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TitleStructural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT.
Journal, issue, pagesScience, Vol. 377, Issue 6611, Page eabp9466, Year 2022
Publish dateSep 9, 2022
AuthorsHaruhiko Ehara / Tomoya Kujirai / Mikako Shirouzu / Hitoshi Kurumizaka / Shun-Ichi Sekine /
PubMed AbstractDuring gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII ...During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII elongation complex (EC) passing through a nucleosome in the presence of the transcription elongation factors Spt6, Spn1, Elf1, Spt4/5, and Paf1C and the histone chaperone FACT (facilitates chromatin transcription). The structures show snapshots of EC progression on DNA mediating downstream nucleosome disassembly, followed by its reassembly upstream of the EC, which is facilitated by FACT. FACT dynamically adapts to successively occurring subnucleosome intermediates, forming an interface with the EC. Spt6, Spt4/5, and Paf1C form a "cradle" at the EC DNA-exit site and support the upstream nucleosome reassembly. These structures explain the mechanism by which the EC traverses nucleosomes while maintaining the chromatin structure and epigenetic information.
External linksScience / PubMed:35981082
MethodsEM (single particle)
Resolution3.1 - 4.2 Å
Structure data

33313

7xn7

EMDB-33313, PDB-7xn7:
RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt6, Spn1 and Paf1C
Method: EM (single particle) / Resolution: 3.1 Å

33424

7xse

EMDB-33424, PDB-7xse:
RNA polymerase II elongation complex transcribing a nucleosome (EC42)
Method: EM (single particle) / Resolution: 3.6 Å

33436

7xsx

EMDB-33436, PDB-7xsx:
RNA polymerase II elongation complex transcribing a nucleosome (EC49)
Method: EM (single particle) / Resolution: 3.8 Å

33437

7xsz

EMDB-33437, PDB-7xsz:
RNA polymerase II elongation complex transcribing a nucleosome (EC115)
Method: EM (single particle) / Resolution: 3.4 Å

33441

7xt7

EMDB-33441, PDB-7xt7:
RNA polymerase II elongation complex transcribing a nucleosome (EC49B)
Method: EM (single particle) / Resolution: 4.2 Å

33447

7xtd

EMDB-33447, PDB-7xtd:
RNA polymerase II elongation complex transcribing a nucleosome (EC58oct)
Method: EM (single particle) / Resolution: 3.9 Å

33450

7xti

EMDB-33450, PDB-7xti:
RNA polymerase II elongation complex transcribing a nucleosome (EC58hex)
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • komagataella phaffii (fungus)
  • synthetic construct (others)
  • homo sapiens (human)
KeywordsTRANSCRIPTION / chromatin / nucleosome / TRANSCRIPTION/DNA/RNA / TRANSCRIPTION-DNA-RNA complex

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