Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural insights into the DNA topoisomerase II of the African swine fever virus.
Journal, issue, pages	Nat Commun, Vol. 15, Issue 1, Page 4607, Year 2024
Publish date	May 30, 2024
Authors	Jingyuan Cong / Yuhui Xin / Huiling Kang / Yunge Yang / Chenlong Wang / Dongming Zhao / Xuemei Li / Zihe Rao / Yutao Chen /
PubMed Abstract	Type II topoisomerases are ubiquitous enzymes that play a pivotal role in modulating the topological configuration of double-stranded DNA. These topoisomerases are required for DNA metabolism and ...Type II topoisomerases are ubiquitous enzymes that play a pivotal role in modulating the topological configuration of double-stranded DNA. These topoisomerases are required for DNA metabolism and have been extensively studied in both prokaryotic and eukaryotic organisms. However, our understanding of virus-encoded type II topoisomerases remains limited. One intriguing example is the African swine fever virus, which stands as the sole mammalian-infecting virus encoding a type II topoisomerase. In this work, we use several approaches including cryo-EM, X-ray crystallography, and biochemical assays to investigate the structure and function of the African swine fever virus type II topoisomerase, pP1192R. We determine the structures of pP1192R in different conformational states and confirm its enzymatic activity in vitro. Collectively, our results illustrate the basic mechanisms of viral type II topoisomerases, increasing our understanding of these enzymes and presenting a potential avenue for intervention strategies to mitigate the impact of the African swine fever virus.
External links	Nat Commun / PubMed:38816407 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	2.3 - 5.9 Å
Structure data	37225 8kgl EMDB-37225, PDB-8kgl: Structure of African swine fever virus topoisomerase II Method: EM (single particle) / Resolution: 4.3 Å 37226 8kgm EMDB-37226, PDB-8kgm: Structure of African swine fever virus topoisomerase II in complex with dsDNA Method: EM (single particle) / Resolution: 4.8 Å 37227 8kgn EMDB-37227, PDB-8kgn: Structure of African swine fever virus topoisomerase II in complex with dsDNA Method: EM (single particle) / Resolution: 5.9 Å 37228 8kgo EMDB-37228, PDB-8kgo: Structure of African swine fever virus topoisomerase II in complex with dsDNA Method: EM (single particle) / Resolution: 3.4 Å 37229 8kgp EMDB-37229, PDB-8kgp: Structure of African swine fever virus topoisomerase II in complex with dsDNA Method: EM (single particle) / Resolution: 3.3 Å 37230 8kgq EMDB-37230, PDB-8kgq: Structure of African swine fever virus topoisomerase II in complex with dsDNA Method: EM (single particle) / Resolution: 5.6 Å 37231 8kgr EMDB-37231, PDB-8kgr: Structure of African swine fever virus topoisomerase II in complex with dsDNA Method: EM (single particle) / Resolution: 3.2 Å PDB-8kgs: Structure of African swine fever virus topoisomerase II in complex with dsDNA Method: X-RAY DIFFRACTION / Resolution: 2.603 Å PDB-8kgt: Structure of African swine fever virus topoisomerase II in complex with dsDNA Method: X-RAY DIFFRACTION / Resolution: 2.3 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogueYM ChemComp-HOH: WATER ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM
Source	african swine fever virus african swine fever virus lis57 dna molecule (others)
Keywords	VIRAL PROTEIN / topo 2 / VIRAL PROTEIN/DNA / VIRAL PROTEIN-DNA COMPLEX