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TitleStructural features of nucleosomes in interphase and metaphase chromosomes.
Journal, issue, pagesMol Cell, Vol. 81, Issue 21, Page 4377-4397.e12, Year 2021
Publish dateNov 4, 2021
AuthorsYasuhiro Arimura / Rochelle M Shih / Ruby Froom / Hironori Funabiki /
PubMed AbstractStructural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we devise the template-, reference- and selection-free (TRSF) cryo-EM pipeline to simultaneously reconstruct cryo- ...Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we devise the template-, reference- and selection-free (TRSF) cryo-EM pipeline to simultaneously reconstruct cryo-EM structures of protein complexes from interphase or metaphase chromosomes. The reconstructed interphase and metaphase nucleosome structures are on average indistinguishable from canonical nucleosome structures, despite DNA sequence heterogeneity, cell-cycle-specific posttranslational modifications, and interacting proteins. Nucleosome structures determined by a decoy-classifying method and structure variability analyses reveal the nucleosome structural variations in linker DNA, histone tails, and nucleosome core particle configurations, suggesting that the opening of linker DNA, which is correlated with H2A C-terminal tail positioning, is suppressed in chromosomes. High-resolution (3.4-3.5 Å) nucleosome structures indicate DNA-sequence-independent stabilization of superhelical locations ±0-1 and ±3.5-4.5. The linker histone H1.8 preferentially binds to metaphase chromatin, from which chromatosome cryo-EM structures with H1.8 at the on-dyad position are reconstituted. This study presents the structural characteristics of nucleosomes in chromosomes.
External linksMol Cell / PubMed:34478647 / PubMed Central
MethodsEM (single particle)
Resolution3.38 - 8.1 Å
Structure data

EMDB-22790, PDB-7kbd:
Nucleosome in interphase chromosome formed in Xenopus egg extract (oligo fraction)
Method: EM (single particle) / Resolution: 3.38 Å

EMDB-22791, PDB-7kbe:
Nucleosome isolated from metaphase chromosome formed in Xenopus egg extract (oligo fraction)
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-22792, PDB-7kbf:
H1.8 bound nucleosome isolated from metaphase chromosome in Xenopus egg extract (oligo fraction)
Method: EM (single particle) / Resolution: 4.42 Å

EMDB-22793:
Nucleosome isolated from interphase chromosome formed in Xenopus egg extract (oligo fraction) (unbiased reconstruction)
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-22794:
Alpha2-macroglobulin family protein isolated from interphase chromosome formed in Xenopus egg extract
Method: EM (single particle) / Resolution: 8.07 Å

EMDB-22795:
Nucleosome isolated from metaphase chromosome formed in Xenopus egg extract (oligo fraction) (unbiased reconstruction)
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-22796:
Alpha2-macroglobulin family protein isolated from metaphase chromosome formed in Xenopus egg extract
Method: EM (single particle) / Resolution: 5.5 Å

EMDB-22797:
Nucleosome isolated from interphase chromosome formed in Xenopus egg extract (mono fraction)
Method: EM (single particle) / Resolution: 5.12 Å

EMDB-22798:
Nucleosome isolated from interphase chromosome formed in Xenopus egg extract (di fraction)
Method: EM (single particle) / Resolution: 4.74 Å

EMDB-22800:
Nucleosome isolated from metaphase chromosome formed in Xenopus egg extract (mono fraction)
Method: EM (single particle) / Resolution: 5.64 Å

EMDB-22801:
Nucleosome isolated from metaphase chromosome formed in Xenopus egg extract (di fraction)
Method: EM (single particle) / Resolution: 8.1 Å

EMDB-23819:
Nucleosome isolated from the interphase chromosome (oligo fraction, egg extract lot 2)
Method: EM (single particle) / Resolution: 3.54 Å

EMDB-23820:
Nucleosome isolated from the interphase chromosome (oligo fraction, egg extract lot 2, simultaneous structures reconstruction)
Method: EM (single particle) / Resolution: 4.42 Å

EMDB-23821:
Nucleosome isolated from the metaphase chromosome (oligo fraction, egg extract lot 2)
Method: EM (single particle) / Resolution: 3.77 Å

EMDB-23822:
Nucleosome isolated from the metaphase chromosome (oligo fraction, egg extract lot 2, simultaneous structures reconstruction)
Method: EM (single particle) / Resolution: 4.32 Å

EMDB-23823:
Closed linker DNA nucleosome reconstituted with GUB DNA
Method: EM (single particle) / Resolution: 3.77 Å

EMDB-23824:
Open linker DNA nucleosome reconstituted with GUB DNA
Method: EM (single particle) / Resolution: 4.52 Å

EMDB-23826:
Nucleosome isolated from interphase chromosome formed in Xenopus egg extract (crosslinked after MNase, egg extract lot 2)
Method: EM (single particle) / Resolution: 5.44 Å

Source
  • xenopus laevis (African clawed frog)
  • African clawed frog (African clawed frog)
  • synthetic construct (others)
KeywordsSTRUCTURAL PROTEIN/DNA / nucleosome / interphase / cell cycle / chromatin / DNA BINDING PROTEIN / STRUCTURAL PROTEIN-DNA complex / DNA BINDING PROTEIN/DNA / M phase / Xenopus egg extract / DNA BINDING PROTEIN-DNA complex / H1 / Chromatosome / Linker histone

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