EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 10, Page 1532-1542, Year 2024
掲載日	2024年6月18日
著者	Qian Li / Jun Zhang / Cory Haluska / Xiang Zhang / Lei Wang / Guangfeng Liu / Zhaoning Wang / Duo Jin / Tong Cheng / Hongxia Wang / Yuan Tian / Xiangxi Wang / Lei Sun / Xiaolan Zhao / Zhenguo Chen / Lanfeng Wang /
PubMed 要旨	Smc5/6 is a member of the eukaryotic structural maintenance of chromosomes (SMC) family of complexes with important roles in genome maintenance and viral restriction. However, limited structural ...Smc5/6 is a member of the eukaryotic structural maintenance of chromosomes (SMC) family of complexes with important roles in genome maintenance and viral restriction. However, limited structural understanding of Smc5/6 hinders the elucidation of its diverse functions. Here, we report cryo-EM structures of the budding yeast Smc5/6 complex in eight-subunit, six-subunit and five-subunit states. Structural maps throughout the entire length of these complexes reveal modularity and key elements in complex assembly. We show that the non-SMC element (Nse)2 subunit supports the overall shape of the complex and uses a wedge motif to aid the stability and function of the complex. The Nse6 subunit features a flexible hook region for attachment to the Smc5 and Smc6 arm regions, contributing to the DNA repair roles of the complex. Our results also suggest a structural basis for the opposite effects of the Nse1-3-4 and Nse5-6 subcomplexes in regulating Smc5/6 ATPase activity. Collectively, our integrated structural and functional data provide a framework for understanding Smc5/6 assembly and function.
リンク	Nat Struct Mol Biol / PubMed:38890552 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 8.5 Å
構造データ	33914 7ylm EMDB-33914, PDB-7ylm: Cryo-EM structure of 8-subunit Smc5/6 hinge region 手法: EM (単粒子) / 解像度: 6.17 Å 33927 7ymd EMDB-33927, PDB-7ymd: Cryo-EM structure of Nse1/3/4 手法: EM (単粒子) / 解像度: 4.176 Å 34025 7yqh EMDB-34025, PDB-7yqh: Cryo-EM structure of 8-subunit Smc5/6 手法: EM (単粒子) / 解像度: 5.6 Å 34953 8hqs EMDB-34953, PDB-8hqs: Cryo-EM structure of 8-subunit Smc5/6 head region 手法: EM (単粒子) / 解像度: 3.2 Å 35116 8i13 EMDB-35116, PDB-8i13: Cryo-EM structure of 6-subunit Smc5/6 手法: EM (単粒子) / 解像度: 6.9 Å 35128 8i21 EMDB-35128, PDB-8i21: Cryo-EM structure of 6-subunit Smc5/6 arm region 手法: EM (単粒子) / 解像度: 6.02 Å 35184 8i4u EMDB-35184, PDB-8i4u: Cryo-EM structure of 5-subunit Smc5/6 hinge region 手法: EM (単粒子) / 解像度: 6.73 Å 35185 8i4v EMDB-35185, PDB-8i4v: Cryo-EM structure of 5-subunit Smc5/6 arm region 手法: EM (単粒子) / 解像度: 5.97 Å 35186 8i4w EMDB-35186, PDB-8i4w: Cryo-EM structure of 5-subunit Smc5/6 head region 手法: EM (単粒子) / 解像度: 6.01 Å 35187 8i4x EMDB-35187, PDB-8i4x: Cryo-EM structure of 5-subunit Smc5/6 手法: EM (単粒子) / 解像度: 8.5 Å 37584 8wjl EMDB-37584, PDB-8wjl: Cryo-EM structure of 6-subunit Smc5/6 hinge region 手法: EM (単粒子) / 解像度: 6.15 Å 37586 8wjn EMDB-37586, PDB-8wjn: Cryo-EM structure of 6-subunit Smc5/6 head region 手法: EM (単粒子) / 解像度: 5.58 Å 37587 8wjo EMDB-37587, PDB-8wjo: Cryo-EM structure of 8-subunit Smc5/6 arm region 手法: EM (単粒子) / 解像度: 6.04 Å EMDB-60245: Smc5/6-5mer consensus map 手法: EM (単粒子) / 解像度: 8.5 Å EMDB-60246: Smc5/6-6mer consensus map 手法: EM (単粒子) / 解像度: 6.9 Å
由来	saccharomyces cerevisiae (パン酵母) saccharomyces cerevisiae s288c (パン酵母)
キーワード	CELL CYCLE