Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å.
Journal, issue, pages	Nat Commun, Vol. 11, Issue 1, Page 4331, Year 2020
Publish date	Aug 28, 2020
Authors	Jonathan A Flores / Bassam G Haddad / Kimberly A Dolan / Janette B Myers / Craig C Yoshioka / Jeremy Copperman / Daniel M Zuckerman / Steve L Reichow /
PubMed Abstract	Gap junctions establish direct pathways for cells to transfer metabolic and electrical messages. The local lipid environment is known to affect the structure, stability and intercellular channel ...Gap junctions establish direct pathways for cells to transfer metabolic and electrical messages. The local lipid environment is known to affect the structure, stability and intercellular channel activity of gap junctions; however, the molecular basis for these effects remains unknown. Here, we incorporate native connexin-46/50 (Cx46/50) intercellular channels into a dual lipid nanodisc system, mimicking a native cell-to-cell junction. Structural characterization by CryoEM reveals a lipid-induced stabilization to the channel, resulting in a 3D reconstruction at 1.9 Å resolution. Together with all-atom molecular dynamics simulations, it is shown that Cx46/50 in turn imparts long-range stabilization to the dynamic local lipid environment that is specific to the extracellular lipid leaflet. In addition, ~400 water molecules are resolved in the CryoEM map, localized throughout the intercellular permeation pathway and contributing to the channel architecture. These results illustrate how the aqueous-lipid environment is integrated with the architectural stability, structure and function of gap junction communication channels.
External links	Nat Commun / PubMed:32859914 / PubMed Central
Methods	EM (single particle)
Resolution	1.9 - 2.5 Å
Structure data	22358 7jjp 7jkc EMDB-22358: Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 angstroms PDB-7jjp: Sheep Connexin-50 at 1.9 angstroms resolution by CryoEM PDB-7jkc: Sheep Connexin-46 at 1.9 angstroms resolution by CryoEM Method: EM (single particle) / Resolution: 1.94 Å 22382 7jlw 7jmd EMDB-22382, PDB-7jlw: Sheep Connexin-50 at 2.5 angstroms resolution, Lipid Class 1 PDB-7jmd: Sheep Connexin-46 at 2.5 angstroms resolution, Lipid Class 1 Method: EM (single particle) / Resolution: 2.5 Å 22390 7jm9 7jn0 EMDB-22390, PDB-7jm9: Sheep Connexin-50 at 2.5 angstroms reoslution, Lipid Class 2 PDB-7jn0: Sheep Connexin-46 at 2.5 angstroms resolution, Lipid Class 2 Method: EM (single particle) / Resolution: 2.5 Å 22391 7jmc 7jn1 EMDB-22391, PDB-7jmc: Sheep Connexin-50 at 2.5 angstroms resolution, Lipid Class 3 PDB-7jn1: Sheep Connexin-46 at 2.5 angstroms resolution, Lipid Class 3 Method: EM (single particle) / Resolution: 2.5 Å
Chemicals	ChemComp-MC3: 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE / phospholipidYM ChemComp-HOH*: WATER
Source	ovis aries (sheep) Sheep (sheep)
Keywords	MEMBRANE PROTEIN / Connexin / Gap Junction / Nanodisc / Lipid