+検索条件
-Structure paper
タイトル | Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 7180, Year 2022 |
掲載日 | 2022年11月23日 |
著者 | Atsushi Yamagata / Yoshiko Murata / Kosuke Namba / Tohru Terada / Shuya Fukai / Mikako Shirouzu / |
PubMed 要旨 | Calcareous soils cover one-third of all land and cause severe growth defects in plants due to the poor water solubility of iron at high pH. Poaceae species use a unique chelation strategy, whereby ...Calcareous soils cover one-third of all land and cause severe growth defects in plants due to the poor water solubility of iron at high pH. Poaceae species use a unique chelation strategy, whereby plants secrete a high-affinity metal chelator, known as phytosiderophores (mugineic acids), and reabsorb the iron-phytosiderophore complex by the yellow stripe 1/yellow stripe 1-like (YS1/YSL) transporter for efficient uptake of iron from the soil. Here, we present three cryo-electron microscopy structures of barley YS1 (HvYS1) in the apo state, in complex with an iron-phytosiderophore complex, Fe(III)-deoxymugineic acid (Fe(III)-DMA), and in complex with the iron-bound synthetic DMA analog (Fe(III)-PDMA). The structures reveal a homodimeric assembly mediated through an anti-parallel β-sheet interaction with cholesterol hemisuccinate. Each protomer adopts an outward open conformation, and Fe(III)-DMA is bound near the extracellular space in the central cavity. Fe(III)-PDMA occupies the same binding site as Fe(III)-DMA, demonstrating that PDMA can function as a potent fertilizer in an essentially identical manner to DMA. Our results provide a structural framework for iron-phytosiderophore recognition and transport by YS1/YSL transporters, which will enable the rational design of new, high-potency fertilizers. |
リンク | Nat Commun / PubMed:36424382 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.7 - 2.9 Å |
構造データ | EMDB-32765, PDB-7wsr: EMDB-32766, PDB-7wst: EMDB-32767, PDB-7wsu: |
化合物 | ChemComp-Y01: ChemComp-FE: ChemComp-554: ChemComp-5ZS: |
由来 |
|
キーワード | METAL TRANSPORT / iron-phytosiderophore transporter / deoxymugineic acid / proline 2'-deoxymugineic acid |