EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 7180, Year 2022
掲載日	2022年11月23日
著者	Atsushi Yamagata / Yoshiko Murata / Kosuke Namba / Tohru Terada / Shuya Fukai / Mikako Shirouzu /
PubMed 要旨	Calcareous soils cover one-third of all land and cause severe growth defects in plants due to the poor water solubility of iron at high pH. Poaceae species use a unique chelation strategy, whereby ...Calcareous soils cover one-third of all land and cause severe growth defects in plants due to the poor water solubility of iron at high pH. Poaceae species use a unique chelation strategy, whereby plants secrete a high-affinity metal chelator, known as phytosiderophores (mugineic acids), and reabsorb the iron-phytosiderophore complex by the yellow stripe 1/yellow stripe 1-like (YS1/YSL) transporter for efficient uptake of iron from the soil. Here, we present three cryo-electron microscopy structures of barley YS1 (HvYS1) in the apo state, in complex with an iron-phytosiderophore complex, Fe(III)-deoxymugineic acid (Fe(III)-DMA), and in complex with the iron-bound synthetic DMA analog (Fe(III)-PDMA). The structures reveal a homodimeric assembly mediated through an anti-parallel β-sheet interaction with cholesterol hemisuccinate. Each protomer adopts an outward open conformation, and Fe(III)-DMA is bound near the extracellular space in the central cavity. Fe(III)-PDMA occupies the same binding site as Fe(III)-DMA, demonstrating that PDMA can function as a potent fertilizer in an essentially identical manner to DMA. Our results provide a structural framework for iron-phytosiderophore recognition and transport by YS1/YSL transporters, which will enable the rational design of new, high-potency fertilizers.
リンク	Nat Commun / PubMed:36424382 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 2.9 Å
構造データ	32765 7wsr EMDB-32765, PDB-7wsr: Cryo-EM structure of the barley Yellow stripe 1 transporter 手法: EM (単粒子) / 解像度: 2.9 Å 32766 7wst EMDB-32766, PDB-7wst: Cryo-EM structure of the barley Yellow stripe 1 transporter in complex with Fe(III)-DMA 手法: EM (単粒子) / 解像度: 2.7 Å 32767 7wsu EMDB-32767, PDB-7wsu: Cryo-EM structure of the barley Yellow stripe 1 transporter in complex with Fe(III)-PDMA 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-Y01: CHOLESTEROL HEMISUCCINATE / コレステリルヘミスクシナ-ト ChemComp-FE: Unknown entry ChemComp-554: (2~{S})-1-[(3~{S})-3-[[(3~{S})-3,4-bis(oxidanyl)-4-oxidanylidene-butyl]amino]-4-oxidanyl-4-oxidanylidene-butyl]azetidine-2-carboxylic acid ChemComp-5ZS: (2~{S})-1-[(3~{S})-3-[[(3~{S})-3,4-bis(oxidanyl)-4-oxidanylidene-butyl]amino]-4-oxidanyl-4-oxidanylidene-butyl]pyrrolidine-2-carboxylic acid
由来	hordeum vulgare (オオムギ)
キーワード	METAL TRANSPORT / iron-phytosiderophore transporter / deoxymugineic acid / proline 2'-deoxymugineic acid