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Open data
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Basic information
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Title | Cryo-EM structure of the SPE-mTAAR9 complex | |||||||||
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![]() | SPE / mTAAR9 / ![]() | |||||||||
Function / homology | ![]() Amine ligand-binding receptors / trace-amine receptor activity / G alpha (s) signalling events / ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Sun JP / Li Q / Yang F / Xu YF / Guo LL / Lian S / Zhang MH / Rong NK | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of amine odorant perception by a mammal olfactory receptor. Authors: Lulu Guo / Jie Cheng / Shuo Lian / Qun Liu / Yan Lu / Yuan Zheng / Kongkai Zhu / Minghui Zhang / Yalei Kong / Chao Zhang / Naikang Rong / Yuming Zhuang / Guoxing Fang / Jingjing Jiang / ...Authors: Lulu Guo / Jie Cheng / Shuo Lian / Qun Liu / Yan Lu / Yuan Zheng / Kongkai Zhu / Minghui Zhang / Yalei Kong / Chao Zhang / Naikang Rong / Yuming Zhuang / Guoxing Fang / Jingjing Jiang / Tianyao Zhang / Xiang Han / Zili Liu / Ming Xia / Shangming Liu / Lei Zhang / Stephen D Liberles / Xiao Yu / Yunfei Xu / Fan Yang / Qian Li / Jin-Peng Sun / ![]() ![]() Abstract: Odorants are detected as smell in the nasal epithelium of mammals by two G-protein-coupled receptor families, the odorant receptors and the trace amine-associated receptors (TAARs). TAARs emerged ...Odorants are detected as smell in the nasal epithelium of mammals by two G-protein-coupled receptor families, the odorant receptors and the trace amine-associated receptors (TAARs). TAARs emerged following the divergence of jawed and jawless fish, and comprise a large monophyletic family of receptors that recognize volatile amine odorants to elicit both intraspecific and interspecific innate behaviours such as attraction and aversion. Here we report cryo-electron microscopy structures of mouse TAAR9 (mTAAR9) and mTAAR9-G or mTAAR9-G trimers in complex with β-phenylethylamine, N,N-dimethylcyclohexylamine or spermidine. The mTAAR9 structures contain a deep and tight ligand-binding pocket decorated with a conserved DWY motif, which is essential for amine odorant recognition. In the mTAAR9 structure, a unique disulfide bond connecting the N terminus to ECL2 is required for agonist-induced receptor activation. We identify key structural motifs of TAAR family members for detecting monoamines and polyamines and the shared sequence of different TAAR members that are responsible for recognition of the same odour chemical. We elucidate the molecular basis of mTAAR9 coupling to G and G by structural characterization and mutational analysis. Collectively, our results provide a structural basis for odorant detection, receptor activation and G coupling of an amine olfactory receptor. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 16.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.1 KB 15.1 KB | Display Display | ![]() |
Images | ![]() | 67.7 KB | ||
Others | ![]() ![]() | 17 MB 17 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8iweMC ![]() 8itfC ![]() 8iw1C ![]() 8iw4C ![]() 8iw7C ![]() 8iw9C ![]() 8iwmC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.893 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_35765_half_map_2.map | ||||||||||||
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Density Histograms |
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Sample components
-Entire : Cryo-EM structure of the SPE-bound mTAAR9 complex
Entire | Name: Cryo-EM structure of the SPE-bound mTAAR9 complex |
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Components |
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-Supramolecule #1: Cryo-EM structure of the SPE-bound mTAAR9 complex
Supramolecule | Name: Cryo-EM structure of the SPE-bound mTAAR9 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Macromolecule #1: Trace amine-associated receptor 9
Macromolecule | Name: Trace amine-associated receptor 9 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 38.928238 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MTSDFSPEPP MELCYENVNG SCIKSSYAPW PRAILYGVLG LGALLAVFGN LLVIIAILHF KQLHTPTNFL VASLACADFL VGVTVMPFS TVRSVESCWY FGESYCKFHT CFDTSFCFAS LFHLCCISID RYIAVTDPLT YPTKFTVSVS GLCIALSWFF S VTYSFSIF ...String: MTSDFSPEPP MELCYENVNG SCIKSSYAPW PRAILYGVLG LGALLAVFGN LLVIIAILHF KQLHTPTNFL VASLACADFL VGVTVMPFS TVRSVESCWY FGESYCKFHT CFDTSFCFAS LFHLCCISID RYIAVTDPLT YPTKFTVSVS GLCIALSWFF S VTYSFSIF YTGANEEGIE ELVVALTCVG GCQAPLNQNW VLLCFLLFFL PTVVMVFLYG RIFLVAKYQA RKIEGTANQA QA SSESYKE RVAKRERKAA KTLGIAMAAF LVSWLPYIID AVIDAYMNFI TPAYVYEILV WCVYYNSAMN PLIYAFFYPW FRK AIKLIV SGKVFRADSS TTNLFSEEAG AG |
-Macromolecule #2: SPERMIDINE
Macromolecule | Name: SPERMIDINE / type: ligand / ID: 2 / Number of copies: 1 / Formula: SPD |
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Molecular weight | Theoretical: 145.246 Da |
Chemical component information | ![]() ChemComp-SPD: |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.875 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: OTHER |
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Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 193217 |