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Structure paper

TitleStructures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism.
Journal, issue, pagesNat Struct Mol Biol, Vol. 28, Issue 10, Page 799-810, Year 2021
Publish dateSep 23, 2021
AuthorsQinhui Rao / Long Han / Yue Wang / Pengxin Chai / Yin-Wei Kuo / Renbin Yang / Fangheng Hu / Yuchen Yang / Jonathon Howard / Kai Zhang /
PubMed AbstractThousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces to bend microtubule ...Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Coordination among multiple OADs is essential for generating mechanical forces to bend microtubule doublets (MTDs). Using electron microscopy, we determined high-resolution structures of Tetrahymena thermophila OAD arrays bound to MTDs in two different states. OAD preferentially binds to MTD protofilaments with a pattern resembling the native tracks for its distinct microtubule-binding domains. Upon MTD binding, free OADs are induced to adopt a stable parallel conformation, primed for array formation. Extensive tail-to-head (TTH) interactions between OADs are observed, which need to be broken for ATP turnover by the dynein motor. We propose that OADs in an array sequentially hydrolyze ATP to slide the MTDs. ATP hydrolysis in turn relaxes the TTH interfaces to effect free nucleotide cycles of downstream OADs. These findings lead to a model explaining how conformational changes in the axoneme produce coordinated action of dyneins.
External linksNat Struct Mol Biol / PubMed:34556869 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 8.0 Å
Structure data

22677

7k58

7n32

EMDB-22677, PDB-7k58:
Structure of outer-arm dyneins bound to microtubule with microtubule binding state 1(MTBS-1)
PDB-7n32: protofilaments of microtubule doublets bound to outer-arm dynein
Method: EM (single particle) / Resolution: 4.0 Å

22679

7k5b

EMDB-22679, PDB-7k5b:
Structure of outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (MTBS-2)
Method: EM (single particle) / Resolution: 4.5 Å

22840

7kek

EMDB-22840, PDB-7kek:
Structure of the free outer-arm dynein in pre-parallel state
Method: EM (single particle) / Resolution: 8.0 Å

24066

7mwg

EMDB-24066, PDB-7mwg:
16-nm repeat microtubule doublet
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-HOH:
WATER / Water

Source
  • tetrahymena thermophila (eukaryote)
KeywordsSTRUCTURAL PROTEIN / outer-arm dynein / axonemal dynein / microtubule doublet / MOTOR PROTEIN / three head / outer dynein arms / microtubule binding / OAD / axonemal / pre-paralle / protofilament of microtubule doublet

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