EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures provide insight into how E. coli FF ATP synthase accommodates symmetry mismatch.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 2615, Year 2020
掲載日	2020年5月26日
著者	Meghna Sobti / James L Walshe / Di Wu / Robert Ishmukhametov / Yi C Zeng / Carol V Robinson / Richard M Berry / Alastair G Stewart /
PubMed 要旨	FF ATP synthase functions as a biological rotary generator that makes a major contribution to cellular energy production. It comprises two molecular motors coupled together by a central and a ...FF ATP synthase functions as a biological rotary generator that makes a major contribution to cellular energy production. It comprises two molecular motors coupled together by a central and a peripheral stalk. Proton flow through the F motor generates rotation of the central stalk, inducing conformational changes in the F motor that catalyzes ATP production. Here we present nine cryo-EM structures of E. coli ATP synthase to 3.1-3.4 Å resolution, in four discrete rotational sub-states, which provide a comprehensive structural model for this widely studied bacterial molecular machine. We observe torsional flexing of the entire complex and a rotational sub-step of F associated with long-range conformational changes that indicates how this flexibility accommodates the mismatch between the 3- and 10-fold symmetries of the F and F motors. We also identify density likely corresponding to lipid molecules that may contribute to the rotor/stator interaction within the F motor.
リンク	Nat Commun / PubMed:32457314 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 3.4 Å
構造データ	20167 6oqr EMDB-20167, PDB-6oqr: E. coli ATP Synthase ADP State 1a 手法: EM (単粒子) / 解像度: 3.1 Å 20168 6oqs EMDB-20168, PDB-6oqs: E. coli ATP synthase State 1b 手法: EM (単粒子) / 解像度: 3.3 Å 20169 6oqt EMDB-20169, PDB-6oqt: E. coli ATP synthase State 1c 手法: EM (単粒子) / 解像度: 3.1 Å 20170 6oqu EMDB-20170, PDB-6oqu: E. coli ATP synthase State 1d 手法: EM (単粒子) / 解像度: 3.2 Å 20171 6oqv EMDB-20171, PDB-6oqv: E. coli ATP Synthase State 2b 手法: EM (単粒子) / 解像度: 3.3 Å 20172 6oqw EMDB-20172, PDB-6oqw: E. coli ATP synthase State 3a 手法: EM (単粒子) / 解像度: 3.1 Å 20454 6pqv EMDB-20454, PDB-6pqv: E. coli ATP Synthase State 1e 手法: EM (単粒子) / 解像度: 3.3 Å 21419 6vwk EMDB-21419, PDB-6vwk: E. coli ATP Synthase ADP Sub-state 3a Fo Focussed 手法: EM (単粒子) / 解像度: 3.3 Å 21854 6wnq EMDB-21854, PDB-6wnq: E. coli ATP Synthase State 2a 手法: EM (単粒子) / 解像度: 3.4 Å 21855 6wnr EMDB-21855, PDB-6wnr: E. coli ATP synthase State 3b 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-PO4: PHOSPHATE ION / ホスファ-ト
由来	escherichia coli (大腸菌) Escherichia coli escherichia coli 2-427-07_s4_c3 (大腸菌) escherichia coli o145:nm (大腸菌) escherichia coli chi7122 (大腸菌) escherichia coli xuzhou21 (大腸菌)
キーワード	MEMBRANE PROTEIN / E coli ATP Synthase / ion channel / ATPase / F1Fo ATP synthase