Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of amine odorant perception by a mammal olfactory receptor.
Journal, issue, pages	Nature, Vol. 618, Issue 7963, Page 193-200, Year 2023
Publish date	May 24, 2023
Authors	Lulu Guo / Jie Cheng / Shuo Lian / Qun Liu / Yan Lu / Yuan Zheng / Kongkai Zhu / Minghui Zhang / Yalei Kong / Chao Zhang / Naikang Rong / Yuming Zhuang / Guoxing Fang / Jingjing Jiang / Tianyao Zhang / Xiang Han / Zili Liu / Ming Xia / Shangming Liu / Lei Zhang / Stephen D Liberles / Xiao Yu / Yunfei Xu / Fan Yang / Qian Li / Jin-Peng Sun /
PubMed Abstract	Odorants are detected as smell in the nasal epithelium of mammals by two G-protein-coupled receptor families, the odorant receptors and the trace amine-associated receptors (TAARs). TAARs emerged ...Odorants are detected as smell in the nasal epithelium of mammals by two G-protein-coupled receptor families, the odorant receptors and the trace amine-associated receptors (TAARs). TAARs emerged following the divergence of jawed and jawless fish, and comprise a large monophyletic family of receptors that recognize volatile amine odorants to elicit both intraspecific and interspecific innate behaviours such as attraction and aversion. Here we report cryo-electron microscopy structures of mouse TAAR9 (mTAAR9) and mTAAR9-G or mTAAR9-G trimers in complex with β-phenylethylamine, N,N-dimethylcyclohexylamine or spermidine. The mTAAR9 structures contain a deep and tight ligand-binding pocket decorated with a conserved DWY motif, which is essential for amine odorant recognition. In the mTAAR9 structure, a unique disulfide bond connecting the N terminus to ECL2 is required for agonist-induced receptor activation. We identify key structural motifs of TAAR family members for detecting monoamines and polyamines and the shared sequence of different TAAR members that are responsible for recognition of the same odour chemical. We elucidate the molecular basis of mTAAR9 coupling to G and G by structural characterization and mutational analysis. Collectively, our results provide a structural basis for odorant detection, receptor activation and G coupling of an amine olfactory receptor.
External links	Nature / PubMed:37225986
Methods	EM (single particle)
Resolution	2.97 - 3.49 Å
Structure data	35705 8itf EMDB-35705, PDB-8itf: Cryo-EM structure of the DMCHA-bound mTAAR9-Gs complex Method: EM (single particle) / Resolution: 3.46 Å 35761 8iw1 EMDB-35761, PDB-8iw1: Cryo-EM structure of the PEA-bound mTAAR9-Golf complex Method: EM (single particle) / Resolution: 3.4 Å 35762 8iw4 EMDB-35762, PDB-8iw4: Cryo-EM structure of the SPE-bound mTAAR9-Gs complex Method: EM (single particle) / Resolution: 3.49 Å 35763 8iw7 EMDB-35763, PDB-8iw7: Cryo-EM structure of the PEA-bound mTAAR9-Gs complex Method: EM (single particle) / Resolution: 2.97 Å 35764 8iw9 EMDB-35764, PDB-8iw9: Cryo-EM structure of the CAD-bound mTAAR9-Gs complex Method: EM (single particle) / Resolution: 3.08 Å 35765 8iwe EMDB-35765, PDB-8iwe: Cryo-EM structure of the SPE-mTAAR9 complex Method: EM (single particle) / Resolution: 3.4 Å 35771 8iwm EMDB-35771, PDB-8iwm: Cryo-EM structure of the PEA-bound mTAAR9 complex Method: EM (single particle) / Resolution: 3.17 Å
Chemicals	ChemComp-8IA: ~{N},~{N}-dimethylcyclohexanamine ChemComp-SPD: SPERMIDINE / Spermidine ChemComp-PEA: 2-PHENYLETHYLAMINE / alkaloid*YM / Phenethylamine
Source	mus musculus (house mouse) homo sapiens (human) synthetic construct (others) escherichia coli bl21(de3) (bacteria)
Keywords	MEMBRANE PROTEIN / DMCHA / mTAAR9 / PEA / SPE / CAD