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-Structure paper
| タイトル | Towards a molecular picture of the archaeal cell surface. |
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| ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 10401, Year 2024 |
| 掲載日 | 2024年11月29日 |
著者 | Matthew C Gaines / Michail N Isupov / Mathew McLaren / Clara L Mollat / Risat Ul Haque / Jake K Stephenson / Shamphavi Sivabalasarma / Cyril Hanus / Daniel Kattnig / Vicki A M Gold / Sonja Albers / Bertram Daum / ![]() |
| PubMed 要旨 | Archaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four. These ...Archaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four. These include rotary swimming propellers analogous to bacterial flagella (archaella), pili for twitching motility (Aap), adhesive fibres (threads), and filaments facilitating homologous recombination upon UV stress (UV pili). Here, we use cryo-electron microscopy to describe the structure of the S. acidocaldarius archaellum at 2.0 Å resolution, and update the structures of the thread and the Aap pilus at 2.7 Å and 2.6 Å resolution, respectively. We define features unique to archaella of the order Sulfolobales and compare their structure to those of Aap and threads in the context of the S-layer. We define distinct N-glycan patterns in the three filaments and identify a putative O-glycosylation site in the thread. Finally, we ascertain whether N-glycan truncation leads to structural changes in archaella and Aap. |
リンク | Nat Commun / PubMed:39614099 / PubMed Central |
| 手法 | EM (らせん対称) |
| 解像度 | 2.03 - 2.65 Å |
| 構造データ | EMDB-18700, PDB-8qx4: EMDB-19608, PDB-8rzl: EMDB-19960, PDB-9ets: EMDB-19961, PDB-9ett: EMDB-19990, PDB-9ev0: |
| 化合物 | ![]() ChemComp-MAN: |
| 由来 |
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キーワード | PROTEIN FIBRIL / cell surface appendage / N-glycosylation / O-glycosylation / mutagenesis |
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sulfolobus acidocaldarius (好気性・好酸性)
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