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- EMDB-39511: African swine fever virus RNA Polymerase--DNA complex -

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Basic information

Entry
Database: EMDB / ID: EMD-39511
TitleAfrican swine fever virus RNA Polymerase--DNA complex
Map data
Sample
  • Complex: African swine fever virus RNA polymerase complex
    • Protein or peptide: x 8 types
    • DNA: x 2 types
  • Ligand: x 2 types
KeywordsASFV / RNA polymerase / VIRAL PROTEIN
Function / homology
Function and homology information


viral transcription / transcription elongation by RNA polymerase I / tRNA transcription by RNA polymerase III / DNA-directed RNA polymerase activity / DNA-directed RNA polymerase complex / ribonucleoside binding / virion component / : / : / : ...viral transcription / transcription elongation by RNA polymerase I / tRNA transcription by RNA polymerase III / DNA-directed RNA polymerase activity / DNA-directed RNA polymerase complex / ribonucleoside binding / virion component / : / : / : / : / : / : / DNA-directed RNA polymerase / host cell cytoplasm / protein dimerization activity / DNA-templated transcription / DNA binding / zinc ion binding
Similarity search - Function
RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. ...RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerase, subunit H/Rpb5 C-terminal / DNA-directed RNA polymerase subunit Rpo5/Rpb5 / RPB5-like RNA polymerase subunit superfamily / RNA polymerase Rpb5, C-terminal domain / Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site / RNA polymerases K / 14 to 18 Kd subunits signature. / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb6 / RNA polymerase, subunit omega/Rpo6/RPB6 / RNA polymerase Rpb6 / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase Rpb1, domain 3 superfamily / RPB6/omega subunit-like superfamily / RNA polymerase Rpb1, clamp domain superfamily / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 4 / DNA-directed RNA polymerase, insert domain superfamily / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, RBP11-like subunit / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6
Similarity search - Domain/homology
C122R / DNA-directed RNA polymerase RPB5 homolog / DNA-directed RNA polymerase RPB10 homolog / C147L / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase RPB3-11 homolog / D339L / DNA-directed RNA polymerase subunit
Similarity search - Component
Biological speciesAfrican swine fever virus
Methodsingle particle reconstruction / cryo EM / Resolution: 2.78 Å
AuthorsFeng XY
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Nat Commun / Year: 2024
Title: Transcription regulation of African swine fever virus: dual role of M1249L.
Authors: Dongming Zhao / Nan Wang / Xiaoying Feng / Zhenjiang Zhang / Kongen Xu / Tao Zheng / Yunge Yang / Xuemei Li / Xianjin Ou / Rui Zhao / Zihe Rao / Zhigao Bu / Yutao Chen / Xiangxi Wang /
Abstract: African swine fever virus (ASFV), which poses significant risks to the global economy, encodes a unique host-independent transcription system. This system comprises an eight-subunit RNA polymerase ...African swine fever virus (ASFV), which poses significant risks to the global economy, encodes a unique host-independent transcription system. This system comprises an eight-subunit RNA polymerase (vRNAP), temporally expressed transcription factors and transcript associated proteins, facilitating cross-species transmission via intermediate host. The protein composition of the virion and the presence of transcription factors in virus genome suggest existence of distinct transcription systems during viral infection. However, the precise mechanisms of transcription regulation remain elusive. Through analyses of dynamic transcriptome, vRNAP-associated components and cell-based assay, the critical role of M1249L in viral transcription regulation has been highlighted. Atomic-resolution structures of vRNAP-M1249L supercomplex, exhibiting a variety of conformations, have uncovered the dual functions of M1249L. During early transcription, M1249L could serve as multiple temporary transcription factors with C-terminal domain acting as a switcher for activation/inactivation, while during late transcription it aids in the packaging of the transcription machinery. The structural and functional characteristics of M1249L underscore its vital roles in ASFV transcription, packaging, and capsid assembly, presenting novel opportunities for therapeutic intervention.
History
DepositionMar 20, 2024-
Header (metadata) releaseNov 6, 2024-
Map releaseNov 6, 2024-
UpdateJun 11, 2025-
Current statusJun 11, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_39511.png

Downloads & links

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Map

FileDownload / File: emd_39511.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.81 Å/pix.
x 320 pix.
= 258.56 Å		0.81 Å/pix.
x 320 pix.
= 258.56 Å		0.81 Å/pix.
x 320 pix.
= 258.56 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.808 Å
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Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0129
Minimum - Maximum-0.0895382 - 0.14796455
Average (Standard dev.)-0.0001035885 (±0.0046128198)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 258.56 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_39511_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_39511_half_map_2.map
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Histogram (log scale)

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Sample components

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Entire : African swine fever virus RNA polymerase complex

EntireName: African swine fever virus RNA polymerase complex
Components
  • Complex: African swine fever virus RNA polymerase complex
    • Protein or peptide: DNA-directed RNA polymerase subunit
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase RPB3-11 homolog
    • Protein or peptide: DNA-directed RNA polymerase RPB5 homolog
    • Protein or peptide: C147L
    • Protein or peptide: D339L
    • Protein or peptide: C122R
    • Protein or peptide: DNA-directed RNA polymerase RPB10 homolog
    • DNA: DNA (5'-D(P*GP*CP*CP*GP*AP*GP*CP*A)-3')
    • DNA: DNA (5'-D(P*TP*CP*GP*GP*CP*TP*C)-3')
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: African swine fever virus RNA polymerase complex

SupramoleculeName: African swine fever virus RNA polymerase complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#10
Source (natural)Organism: African swine fever virus

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Macromolecule #1: DNA-directed RNA polymerase subunit

MacromoleculeName: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 163.933938 KDa
SequenceString: MEAGYAEIAA VQFNIAGDND HKRQGVMEVT ISNLFEGTLP AEGGIYDARM GTTDHHYKCI TCSHQRKQCM GHPGILQMHA PVLQPLFIA EIRRWLRVIC LNCGAPIVDL KRYEHLIRPK RLIEAASSQT EGKQCYVCKA VHPKIVKDSE DYFTFWADQQ G KIDKLYPQ ...String:
MEAGYAEIAA VQFNIAGDND HKRQGVMEVT ISNLFEGTLP AEGGIYDARM GTTDHHYKCI TCSHQRKQCM GHPGILQMHA PVLQPLFIA EIRRWLRVIC LNCGAPIVDL KRYEHLIRPK RLIEAASSQT EGKQCYVCKA VHPKIVKDSE DYFTFWADQQ G KIDKLYPQ IIREIFSRVT YDTVVKLGRS KNSHPEKLVL KAIQIPPISI RPGIRLGIGS GPQSFHDINN VIQYLVRKNL LI PKDLQIV RGQKIPLNID RNLQTIQQLY YNFLLDSVST TATQGGTGKR GIVMGARPAP SIMRRLPRKE GRIRKSLLGS QVW SISRST ICGNSDLHLD EVGYPISFAR TLQVAETVQH YNINRLMPYF LNGKRQYPGC SRVYKQITQS VHDIEGLKQD FRLE VGDIL YRDVVTGDVA FFNRQPSLER SSIGVHRIVV LENPKISTFQ MNVSACAWYN ADFDGDQMNL WVPWSVMSRV EAELL CSVR NWFISTKSSG PVNGQVQDST VGSFLLTRTN TPMGKNVMNK LHAMGLFQTT QTDPPCFANY SPTDLLDGKS VVSMLL RQT PINYQRAPTW YSEVYAPYMH YNKQDISTQI RNGELIEGVL DKKAVGAGSS GGIYHLISRR YGPQQALKMI FATQQLA LN YVRNAGFTVS TADMLLTPEA HQEVQEIINE LLLESEEINN RLLHGDIMPP IGLTTHDFYE KLQLNALKFP DRILKPIM N SINPETNGLF QMVATGAKGS NPNMIHIMAG IGQIEINTQR IQPQFSFGRT LVYYPRFALE AQAYGFICNS YIAGLTSPE FIFGEMNGRF DLINKALSTS STGYANRKAI FGLQSCIVDY YRRVSIDTRL VQQLYGEDGL DARQLETVRF ETIMLSDQEL EDKFKYTGI QSPLFEEEFS RLKKDRDKYR QIFLNVENFN FSQLLTDVRQ VPVNVASIVK NILLSSTSGV LPFDEKSILQ K YAMVKTFC KNLPYVFINN IQERLQTPIP VYLKRAASLM RMLIRIELAT VKTLNITCEQ MSAILDLIRL QYTQSLINYG EA VGILAAQ SVSEPLTQYM LDSHHRSVAG GTNKSGIVRP QEIFSAKPVE AEQSSEMLLR LKNPEVETNK TYAQEIANSI ELI TFERLI LQWHLLYETY SSTKKNVMYP DFASDVEWMT DFLENHPLLQ PPEDIANWCI RLELNKTTMI LKSISLESII NSLR AKHPN TYIMHSVENT ASGIPIIIRI YLRESAFRRS TNTRMATDEK IAVNVVDKLL NSTIRGIPGI KNANVVKLMR HRVDA QGKL VRLDNIYAIK TNGTNIFGAM LDDNIDPYTI VSSSIGDTME LYGIEAARQK IISEIRTVMG DKGPNHRHLL MYADLM TRT GQVTSLEKAG LNAREPSNVL LRMALSSPVQ VLTDAAVDSA VNPIYGIAAP TLMGSVPRIG TMYSDIIMDE KYITENY KS VDSLIDML

UniProtKB: DNA-directed RNA polymerase subunit

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Macromolecule #2: DNA-directed RNA polymerase subunit beta

MacromoleculeName: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 140.056453 KDa
SequenceString: MEPLRPQITY GPIETVDNEE LTEADMLSFI SAAVNSTGLI GYNIKSFDDL MDNGIPQIVK QMFNVDITYK DQRDHTEIDK LRESVQIQF NFTDVNIERP QHRNYSQGNK INLLPNKARL CGLSYSGPVN LAAEVILTAH YSNGRQEVKR ASIPPFQVST F PIMRGSNR ...String:
MEPLRPQITY GPIETVDNEE LTEADMLSFI SAAVNSTGLI GYNIKSFDDL MDNGIPQIVK QMFNVDITYK DQRDHTEIDK LRESVQIQF NFTDVNIERP QHRNYSQGNK INLLPNKARL CGLSYSGPVN LAAEVILTAH YSNGRQEVKR ASIPPFQVST F PIMRGSNR CHTHHLSKTA KKEIGEDPNE PGGYFIARGG EWVVDLLENI RFNTLHIHYH TMQQGNNEII RGEFISQPGG AF ENSSQII IRYMTTGAIT IEINSTKFSK LRIPWYLIFR MFGMTGDDSI IEQVVFDLES NSLVNTFMIE ILEKSIHVLD PIF QPVQHE LNREKIIQFL SEKVSKFVSN PSAYKSDENA VQYLNERQLT ILDKILLPHM GQTADTRVRK LRFLGLLIHK ILLV IMNVF PPTDRDSYRT KRVHGSGVSL AKAFKAIFNT SVIAPIINGF KELLKQTAFE ELTQRNIIEA FSAALSKNTA SDLNR SMEQ SIISGNKTIM VRQRPIVNRV STQSLERKNL LNTISALRTV NTHNTTNASK QTERADMMRR VHASYPGYIC VAQSAD TGE KVGMSKQLAI TANVCTAGEV LSLKQRLLSD PAIQQLADVS NKDIVRKGLA RVFINGEWIG CCTNAFELAQ RYRMLRR EG KVVHPHTTIY WDSMVDEVEF WLDVGRLTRP LLIVDNNIEK YNQACYKAAE ARKKGDKDWE KHKIPFIQNT RFTPQMAK D ILAGTLTLED LVAQGICEFI TPEEAENCLV AFSIIELRKH KHDVTRRFTH VDVPQAILGL AALVSPYANC TQPARVTYE TNQGRQTGGW YCFSWPYRVD MNRFFQFYNE MPLVKTIAHN YVIPNGLNTI VAYMIYGGYN QEDSVIVSQS FIDRGGFAGT FYREEKVEL ESDIESFGKP DPLITKNLKP GANYEKLVDG FVPVGTVVKK GDIIIGKVAK IRGEKDELNK YIDRSVMYGF D EPAVVDAV MRPHGPNDEI FGLMRLRYER NLNIGDKMSS RSGNKGIAAL ALPTSDMPFT EDGLQPDLIV NPHSHPSRMT NG QMIETTV GLANALQGVV TDGTAFLPIN VQLLSERLAQ EGLRFNGCQK MFNGQTGEYF DAAIFIGPTY HQRLQKFVLD DRY AVASYG PTDALTGQPL DGKRSHGGLR LGEMEHWVLT AQGAMQTIIE KSHDDSDGCI SYICRNCGEP AIYNASHPIY KCMN CDVQA DIGMVDSRRS SIVFQHEMRA ANVNITSVLS PRVFQPA

UniProtKB: DNA-directed RNA polymerase subunit beta

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Macromolecule #3: DNA-directed RNA polymerase RPB3-11 homolog

MacromoleculeName: DNA-directed RNA polymerase RPB3-11 homolog / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 41.418137 KDa
SequenceString: MEKIFQNVEI KPFLIDFSNL FIKNAAKKLF QLEEQLPLVP VNVVMDFKGI SRAAVHGLSR VLQDEIPNYM LDIKPGGYKI EDSTDLFMT EQFIRNRINF IPIYAKNETL VFALRSLNNS CEVKTIYSRD LIQVAGPKLK YPIFNPTFEI GFLQPGKSLI I EDIYIKKG ...String:
MEKIFQNVEI KPFLIDFSNL FIKNAAKKLF QLEEQLPLVP VNVVMDFKGI SRAAVHGLSR VLQDEIPNYM LDIKPGGYKI EDSTDLFMT EQFIRNRINF IPIYAKNETL VFALRSLNNS CEVKTIYSRD LIQVAGPKLK YPIFNPTFEI GFLQPGKSLI I EDIYIKKG IGRKHAAFNL AVKTHFSHLD IEQYPTDKKE YMALSGYKQS SMTSDPRHHR LGLCFPAVPL PHINQAVRTY LK NACRIII GRIQSIQKIY ENFEEPQPEL VLFSMDEEKT KAIITIKDET HTIGNLLKTY IYEMIPDISF VGYQCVPHKQ EMV LTIIHK ASQEDLITLL EKSIQNIIQT FQILEKNVDE LIA

UniProtKB: DNA-directed RNA polymerase RPB3-11 homolog

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Macromolecule #4: DNA-directed RNA polymerase RPB5 homolog

MacromoleculeName: DNA-directed RNA polymerase RPB5 homolog / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 23.693711 KDa
SequenceString: MAMQKLFTYI YEFIEYRKMV LLEEKVPYDK FVQMVLNTGF FRINAETLNH GIVSVFIFGA NGKYVHHGGD MRTLLTNTLN EKKHYEELI LIVDKPVLSK KNILDIIVEQ RAANPTIVIN IYPYHLFCIN IPKVSAIPKH KLITQEEAQE FLGREYLQPQ D LMQISASD ...String:
MAMQKLFTYI YEFIEYRKMV LLEEKVPYDK FVQMVLNTGF FRINAETLNH GIVSVFIFGA NGKYVHHGGD MRTLLTNTLN EKKHYEELI LIVDKPVLSK KNILDIIVEQ RAANPTIVIN IYPYHLFCIN IPKVSAIPKH KLITQEEAQE FLGREYLQPQ D LMQISASD PPVVWLGGRP GDFVQIERPS ETAMHAVVIR FITKSKI

UniProtKB: DNA-directed RNA polymerase RPB5 homolog

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Macromolecule #5: C147L

MacromoleculeName: C147L / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 16.670795 KDa
SequenceString:
MADNDNEDLI MDDLVEEYVE TEEENLVDSE EESEDKDEIV ESPSICEGFV QASSQTLVII PDNERITSNV LTTFEATRLV AVRAQQLAI NGSTMLKKKY SSPIDIAKQE LFNRKIPLLV MRCIKVTPEG QKIVEIWNPR EMGIPLLD

UniProtKB: C147L

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Macromolecule #6: D339L

MacromoleculeName: D339L / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 38.798594 KDa
SequenceString: MIDQKIFETT LNIDDPTNFC TNVEAHLLKE LENIYVGKCF KNSFILNITG VIQRSPCFIM RTNNSGRGYM HVRFSAVVSY LNAFDLIAA VKIIKNDSNI ILGESLLTEP VTIVIPSSES QNNVAEVGQI VPVQLANSSV YYIPGRQQAS ATGSIFIPKH T FSVYHVQE ...String:
MIDQKIFETT LNIDDPTNFC TNVEAHLLKE LENIYVGKCF KNSFILNITG VIQRSPCFIM RTNNSGRGYM HVRFSAVVSY LNAFDLIAA VKIIKNDSNI ILGESLLTEP VTIVIPSSES QNNVAEVGQI VPVQLANSSV YYIPGRQQAS ATGSIFIPKH T FSVYHVQE ELTQEQALNL TKLVNIIEML LESRSKKDFK QICFFEKLYY TYSISSDEIL DLKIWKGPKG KEMSRLKPCN VL SFLYDAL KNKNSSLGFW ARPPNLLKSS PLAYQQDQNS FNATELPIIC SAEVMFVTLL KEIINYLQFI NDLCDTFNNE QLI KRHENI WMLIEQRKIG HDF

UniProtKB: D339L

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Macromolecule #7: C122R

MacromoleculeName: C122R / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 11.831861 KDa
SequenceString:
MKICKACSSC MVRTYVDGNI IFRCSCGESV QGDSQNLLVS SKVYHTGEME DKYKIFIKNA PFDPTNCQIK KDCPNCHLDY LTQICIGSQ KIIILVCRCG YMSNRG

UniProtKB: C122R

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Macromolecule #8: DNA-directed RNA polymerase RPB10 homolog

MacromoleculeName: DNA-directed RNA polymerase RPB10 homolog / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 9.04079 KDa
SequenceString:
MLIPVVCFTC GFPIGTYAAI FDKARTEYIK TKMGGTLPQN IPLDASLQIE LKDLITALGI PMRVCCRTHL ITTLDYRKYY

UniProtKB: DNA-directed RNA polymerase RPB10 homolog

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Macromolecule #9: DNA (5'-D(P*GP*CP*CP*GP*AP*GP*CP*A)-3')

MacromoleculeName: DNA (5'-D(P*GP*CP*CP*GP*AP*GP*CP*A)-3') / type: dna / ID: 9 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 2.436619 KDa
SequenceString:
(DG)(DC)(DC)(DG)(DA)(DG)(DC)(DA)

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Macromolecule #10: DNA (5'-D(P*TP*CP*GP*GP*CP*TP*C)-3')

MacromoleculeName: DNA (5'-D(P*TP*CP*GP*GP*CP*TP*C)-3') / type: dna / ID: 10 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: African swine fever virus
Molecular weightTheoretical: 2.089385 KDa
SequenceString:
(DT)(DC)(DG)(DG)(DC)(DT)(DC)

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Macromolecule #11: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 11 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #12: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 12 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 %

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 1.25 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.78 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 266388
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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