[English] 日本語
Yorodumi Papers
- Database of articles cited by EMDB/PDB/SASBDB data -

+
Search query

Keywords
Structure methods
Author
Journal
IF

-
Structure paper

TitleCrystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus.
Journal, issue, pagesScience, Vol. 326, Issue 5957, Page 1279-1283, Year 2009
Publish dateNov 27, 2009
AuthorsRajiv G Tawar / Stéphane Duquerroy / Clemens Vonrhein / Paloma F Varela / Laurence Damier-Piolle / Nathalie Castagné / Kirsty MacLellan / Hugues Bedouelle / Gérard Bricogne / David Bhella / Jean-François Eléouët / Félix A Rey /
PubMed AbstractThe respiratory syncytial virus (RSV) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. To help elucidate the replication mechanism of ...The respiratory syncytial virus (RSV) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. To help elucidate the replication mechanism of this RNA virus, we determined the three-dimensional (3D) crystal structure at 3.3 A resolution of a decameric, annular ribonucleoprotein complex of the RSV nucleoprotein (N) bound to RNA. This complex mimics one turn of the viral helical nucleocapsid complex, which serves as template for viral RNA synthesis. The RNA wraps around the protein ring, with seven nucleotides contacting each N subunit, alternating rows of four and three stacked bases that are exposed and buried within a protein groove, respectively. Combined with electron microscopy data, this structure provides a detailed model for the RSV nucleocapsid, in which the bases are accessible for readout by the viral polymerase. Furthermore, the nucleoprotein structure highlights possible key sites for drug targeting.
External linksScience / PubMed:19965480
MethodsEM (helical sym.) / X-ray diffraction
Resolution3.29 - 26.0 Å
Structure data

EMDB-1622:
Helical reconstruction of Respiratory Syncytial Virus N-RNA helices
Method: EM (helical sym.) / Resolution: 26.0 Å

PDB-2wj8:
Respiratory Syncitial Virus RiboNucleoProtein
Method: X-RAY DIFFRACTION / Resolution: 3.29 Å

Chemicals

ChemComp-BO4:
BORATE ION

ChemComp-HOH:
WATER

Source
  • Respiratory syncytial virus
  • human respiratory syncytial virus a strain long
  • escherichia coli (E. coli)
KeywordsRNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN RNA COMPLEX / NUCLEOCAPSID (N) / RIBONUCLEOPROTEIN / RESPIRATORY SYNCYTIAL VIRUS (RSV) / VIRAL NUCLEOPROTEIN / TEMPLATE-LIKE ASSEMBLY / RNA / VIRION / COMPLEX / CYTOPLASM / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex

+
About Yorodumi Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi Papers

Database of articles cited by EMDB/PDB/SASBDB data

  • Database of articles cited by EMDB, PDB, and SASBDB entries
  • Using PubMed data

Related info.:EMDB / PDB / SASBDB / Yorodumi / EMN Papers / Changes in new EM Navigator and Yorodumi

Read more