EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Transcription regulation of African swine fever virus: dual role of M1249L.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 10058, Year 2024
掲載日	2024年11月20日
著者	Dongming Zhao / Nan Wang / Xiaoying Feng / Zhenjiang Zhang / Kongen Xu / Tao Zheng / Yunge Yang / Xuemei Li / Xianjin Ou / Rui Zhao / Zihe Rao / Zhigao Bu / Yutao Chen / Xiangxi Wang /
PubMed 要旨	African swine fever virus (ASFV), which poses significant risks to the global economy, encodes a unique host-independent transcription system. This system comprises an eight-subunit RNA polymerase ...African swine fever virus (ASFV), which poses significant risks to the global economy, encodes a unique host-independent transcription system. This system comprises an eight-subunit RNA polymerase (vRNAP), temporally expressed transcription factors and transcript associated proteins, facilitating cross-species transmission via intermediate host. The protein composition of the virion and the presence of transcription factors in virus genome suggest existence of distinct transcription systems during viral infection. However, the precise mechanisms of transcription regulation remain elusive. Through analyses of dynamic transcriptome, vRNAP-associated components and cell-based assay, the critical role of M1249L in viral transcription regulation has been highlighted. Atomic-resolution structures of vRNAP-M1249L supercomplex, exhibiting a variety of conformations, have uncovered the dual functions of M1249L. During early transcription, M1249L could serve as multiple temporary transcription factors with C-terminal domain acting as a switcher for activation/inactivation, while during late transcription it aids in the packaging of the transcription machinery. The structural and functional characteristics of M1249L underscore its vital roles in ASFV transcription, packaging, and capsid assembly, presenting novel opportunities for therapeutic intervention.
リンク	Nat Commun / PubMed:39567541 / PubMed Central
手法	EM (単粒子)
解像度	2.56 - 9.32 Å
構造データ	39505 8yqt EMDB-39505, PDB-8yqt: African swine fever virus RNA Polymerase-M1249L complex2 手法: EM (単粒子) / 解像度: 2.56 Å 39506 8yqu EMDB-39506, PDB-8yqu: African swine fever virus RNA Polymerase-M1249L complex1 手法: EM (単粒子) / 解像度: 2.85 Å 39507 8yqv EMDB-39507, PDB-8yqv: African swine fever virus RNA Polymerase core 手法: EM (単粒子) / 解像度: 2.67 Å 39508 8yqw EMDB-39508, PDB-8yqw: ASFV RNA polymerase-M1249L complex3 手法: EM (単粒子) / 解像度: 2.68 Å 39509 8yqx EMDB-39509, PDB-8yqx: ASFV RNA polymerase-M1249L complex4 手法: EM (単粒子) / 解像度: 2.97 Å 39510 8yqy EMDB-39510, PDB-8yqy: ASFV RNA polymerase-M1249L complex complete 手法: EM (単粒子) / 解像度: 3.68 Å 39511 8yqz EMDB-39511, PDB-8yqz: African swine fever virus RNA Polymerase--DNA complex 手法: EM (単粒子) / 解像度: 2.78 Å EMDB-39512: ASFV RNA polymerase-M1249L complex 6 手法: EM (単粒子) / 解像度: 9.23 Å EMDB-39513: ASFV RNA polymerase-M1249L complex 7 手法: EM (単粒子) / 解像度: 5.17 Å EMDB-39514: ASFV RNA polymerase-M1249L complex 5 手法: EM (単粒子) / 解像度: 3.23 Å EMDB-39515: ASFV RNA polymerase-M1249L complex 9 手法: EM (単粒子) / 解像度: 4.04 Å EMDB-39516: ASFV RNA polymerase-M1249L complex 8 手法: EM (単粒子) / 解像度: 3.19 Å EMDB-39517: ASFV RNA polymerase-M1249L complex10 手法: EM (単粒子) / 解像度: 3.49 Å EMDB-39518: ASFV RNA polymerase-M1249L complex11 手法: EM (単粒子) / 解像度: 4.17 Å EMDB-39519: ASFV RNA polymerase-M1249L complex 12 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-39520: ASFV RNA polymerase-M1249L complex14 手法: EM (単粒子) / 解像度: 3.91 Å EMDB-39521: ASFV RNA polymerase-M1249L complex 13 手法: EM (単粒子) / 解像度: 9.32 Å EMDB-39522: ASFV RNA polymerase-M1249L complex 15 手法: EM (単粒子) / 解像度: 4.53 Å EMDB-39523: ASFV RNA polymerase-M1249L complex 16 手法: EM (単粒子) / 解像度: 3.59 Å EMDB-39524: ASFV RNA polymerase-M1249L complex 17 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-39525: ASFV RNA polymerase-M1249L complex 18 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-39526: ASFV RNA polymerase-M1249L complex 19 手法: EM (単粒子) / 解像度: 4.04 Å EMDB-39527: ASFV RNA polymerase-M1249L complex 20 手法: EM (単粒子) / 解像度: 3.86 Å EMDB-39528: ASFV RNA polymerase-M1249L complex 21 手法: EM (単粒子) / 解像度: 3.64 Å EMDB-39529: ASFV RNA polymerase-M1249L complex 24 手法: EM (単粒子) / 解像度: 4.04 Å EMDB-39530: ASFV RNA polymerase-M1249L complex 22 手法: EM (単粒子) / 解像度: 4.31 Å EMDB-39531: ASFV RNA polymerase-M1249L complex 23 手法: EM (単粒子) / 解像度: 6.99 Å EMDB-39536: ASFV RNA polymerase-M1249L complex 25 手法: EM (単粒子) / 解像度: 2.94 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry
由来	african swine fever virus (アフリカ豚コレラウイルス)
キーワード	VIRAL PROTEIN / ASFV / RNA polymerase / RNAP