIEYRKMV LLEEKVPYDK FVQMVLNTGF FRINAETLNH GIVSVFIFGA NGKYVHHGGD MRTLLTNTLN EKKHYEELI LIVDKPVLSK KNILDIIVEQ RAANPTIVIN IYPYHLFCIN IPKVSAIPKH KLITQEEAQE FLGREYLQPQ D LMQISASD PPVVWLGGRP GDFVQIERPS ETAMHAVVIR FITKSKI

UniProtKB: DNA-directed RNA polymerase RPB5 homolog

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Macromolecule #5: C147L

MacromoleculeName: C147L / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 16.670795 KDa
SequenceString:
MADNDNEDLI MDDLVEEYVE TEEENLVDSE EESEDKDEIV ESPSICEGFV QASSQTLVII PDNERITSNV LTTFEATRLV AVRAQQLAI NGSTMLKKKY SSPIDIAKQE LFNRKIPLLV MRCIKVTPEG QKIVEIWNPR EMGIPLLD

UniProtKB: C147L

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Macromolecule #6: D339L

MacromoleculeName: D339L / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 38.798594 KDa
SequenceString: MIDQKIFETT LNIDDPTNFC TNVEAHLLKE LENIYVGKCF KNSFILNITG VIQRSPCFIM RTNNSGRGYM HVRFSAVVSY LNAFDLIAA VKIIKNDSNI ILGESLLTEP VTIVIPSSES QNNVAEVGQI VPVQLANSSV YYIPGRQQAS ATGSIFIPKH T FSVYHVQE ...String:
MIDQKIFETT LNIDDPTNFC TNVEAHLLKE LENIYVGKCF KNSFILNITG VIQRSPCFIM RTNNSGRGYM HVRFSAVVSY LNAFDLIAA VKIIKNDSNI ILGESLLTEP VTIVIPSSES QNNVAEVGQI VPVQLANSSV YYIPGRQQAS ATGSIFIPKH T FSVYHVQE ELTQEQALNL TKLVNIIEML LESRSKKDFK QICFFEKLYY TYSISSDEIL DLKIWKGPKG KEMSRLKPCN VL SFLYDAL KNKNSSLGFW ARPPNLLKSS PLAYQQDQNS FNATELPIIC SAEVMFVTLL KEIINYLQFI NDLCDTFNNE QLI KRHENI WMLIEQRKIG HDF

UniProtKB: D339L

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Macromolecule #7: C122R

MacromoleculeName: C122R / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 11.831861 KDa
SequenceString:
MKICKACSSC MVRTYVDGNI IFRCSCGESV QGDSQNLLVS SKVYHTGEME DKYKIFIKNA PFDPTNCQIK KDCPNCHLDY LTQICIGSQ KIIILVCRCG YMSNRG

UniProtKB: C122R

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Macromolecule #8: DNA-directed RNA polymerase RPB10 homolog

MacromoleculeName: DNA-directed RNA polymerase RPB10 homolog / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 9.04079 KDa
SequenceString:
MLIPVVCFTC GFPIGTYAAI FDKARTEYIK TKMGGTLPQN IPLDASLQIE LKDLITALGI PMRVCCRTHL ITTLDYRKYY

UniProtKB: DNA-directed RNA polymerase RPB10 homolog

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Macromolecule #9: M1249L

MacromoleculeName: M1249L / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 144.604125 KDa
SequenceString: MEEVITIAQI VHRGTDILSL NNEEIEALVD EIYSTLKGSN DIKNIRLIDF LFTLKDFVNH VRAEQSKLPD LSMPIEAYIR QLLVDPDVV PIVSEKKKEL RVRPSTRKEI FLINGTHLAV PAEAPIEIYG LKLRLKTFSP QCFMRMAEIG SFSPETLGYV A SGANLTNF ...String:
MEEVITIAQI VHRGTDILSL NNEEIEALVD EIYSTLKGSN DIKNIRLIDF LFTLKDFVNH VRAEQSKLPD LSMPIEAYIR QLLVDPDVV PIVSEKKKEL RVRPSTRKEI FLINGTHLAV PAEAPIEIYG LKLRLKTFSP QCFMRMAEIG SFSPETLGYV A SGANLTNF IRVFMKCVDQ ETWKKNGEGV VVTTKENIIQ FTHQYIELYK FLRSGGHSWL INRLAEEMVH RKLDREDQGS HI SNIVETE EIEPEENIKR VIFFLKELST MYSVSPVFTS GYMPLLYDLY RAGYLEVLWN PVEQKFLQHA EQREKEQMIL QQV DMKLTE VITQARQYFK IMEEKIGRVQ SDAIREILTM EGKVDDPNSI LQEVIKACGK QEAELITTEY LNIKKQWELQ EKNA CAHLK LVKQLRSGLQ YAELLKVLES IRVLYKEKNN TTNWNLCKAC GFKLLCPHVD MLIQLQAAEA SYDTMRTKLM KFSGI NKEK ENNQGLIYSY FCKICGEELA HFIQEDRTAD VGIIGDLNSK LRVFIWQETM KACTFIHFGK LVDVKQFANI AVNVCL PLV YSIENIKKEE DYDPLTQLYA VIYIYAYILN LIYSSQKNKE FLTITIHGMK ADSSLNAYVT FLLEKMMQQY SGIINQL SE ITDQWIANNF REAFKKIIHQ NGLQGLSVQD DTKVLLTEIL LDPMYDYAAT VARIDGSIPM HKPRTPKEAE YEFKTVIG R TPAELLSQKE FYDKIYTSKY RPDFTQLTRL NDIYFQEESL RVWWGGRDEE KTSTLIYLRA YELFLKYLQN APNFNSELA EFKTYENAYG EQKALLAQQG FYNIFDPNTG RADQRTRLFE YKRLPISTLY DERGLPHKWT IYVYKAVDSS QKPAEIEVTR KDVIKKIDN HYALADLRCS VCHVLQHEVG QLNIKKVQTA LKASLEFNTF YAFYESRCPK GGLHDFQDKK CVKCGLFTYI I YDHLSQPE LVHDYYNNYK DQYDKEKMSI RSIQIKKDMT TPSTETQPKP PQEPWTFDYG KIIKTAKILD ISPAVIEAIG AM EGRSYAD IREGQGAPPP PTSMDDPRLM AVDSAVRIFL YNYNCLRHVS TFNKPPIHVE RLVKHLSYEE KEDLEKVLPN VVN EYHTTF KHLRVTDPAS ALLYSIEFLC ISFLTLYEIK EPSWVVNIVR EFALTELNTI IQSEKLLSKP GAFNFMIFGE DFVC SGEDS SMDDISAYSS PGLFGEDIID RLDDPFSIED VDISLDVLDN LAPQ

UniProtKB: M1249L

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Macromolecule #10: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 10 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #11: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 11 / Number of copies: 9 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 %

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 1.25 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.68 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 10433
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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