EMDB-33313: RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt...

Basic information

Entry

Database: EMDB / ID: EMD-33313

• Title: RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt6, Spn1 and Paf1C

Sample

• Complex: RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt6, Spn1 and Paf1C
  • Protein or peptide: x 22 types
  • DNA: x 2 types
  • RNA: x 1 types
• Ligand: x 2 types

• Keywords: chromatin / nucleosome / TRANSCRIPTION

Function / homology

Function:

RNA polymerase II C-terminal domain phosphoserine binding / regulation of septum digestion after cytokinesis / Cdc73/Paf1 complex / siRNA-mediated pericentric heterochromatin formation / DSIF complex / chromatin-protein adaptor activity / RPB4-RPB7 complex / nucleosome organization / transcription elongation factor activity / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / poly(A)+ mRNA export from nucleus / termination of RNA polymerase II transcription / transcription elongation-coupled chromatin remodeling / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / termination of RNA polymerase III transcription / termination of RNA polymerase I transcription / transcription initiation at RNA polymerase III promoter / RNA polymerase II complex binding / transcription initiation at RNA polymerase I promoter / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / positive regulation of translational initiation / chromosome, centromeric region / translation elongation factor activity / RNA polymerase I complex / tRNA transcription by RNA polymerase III / RNA polymerase III complex / transcription elongation by RNA polymerase I / pericentric heterochromatin / transcription-coupled nucleotide-excision repair / RNA polymerase II, core complex / : / nucleosome binding / translation initiation factor binding / DNA-directed RNA polymerase activity / transcription elongation factor complex / regulation of DNA-templated transcription elongation / positive regulation of transcription elongation by RNA polymerase II / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / P-body / ribonucleoside binding / : / : / : / : / : / : / DNA-directed RNA polymerase / single-stranded DNA binding / histone binding / nucleic acid binding / single-stranded RNA binding / protein dimerization activity / RNA-directed RNA polymerase activity / nucleotide binding / mRNA binding / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / nucleolus / DNA binding / RNA binding / zinc ion binding / nucleus / metal ion binding

Domain/homology:

: / Spt6, S1/OB domain / : / Leo1-like protein / Leo1-like protein / Plus-3 domain / Plus3-like superfamily / Plus-3 domain / Plus3 domain profile. / Short conserved domain in transcriptional regulators. / RNA polymerase II associated factor Paf1 / Paf1 / Cdc73/Parafibromin / RNA polymerase-associated protein Ctr9 / Cell division control protein 73, C-terminal / Cell division control protein 73, C-terminal domain superfamily / RNA pol II accessory factor, Cdc73 family, C-terminal / Transcription elongation factor 1 / Transcription elongation factor 1 superfamily / Transcription elongation factor Elf1 like / Spt5 C-terminal nonapeptide repeat binding Spt4 / Spt6 acidic, N-terminal domain / Helix-turn-helix DNA-binding domain of Spt6 / Transcription elongation factor Spt6, YqgF domain / Transcription elongation factor Spt6, helix-hairpin-helix motif / Spt6, SH2 domain, C terminus / Acidic N-terminal SPT6 / Helix-hairpin-helix motif / Holliday-junction resolvase-like of SPT6 / Helix-turn-helix DNA-binding domain of SPT6 / Tex-like protein, HTH domain superfamily / Tex-like domain superfamily / Spt6, Death-like domain / : / Tex central region-like / Transcription elongation factor Spt6 / Spt6, SH2 domain, N terminus / Spt6, SH2 domain / SH2 domain / YqgF/RNase H-like domain superfamily / Transcription initiation Spt4 / Spt4 superfamily / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / TFIIS N-terminal domain profile. / Transcription factor IIS, N-terminal / TFIIS helical bundle-like domain / Transcription elongation factor Spt5, eukaryote / Spt5 transcription elongation factor, N-terminal / Spt5, KOW domain repeat 2 / Spt5, KOW domain repeat 3 / Spt5, KOW domain repeat 5 / Spt5 transcription elongation factor, acidic N-terminal / NGN domain, eukaryotic / Spt5, KOW domain repeat 1 / Spt5, KOW domain repeat 4 / NGN domain / Transcription elongation factor SPT5 / Early transcription elongation factor of RNA pol II, NGN section / RuvA domain 2-like / TFIIS/LEDGF domain superfamily / Tetratricopeptide repeat / NusG, N-terminal domain superfamily / DNA-directed RNA polymerase II subunit Rpb4-like / RNA polymerase Rpb1 C-terminal repeat / RNA polymerase II, heptapeptide repeat, eukaryotic / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Pol II subunit B9, C-terminal zinc ribbon / RNA polymerase RBP11 / Rpb4/RPC9 superfamily / S1 domain profile. / RNA polymerase subunit Rpb4/RPC9 / RNA polymerase Rpb4 / HRDC-like superfamily / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / RNA polymerase subunit Rpb7-like / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / : / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8

Component:

Transcription elongation factor 1 homolog / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Transcription elongation factor SPT4 / Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p / RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / RNA polymerase II subunit B32 / Transcription elongation factor SPT5 / RNA polymerase-associated protein LEO1 / RNA polymerase II subunit B12.5 / DNA-directed RNA polymerase subunit / Component of the Paf1p complex / Transcription elongation factor Spt6 / RNA polymerase II third largest subunit B44, part of central core / Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II / RNAP II-associated protein / DNA-directed RNA polymerase subunit / RNA polymerase subunit ABC10-alpha / DNA-directed RNA polymerase subunit / RNAPII-associated chromatin remodeling Paf1 complex subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC1

• Biological species: Komagataella phaffii (fungus) / synthetic construct (others)
• Method: single particle reconstruction / cryo EM / Resolution: 3.1 Å
• Authors: Ehara H / Kujirai T / Shirouzu M / Kurumizaka H / Sekine S

Funding support

Japan, 7 items

Organization	Grant number	Country
Japan Society for the Promotion of Science (JSPS)	JP20H03201	Japan
Japan Society for the Promotion of Science (JSPS)	JP20H05690	Japan
Japan Society for the Promotion of Science (JSPS)	JP20H00449	Japan
Japan Society for the Promotion of Science (JSPS)	JP18H05534	Japan
Japan Agency for Medical Research and Development (AMED)	JP20am0101082	Japan
Japan Agency for Medical Research and Development (AMED)	JP21am0101076	Japan
Japan Science and Technology	JPMJER1901	Japan

• Citation: Journal: Science / Year: 2022; Title: Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT.; Authors: Haruhiko Ehara / Tomoya Kujirai / Mikako Shirouzu / Hitoshi Kurumizaka / Shun-Ichi Sekine / ; Abstract: During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII elongation complex (EC) passing through a nucleosome in the presence of the transcription elongation factors Spt6, Spn1, Elf1, Spt4/5, and Paf1C and the histone chaperone FACT (facilitates chromatin transcription). The structures show snapshots of EC progression on DNA mediating downstream nucleosome disassembly, followed by its reassembly upstream of the EC, which is facilitated by FACT. FACT dynamically adapts to successively occurring subnucleosome intermediates, forming an interface with the EC. Spt6, Spt4/5, and Paf1C form a "cradle" at the EC DNA-exit site and support the upstream nucleosome reassembly. These structures explain the mechanism by which the EC traverses nucleosomes while maintaining the chromatin structure and epigenetic information.

History

Deposition	Apr 28, 2022	-
Header (metadata) release	Sep 7, 2022	-
Map release	Sep 7, 2022	-
Update	Jul 3, 2024	-
Current status	Jul 3, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_33313.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.484 Å

Density

Contour Level	By AUTHOR: 0.015
Minimum - Maximum	-0.042013798 - 0.1266646
Average (Standard dev.)	0.0005715849 (±0.0046577416)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 240 240 240 Spacing 240 240 240
Cell	A=B=C: 356.16 Å α=β=γ: 90.0 °

Supplemental data

Additional map: Map3

• File: emd_33313_additional_1.map
• Annotation: Map3

Additional map: Map2

• File: emd_33313_additional_2.map
• Annotation: Map2

Additional map: Map1

• File: emd_33313_additional_3.map
• Annotation: Map1

Additional map: Map4

• File: emd_33313_additional_4.map
• Annotation: Map4

Additional map: Map5

• File: emd_33313_additional_5.map
• Annotation: Map5

Additional map: Map6

• File: emd_33313_additional_6.map
• Annotation: Map6

Additional map: Map7

• File: emd_33313_additional_7.map
• Annotation: Map7

Half map: #2

• File: emd_33313_half_map_1.map

Half map: #1

• File: emd_33313_half_map_2.map

Sample components

Entire : RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt...

• Entire: Name: RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt6, Spn1 and Paf1C

Components

• Complex: RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt6, Spn1 and Paf1C
  • Protein or peptide: DNA-directed RNA polymerase subunit
  • Protein or peptide: DNA-directed RNA polymerase subunit beta
  • Protein or peptide: RNA polymerase II third largest subunit B44, part of central core
  • Protein or peptide: RNA polymerase II subunit B32
  • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC1
  • Protein or peptide: RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III
  • Protein or peptide: RNA polymerase II subunit
  • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
  • Protein or peptide: DNA-directed RNA polymerase subunit
  • Protein or peptide: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
  • Protein or peptide: RNA polymerase II subunit B12.5
  • Protein or peptide: RNA polymerase subunit ABC10-alpha
  • Protein or peptide: Transcription elongation factor 1 homolog
  • DNA: DNA (198-mer)
  • RNA: RNA (5'-R(P*AP*AP*GP*CP*CP*UP*GP*GP*UP*GP*UP*CP*UP*UP*GP*GP*GP*UP*G)-3')
  • DNA: DNA (198-mer)
  • Protein or peptide: Transcription elongation factor SPT4
  • Protein or peptide: Chromatin elongation factor SPT5
  • Protein or peptide: Transcription elongation factor Spt6
  • Protein or peptide: Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II
  • Protein or peptide: Component of the Paf1p complex
  • Protein or peptide: RNAPII-associated chromatin remodeling Paf1 complex subunit
  • Protein or peptide: Leo1
  • Protein or peptide: RNAP II-associated protein
  • Protein or peptide: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION

Supramolecule #1: RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt...

• Supramolecule: Name: RNA polymerase II elongation complex containing Spt4/5, Elf1, Spt6, Spn1 and Paf1C; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#25
• Source (natural): Organism: Komagataella phaffii (fungus)

Macromolecule #1: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Komagataella phaffii (fungus)
• Molecular weight: Theoretical: 194.107422 KDa

Sequence

String:

MSQFPYSSAP LRSVKEVQFG LLSPEEIRAI SVVKIEYPEI MDESRQRPRE GGLNDPKLGS IDRNFKCQTC GEGMAECPGH FGHMELAKP VFHIGFIPKI KKVCECICMN CGKLLLDETN PTMAQAIRIR DPKKRFNAVW QLCKTKMVCE ADAPVDEYSE Q KVVSRGGC GNTQPVVRKD GMKLWGTWKK SGFSDRDAQP ERKLLTPGEI LNVFKHISPE DCFRLGFNED YARPEWMIIT VL PVPPPQV RPSIAMDETT QGQDDLTHKL SDILKANINV QKLEMDGSPQ HIINEVEQLL QFHVATYMDN DIAGQPQALQ KSG RPVKAI RARLKGKEGR LRGNLMGKRV DFSARTVISG DPNLELDQVG VPISIAKTLS YPETVTQYNI HRLTEYVRNG PNEH PGAKY VIRDNGDRID LRYHKRAGDI VLQYGWKVER HLMDDDPVLF NRQPSLHKMS MMAHRVKVMP YSTFRLNLSV TSPYN ADFD GDEMNLHVPQ SEETRAELSQ LCAVPLQIVS PQSNKPVMGI VQDTLCGVRK MTLRDTFIEY EQVMNMLFWV PSWDGV VPQ PAILKPKPLW TGKQLLSIAI PSGIHLQRTD GGNSLLSPKD NGMLIVDGKV MFGVVDKKTV GSGGGGLIHT VMREKGP KI CAELFGNIQK VVNYWLLHNG FSIGIGDAIA DASTMKEITH AISSAKEQVQ EIIYKAQHNE LELKPGMTLR ESFEGEVS R TLNDARDSAG RSAEMNLKDL NNVKQMVSAG SKGSFINIAQ MSACVGQQMV EGKRIAFGFA DRSLPHFTKD DFSPESKGF VENSYLRGLT PQEFFFHAMA GREGLIDTAV KTAETGYIQR RLVKALEDIM VHYDGTTRNS LGDIIQFLYG EDGLDGTQVE RQTIDTIPG SDKAFHKRYY VDLMDEKNSI KPDVIEYAAD ILGDVELQKE LNSEYEQLVS DRKFLREIVF VNGDHNWPLP V NLRRIIQN AQQIFHLDRA KASDLTIPEI IHGVRDLCKK LFVLRGENEL IKEAQQNATS LFQCLVRARL ATRRILEEFR LN RDAFEWV LGTIEAQFQR SLVHPGEMVG VIAAQSIGEP ATQMTLNTFH YAGVSSKNVT LGVPRLKEIL NVAKNIKTPA LTV YLDREI ALDIEKAKVI QSSIEYTTLK NVTSATEIYY DPDPTSTVIE EDFDTVEAYF SIPDEKVEET IDKQSPWLLR LELD RARML DKQLTMNQVA DKISEVFSDD LFVMWSEDNA DKLIIRCRVI RDPKAMDEEL EAEEDQMLKR IEAHMLDLIA LRGIP GISK VYMVKHKVSV PDESGEYKNE ELWALETDGI NLAEVMAVPG VDSSRTYSNS FVEILSVLGI EATRSSLYKE ILNVIA FDG SYVNYRHMAL LVDVMTSRGY LMAITRHGIN RADTGALMRC SFEETVEILF EAGAAAELDD CRGVSENVML GQLAPMG TG AFDVMIDEKL LTSLPADYAP TMPLFKGKAT QGSATPYDNN AQYDDEFNHD DVADVMFSPM AETGSGDDRS GGLTEYAG I QSPYQPTSPG LSATSPGFAP TSPGFAPTSP RYSPTSPGYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPQY SPTSPQYSPT SPQYSPTSPQ YSPTSPQYSP TSPQYSPTSP QYSPTSPQYS PTSPQYSPT SPQYSPTSPQ YSPTSPQYSP TSPQYSPTSP QYSPASPQYS PSRHSPNGES KEGE

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #2: DNA-directed RNA polymerase subunit beta

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 139.746094 KDa

Sequence

String:

MSYDPYSIDD TITTEDCWTV ISAFFEEKGL VSQQLDSFDE FMETSIQDLV WEEPRLILDQ PAQHTNEKDN INKRYEIRFG KIYLSRPTM TEADGTTHAM FPQEARLRNL TYSSPVYLDM EKSMFTSIDD EGNPNATLDW QQVHEPIKDG VEEGNKVHIG K VPIMLRSK FCSLRTLDEV DLYKMKECPY DMGGYFVING SEKVLIAQER SAANIVQVFK KAAPSPISHV AEIRSALEKG SR LISTMQI KLYGREDKGT GRTIKATLPY VKQDIPIVIV FRALGVVPDG EILQHICYDE NDWQMLEMLK PCIEEGFVIQ DKE VALDFI GRRGSAALGI RREKRIQYAK DILQKELLPH ITQEEGFETR KTFFLGYMVN RLLLCALERK DQDDRDHFGK KRLD LAGPL LANLFRILFR KLTREIYRYM QRCIETDRDF NLNLAVKSTT ITSGLKYSLA TGNWGEQKKA MSSRAGVSQV LNRYT YSST LSHLRRTNTP IGRDGKLAKP RQLHNTHWGL VCPAETPEGQ ACGLVKNLSL LSGISIGSPS EPIINFLEEW GMEPLE DYD PAQHTKSTRI FVNGVWTGIH RDPSMLVSTM RDLRRSGAIS PEVSIIRDIR EREFKIFTDV GRVYRPLFIV EDDESKD NK GELRITKEHI RKIQQGYDDD AMNDDSEEQE QDVYGWSSLV TSGVIEYVDG EEEETIMIAM TPEDLQTRSL EQKEIDLN D TAKRIKPEMS TSSHHTFTHC EIHPSMILGV AASIIPFPDH NQSPRNTYQS AMGKQAMGVF LTNYNVRMDT MANILYYPQ KPLAKTQAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL FRSLFFRSYM DQEKRFGISI VEEFEKPTRA TTLRLKHGT YEKLDEDGLI APGVRVSGDD IIIGKTTPIP PDTEELGQRT KYHTKRDAST PLRSTENGIV DQVLLTTNQE G LKFVKVRM RTTKVPQIGD KFASRHGQKG TIGVTYRHED MPFSAEGIVP DLIINPHAIP SRMTVAHLIE CLLSKVGSIR GY EGDATPF TDLTVDAVSN LLRDNGYQSR GFEVMYNGHT GKKLMAQVFF GPTYYQRLRH MVDDKIHARA RGPVQVLTRQ PVE GRSRDG GLRFGEMERD CMIAHGAAGF LKERLMEASD AFRVHVCGIC GLMSVIANLK KNQFECRSCK NKTNIYQLHI PYAA KLLFQ ELMAMNIAPR LYTERSGVSM RS

UniProtKB: DNA-directed RNA polymerase subunit beta

Macromolecule #3: RNA polymerase II third largest subunit B44, part of central core

• Macromolecule: Name: RNA polymerase II third largest subunit B44, part of central core; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 34.216293 KDa

Sequence

String:

MSKEPKVNII NAQDDEVELM LSDVNLSLAN SLRRTMLAEV PTLAIDLVEI KMNTSVLADE FISHRLGLIP LVSEDVEEMK YSRDCTCED YCDECSVVLE LSARHEGEEG TTDVYSSSLI KVSGPGNLNV GEPVRRDDYD QGILLCKLRN HQELNIRCIA K KGIAKEHA KWSPCSAIAF EYDPHNKLKH TDFWFEVDAK KEWPDSKYAT WEEPPKPGEV FDYKAKPNRF YMTVETTGSL KA NQVFSRG IKTLQEKLAN VLFELENSRP ANTTAYGGAT AYGGQTVYGR ETSYGGNTNY GDYNAPY

UniProtKB: RNA polymerase II third largest subunit B44, part of central core

Macromolecule #4: RNA polymerase II subunit B32

• Macromolecule: Name: RNA polymerase II subunit B32 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 20.62298 KDa

Sequence

String:

MNVSTSTVGA RRRRAKQQVD DEENATLLRL GPEFALKQYD HDGNEHDLIA LSLSESRLLI REALKARSRA RNGGVDIESS NGEIDDDEL AKVTSGAVAN GVVKKTLDYL NTFARFKDEE TCTAVDQLLH NSSDCSVLHP FEIAQLSSLG CEDVDEAITL I PSLAAKKE VNLQRILDEL NRLEDPYK

UniProtKB: RNA polymerase II subunit B32

Macromolecule #5: DNA-directed RNA polymerases I, II, and III subunit RPABC1

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1; type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus)
• Molecular weight: Theoretical: 24.96268 KDa

Sequence

String:

MEDNNRIISR LWRSFRTVKE MAADRGYFIS QEEMDQSLEE FRSKICDSMG NPQRKLMSFL ANPTPEALEK YSDLGTLWVE FCDEPSVGI KTMRNFCLRI QEKNFSTGIF IYQNNITPSA NKMIPTVSPA IIETFQESDL VVNITHHELV PKHIRLSDGE K SQLLQRYK LKESQLPRIQ REDPVARYLG LKRGQVVKII RRSETSGRYA SYRICL

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

Macromolecule #6: RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III

• Macromolecule: Name: RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 17.803588 KDa

Sequence

String:

MSEDEAFNEQ TENFENFEDE HFSDDNFEDR STQPEDYAVG VTADGRQIIN GDGIQEVNGT IKAHRKRSNK ELAILKEERT TTPYLTKYE RARILGTRAL QISMNAPVLV DIEGETDPLQ IAMKELSQRK IPLVIRRYLP DGSYEDWGCD ELIVDN

UniProtKB: RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III

Macromolecule #7: RNA polymerase II subunit

• Macromolecule: Name: RNA polymerase II subunit / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 18.802625 KDa

Sequence

String:

MFFLKDLSLI LTLHPSYFGP QMNQYLREKL LTDVEGTCTG QFGYIVTVLD GMNIDVGKGR IIPGSGSAEF EVKYRAVVWK PFKGEVVDA IVSNVSPIGF FADVGPLNVF VSTRLIPDNL VYNPSNSPPA YMSNDELITK GSKVRLKVVG TRTDVNEIYA I GSIKEDFL GAI

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #8: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 16.24922 KDa

Sequence

String:

MSSALFDDIF TVQTVDNGRY NKVSRIIGIS TTNSAIKLTL DINNEMFPVS QDDSLTVTLA NSLSLDGEDE SANFSKSWRP PKPTDKSLA DDYDYVMFGT VYKFEEGDED KIKVYVSFGG LLMCLEGGYK SLASLKQDNL YILIRR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

Macromolecule #9: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus)
• Molecular weight: Theoretical: 13.61232 KDa

Sequence

String:

MASFRFCLEC NNMLYPKEDK ENQRLLYSCR NCDYTELAED PKVYRHELIT NIGETAGIVD DIGQDPTLPR SDKECPECHS RDCVFFQSQ QRRKDTNMTL FYVCLNCKKT FRDESE

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #10: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, I...

• Macromolecule: Name: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III; type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 8.554064 KDa

Sequence

String:

MIIPVRCFSC GKVVGDKWDA YLRLLEEGKQ EGDALDELKL KRYCCRRMVL THVDLIEKFL RYNPLEKKDF DS

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

Macromolecule #11: RNA polymerase II subunit B12.5

• Macromolecule: Name: RNA polymerase II subunit B12.5 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 13.832896 KDa

Sequence

String:

MNAPDRFELF ILPDDVPKLK ITPDSRVPNC IIIKFEREDH TLANLLREEL ALYPDVTFVA YKVEHPLFAN FVMRLQTEEG TRPKQALER ACASIINKLK TLDHKFNEEW NIKNFSLND

UniProtKB: RNA polymerase II subunit B12.5

Macromolecule #12: RNA polymerase subunit ABC10-alpha

• Macromolecule: Name: RNA polymerase subunit ABC10-alpha / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus)
• Molecular weight: Theoretical: 7.862048 KDa

Sequence

String:

MSREGFVAPS GTDLAAAASG VAPNKHYGVK YTCGACAHNF SLNKSDPVRC KECGHRVIYK ARTKRMIQFD AR

UniProtKB: RNA polymerase subunit ABC10-alpha

Macromolecule #13: Transcription elongation factor 1 homolog

• Macromolecule: Name: Transcription elongation factor 1 homolog / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 12.606896 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPGMGKRKSS ARKPAPKIKQ KLETQFTCLF CNHDNSVVCT LDKKNSIGLL ECKKCNLSFQ APINSLSQPI DIYSDWIDAC EAVAEENAD VNGDNFIEND GADREQDDDY DDEF

UniProtKB: Transcription elongation factor 1 homolog

Macromolecule #17: Transcription elongation factor SPT4

• Macromolecule: Name: Transcription elongation factor SPT4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 12.039614 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MRERACMLCG IVLPGRVFMQ NGCPNCDSVL NLRDSDQATV NECTSSSFEG LVAVGDNEHS WVAKWLRVDR FQPGLYAVRV DGRLPSDIV AALEQYGVYY RPRDGSVID

UniProtKB: Transcription elongation factor SPT4

Macromolecule #18: Chromatin elongation factor SPT5

• Macromolecule: Name: Chromatin elongation factor SPT5 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 101.459422 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPGMSETHKN QLDKVSTVSP DGPSEAVKEH SLQSKDLSKN DGQFIVPLDR NVIEQEEHKQ VKSSAQAHNT TGDAADNEIE DGVPSEDVE FDKFKEDDYD EDDEVEEEGD IRSRKRRRHN QFLDVEAEVD DEEDDDDDDD DVELKHDFIQ DDHIQHETQN E GFIAGHVD DDRLHRKLDQ SREKIADQDA QELADEFKQR YGRSASSKYM GSASTTAPQR LLIPTVDDPG IWGVKVRLGK EK DVVRQIL KKKLAREGTK NPLEIYSAFQ RDSFKGHVYI EARKAEAIND ALKGNVNVFS NNSKFLVGIV EYKDLLRPVK SSD VKLTRG SYVRVKNGKF KGDLAQVDEV LENGLEARLK LVPRLDYGKD LSHLSTSSSV DSTKNRRKFY TSKFRPAQRL FSEA EARVH EPTIRRDRDG FVTYGGEEYY EGFLYKTFRL QNLIVNSINP TLNELSLFQS NEESTTIDLS TIADSLKETA KNLVS FQPG DNVEIINGEL NHLTGTVSSV NQSTIVSVRL HSDDDTINSE TVEIPTSDLR KIFNVGDHVR VIHGKHTDDT GLIVEV NGD KVEFISNQTK RTVIVFSNYL IKSTDSTVSI NESGRFELHD LVQVNSDLVG IVIRAQKDSF DVLCSDGKLL SLPPVSI YS KLNLNPNQQI AIDSNGVEVK VGDTVREFTG ERRQGTILHV YRNFLFLRSR EIVENQGVFV TSSNRVKTIT SKSNGTGG Q ISGPDLSRMN PSRVIPPPSI PVANQRMTGR DPTLNKTVKI RQGGYKGKIG IVKEANGDRF RVELHNPNKT IPIPCSFLL IESTHGWVPY EDFVASDRRG GNIPRHEISG HVQQPQHGRA PAWGSGGKTP AWGSGGKTPA WGSGGSGGKT PAWGSGGKTP TWGSGAKTP AWGSGSKTPA WSGLDEEDRR DF

UniProtKB: Transcription elongation factor SPT5

Macromolecule #19: Transcription elongation factor Spt6

• Macromolecule: Name: Transcription elongation factor Spt6 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 173.241625 KDa
• Recombinant expression: Organism: Spodoptera frugiperda (fall armyworm)

Sequence

String:

GPGMSAPSPS VSEEDINETR NSIDADASTV QDLLDQDAQE GSGSDNEGSN IVDSSEEDDD EDDEEEMQKV REGFIVDDDD ENDEDGIPS STKRKHRKKH KKRSAEVVEE VDEDDLQLLM ENSGAVPGQQ QNVKFKRLKR AEQDEKAQDS DSRGLNDMFS D EEGPGGVV EEGSEEEGLE DNLTTKTQRS GNLPHDEFDD FIEEDEFSDE DDEARDERLA RMRSAKAAPQ LAGLQGEQYQ EI IEIFGDG TDYQWTLDAE EEMEQPQQDQ EYDEAGEEIK GTTTSLADVF EPSELKEKML TDEDNVIRVT DLPERFQAYR KSI KNYKLS DVDYSNERDW IVEQLKLEKR DFLQHLTQAH SSVAHLEEKF EASVKKIVDF IAIESFEVPF IWNHRRDYAL HTYN DDSNN TIIVKLLNED DLWRIVQLDL DYHSIHDKKA ALSSIYKQLD LDVVDPTYEE FFGSARTLSE LQDIDDYLTF NYSSQ VKNL TAVAELSIEG NGSGEDEEQT TKSSFAEVKM KRKYSKYAIY DRIRQDAIYP VVQSIANISQ MRENLAQSKR LHQVED PIE SPMDMIADIM STEKDKTTFI SSEKAYQAVK QFFSEQLSYE PFIRKTIRTA FQSFGVINIE LTERGKLQIE PESPYFD FK YAKNRPISAL TATPDLYLRM IQAENDGLVN IKVELPMLST VVDHFYNILK SDGTSEISEK WNALRNDAWK QSLDKLIP L VQLNVKESIR RDCERVLYFQ VKNSFTKKID QAPYQPPTYA KGTIPRVLTL SFGEGNRGDA VLGVFMDDSG DVKSQIKFD EDFQSRDFSD SLTRYIKSNN INPDIIGISG FNIHTKKLFD KVNELVNEER LTIEYDNEYG YDREEDGRSD KHLIRVIYVN DETARLYQH SSKSSAEYPN RPQLAKYCIG LAKYIQSPLL EYLALDESMY SLHIHKHQNL LPREKLIDAV QTSIVDIVNL V GVDINEAV RAPYHALALP YVCGLGPRKA AGLIQSIQRI GSNLVNRAHL ITEQLTSKTV FLNMASFVYI VFDPDVERNP QG EMDLLDS TRIHPEDYSL ARKMAADALD IEDIDDDDES AMRNAIYEMV FPRSPPKDED DLTFKLDELI LDDYATELER KHQ LKKRST LQIIKEELQS RYREIRRDFH ILNEAEIFQL LTRETVDSFR KGMVIPVYVR KVESSYMSVS TQSLIAGNIQ RQDI LEPND RRDPREVYSV GQTVRACILD VDYYNFKCQL SLLRQFTENQ VAGLNVNRNP KFWDIESENR DRQEEIDKQR EESRE SRVI KHPFFHNMKS KEAEDYLAAR PVGDVVIRPS SKGSNHITIS WKVAPQLYQH IDVLEENKDD ANAIGRVLLV GKYRYH DLD ELLVEYVNNV ANKVELMVSH DKFMSDSLDY VKEWLERYSK ANGNRSHYIF TFNRKAPGWF FLLFKLNPTS EIKIWNV KA LPDGYLLANN VYPDTNSLCN GFKTLMSSRR QIKQRSNRAG GEYNNSHAGA YDNGYSNAPR Y

UniProtKB: Transcription elongation factor Spt6

Macromolecule #20: Protein that interacts with Spt6p and copurifies with Spt5p and R...

• Macromolecule: Name: Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II; type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 46.907785 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GPGMSDEESE NKKLLDQVLP EKTDQETQPE ADAALAEQEA PENEPNEPAS DDDSDSDLSD ISDVDEEKVA EGLRYLDEQT AASLSKHKA AQPSSTHKKK EPTRRRPKQQ TARETQYEPD VVEDEAARRT RLFEEKLDAA IKRKPNKRKK NDDVDLEQMQ D ELIQQLKL QMEESAIRDA NNIEQGKPAI FKLKLLPKVK DILLRANLAD SILDNNLLAS VRLWLEPLPD ASLPAYQIQK VL FDAIKSL PIKTSHLRES GLGKVMVFYQ KSKRVEPNLK RTAEKLISDW TRPIMGASDN YKDMRVRTQQ FDPAQFAESL PGR VSVRPQ EAKTLYEEAA ERRKRAAIPQ ARTAAYTIAP QVNTELLMSS ARRTLPSGVG SSLSGEDQYK RLNSRLNTMG TKRK SSAKK GGISIEGRGL PQ

UniProtKB: Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II

Macromolecule #21: Component of the Paf1p complex

• Macromolecule: Name: Component of the Paf1p complex / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus)
• Molecular weight: Theoretical: 124.979008 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKDHLIHNHH KHEHAHAEHD YKDDDDKEHL YFQGSSGSSG MSLLDQDYYL SALPEDKRAE LVTLQLTIPL KSGDEVVTID FNEDVDVSQ LCVLLESENA RPDLWLSAAK VFVSKGNIAG SQEIIRKALQ SNVILDSSAS VTSLFHNFSF WLSLMEYVST N SREDQFLE YASNSLQASN SLDSGLILNG IGNGVLYAKS RRYDEALKEF DNLLKKKSTN ILAILGKAQI LYKRQKYSQA LE LYQKALT INPLIVPDPR LGIGLCFWHL NNKQLAEQAW HNSLKVHPQN NLNTKILICL AKFDYCFNES KDDDEFTALY RES LEFLHS CLKEDAKHPL LLMVLASYYF SKEDYEKVEK LCNLVLKENS RNAAFVSASH FWLARVAYHK EDYVQAQKQF KQAE DSQNS NTLAKLGYAQ CLIARNEVGD ATIYLEKFFK ENQDSKSSEM MLLLGIIYSQ SGKSYYKAII FLEKYVAVCQ EENYP ILPE AYLVLSRVYE NKDLNVALDY LMKANDILGD KANVYVLNNL GIYHFFRNNV SQSSDFFAQS LEALNNVSPQ NKEALS ITL HYNKARVEEV SNQSEAEKLY SKLMEKCPGY TSNKIRYIYL LALKSNGNNY ADVQQLLDDF PSDLEVRSFY GWFLKRY GR KNGLKQDLES QHHKDTLINY DKHDCYALLS LGNIYATIAR EMKVTDQKQN EIKRQQYLRA AQFYHKVLSI DPKNIYAA Q GIAIIFSDKE RTGLALEIFK KVRDTVQDLG TFINLGHCFM EAKQFGKAIE SYTIALEKFS NGMDSKLLVL IGRAWYHRG FYEKSMDAYK KALEVSEQAY QLSKLPALRF NIVFIQFQIA DFVKSLPNTQ RDLTTLENAL SGLNDAIKSL LKLAELEQPP YPSEDLKAR ATMGSNTLRN QLERAIQDQQ DYEMSIQEKL RTARRKQQLD EEKRLEQEQR RLEEARKRQE AELIKRQELI K QAEEWNKM DIDAAKDNND VLSEDGGEGE KKTTKKRKRK QKVSEDIAPD LDRINEENGN TEDYDPILDN EEEENYDEKD SE NKENGQN GQNEEVYPES EGDDEDDVTN TKRQKRSKGI VDEEDSE

UniProtKB: Component of the Paf1p complex

Macromolecule #22: RNAPII-associated chromatin remodeling Paf1 complex subunit

• Macromolecule: Name: RNAPII-associated chromatin remodeling Paf1 complex subunit; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus)
• Molecular weight: Theoretical: 62.301246 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKDHLIHNHH KHEHAHAEHL YFQGSSGSSG MSDKEQDLDE DLLALAGAGS DSESNSAYSP EQEVSRKSKK IVNDSEDENN DSDGEQLLN PYPLEDKFKD EADRARLLGL SEMEREAILY ERSQEVIKLK EKHLLSLRAK QSQLEKTARE GPKSSKTNKL S ELKKQREQ KSKRQRSDEY SDEEDDYRYE EVGDNDDVEM SDNYIDEDDA VFVEPKKSSR SQELSKPATL SDINKIIFGR TA MSKYWYY PEFDDVVKGM YLRLNTGSGG NGFSPYKVVE VLGSQRIKGS AYGLNSKENN CDMYLKVAFP NQKEMVRPLF VFS DSSITH PEFDLFLREL DAEGLSVMDL RDVDYKYHQL KEMSSRSLSN DEVNSIVKMK QSLSSNTGFN TVLKKAQLQE ELEE ARDAH DHERVARIEA ELKSIGAESV VASKASSSML KIDQRNKKLN NRFIRKAEMA AVEKRKLRGT SESDPFYRLA TTARM FYKS ANLGADDSNQ PKTKAELEEE AKQEKKLEMQ KLESMVKSNY RNGGLDRIIS KIDFDFDLEL

UniProtKB: RNAPII-associated chromatin remodeling Paf1 complex subunit

Macromolecule #23: Leo1

• Macromolecule: Name: Leo1 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 52.387715 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKDHLIHNHH KHEHAHAEHL YFQGSSGSSG MSQELEQNRH SGEESPDPVL DANTSDVDDD GLDLFGDDDD EQPEAKPGQL DDSSNDAEE DEQEKAGKPE AKVTSHTKSV YDNGETLDIS LPIHPKSHIP QGKQDRWVVK LPDFLDINAE PFDPRPFEMN V KTHEDKNQ ELLDKLIAVN TVRWRYAKSE TGGIFKETNS QIIQWEDGTY SLRVGSEIFD MFTTNTDDNY LVSEHNEEGI LM TESTLSK SVKLVPASFQ STTHQKLAKA LSAKQKKESY ARSVVTKEDP EERQRRLESQ ENERYRLERR RKQAEEEEDY DSY AVSTSR SRPSRASQMY NAADEDDDDD DVAIGRTLAS RRNNGYEDDG FIIDDEEEEE AVENDSADEE SENEGDDDEE DDEE AAERL NRLKRAGASK YTNEGEQAQS EPVKSEEADN ASQRDDAPRK KRRVILDDDE EEEE

UniProtKB: RNA polymerase-associated protein LEO1

Macromolecule #24: RNAP II-associated protein

• Macromolecule: Name: RNAP II-associated protein / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 46.04598 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GSAMTSSKSR QDYIAKVRYQ NDLPAPPCPP KLLKYEIEKE APQKEFLKDS RLLSALFSKD NFRYLMNETS DGLDVNYLRI PGIIENEKS LGKLFSSYKN LAIENLHPDD RLLLVDPNKS ATLNEESPVF FLRRPQYVSD GEKINLQNFT NKRKHQDMED T NPRSQLHS VERTFDEVID PRNKNRLQSL IHPRKKIKAV KAWHFFPDTS TFDQVFHSLK FVGSASLSKD RPLNEQLGQV SE TDSTSVN ASILTSLFKP IEINPHNKWI SLYAVTDKLS AESFRKSFNS IKDDNIVNRH VIYDHIKDFD QMFRGHKKLF EDF AISFDD ISDRAFFVPI VGRLELKKKR IVPGLVDMVN RTNYAHIRMD LRNPSTQETA IRDSRREQYD PVNYSSIQEE

UniProtKB: RNAP II-associated protein

Macromolecule #25: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p,...

• Macromolecule: Name: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Komagataella phaffii (fungus) / Strain: GS115 / ATCC 20864
• Molecular weight: Theoretical: 44.760652 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MKDHLIHNHH KHEHAHAEHL YFQGSSGSSG MSEPLIALRQ AIKDKKPVGV NEGETIGNAK VLNIGEKQYS LDAPTSFVIN GKEFNLKVI YQCWVFRDSS SADYITECEK ENIDGISFVE RSELISWLKG EITSSAFIKG EKVGITEENG KNEGETKSNN K RKLSDDPL LKEIASNERV LIDHNKVLRG LKPKDFSSVA KDCELRILKE KPANAKSSDS NGRSSTSVSS SSKDARNKEP II VLSPAAS SLVRMSNVKE FLQEGKFLDP SKEPASSSNL LAIQRKSSRF KTPIKLLVVD NVEKLFTKSE YWDRVVAIVT TGK DWQFKN YKYKDPQILF QKFNGFYFKY KGDAVPASVK SWNVKVLDID RVERFSDRQV VEQFWDTVEN TLVAKRYKS

UniProtKB: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p

Macromolecule #14: DNA (198-mer)

• Macromolecule: Name: DNA (198-mer) / type: dna / ID: 14 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 61.381906 KDa

Sequence

String:

(DG)(DC)(DT)(DT)(DA)(DC)(DG)(DT)(DC)(DA) (DG)(DT)(DC)(DT)(DG)(DG)(DC)(DC)(DA)(DT) (DC)(DT)(DT)(DT)(DG)(DT)(DG)(DT)(DT) (DT)(DG)(DG)(DT)(DG)(DT)(DG)(DT)(DT)(DT) (DG) (DG)(DG)(DT)(DG)(DG)(DT)(DG)(DG) (DC)(DC)(DG)(DT)(DT)(DT)(DT)(DC)(DG)(DT) (DT)(DG) (DT)(DT)(DT)(DT)(DT)(DT)(DT) (DC)(DT)(DG)(DT)(DC)(DT)(DC)(DG)(DT)(DG) (DC)(DC)(DT) (DG)(DG)(DT)(DG)(DT)(DC) (DT)(DT)(DG)(DG)(DG)(DT)(DG)(DT)(DA)(DA) (DT)(DC)(DC)(DC) (DC)(DT)(DT)(DG)(DG) (DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA)(DA)(DC) (DG)(DC)(DG)(DG)(DG) (DG)(DG)(DA)(DC) (DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA)(DC)(DG) (DT)(DG)(DC)(DG)(DT)(DT) (DT)(DA)(DA) (DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG) (DA)(DG)(DC)(DT)(DG)(DT)(DC) (DT)(DA) (DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG) (DA)(DG)(DC)(DG)(DG)(DC)(DC)(DT) (DC) (DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA) (DT)(DT)(DC)(DT)(DG)(DA)(DT)

Macromolecule #16: DNA (198-mer)

• Macromolecule: Name: DNA (198-mer) / type: dna / ID: 16 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 60.869977 KDa

Sequence

String:

(DA)(DT)(DC)(DA)(DG)(DA)(DA)(DT)(DC)(DC) (DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG) (DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA) (DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG) (DA) (DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA) (DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA) (DA)(DA) (DC)(DG)(DC)(DA)(DC)(DG)(DT) (DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC) (DC)(DC)(DC) (DC)(DG)(DC)(DG)(DT)(DT) (DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA) (DA)(DG)(DG)(DG) (DG)(DA)(DT)(DT)(DA) (DC)(DA)(DC)(DC)(DC)(DA)(DA)(DG)(DA)(DC) (DA)(DC)(DC)(DA)(DG) (DG)(DC)(DA)(DC) (DG)(DA)(DG)(DA)(DC)(DA)(DG)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DC)(DA) (DA)(DC)(DG) (DA)(DA)(DA)(DA)(DC)(DG)(DG)(DC)(DC)(DA) (DC)(DC)(DA)(DC)(DC)(DC)(DA) (DA)(DA) (DC)(DA)(DC)(DA)(DC)(DC)(DA)(DA)(DA)(DC) (DA)(DC)(DA)(DA)(DG)(DA)(DG)(DC) (DT) (DA)(DA)(DT)(DT)(DG)(DA)(DC)(DT)(DG)(DA) (DC)(DG)(DT)(DA)(DA)(DG)(DC)

Macromolecule #15: RNA (5'-R(P*AP*AP*GP*CP*CP*UP*GP*GP*UP*GP*UP*CP*UP*UP*GP*GP*GP*UP...

• Macromolecule: Name: RNA (5'-R(P*AP*AP*GP*CP*CP*UP*GP*GP*UP*GP*UP*CP*UP*UP*GP*GP*GP*UP*G)-3'); type: rna / ID: 15 / Number of copies: 1
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 6.081562 KDa

Sequence

String:

UGCCUGGUGU CUUGGGUGU

Macromolecule #26: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 26 / Number of copies: 10 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #27: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 27 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 51.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: OTHER
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 444232
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD