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TitleStructures of RecBCD in complex with phage-encoded inhibitor proteins reveal distinctive strategies for evasion of a bacterial immunity hub.
Journal, issue, pagesElife, Vol. 11, Year 2022
Publish dateDec 19, 2022
AuthorsMartin Wilkinson / Oliver J Wilkinson / Connie Feyerherm / Emma E Fletcher / Dale B Wigley / Mark S Dillingham /
PubMed AbstractFollowing infection of bacterial cells, bacteriophage modulate double-stranded DNA break repair pathways to protect themselves from host immunity systems and prioritise their own recombinases. Here, ...Following infection of bacterial cells, bacteriophage modulate double-stranded DNA break repair pathways to protect themselves from host immunity systems and prioritise their own recombinases. Here, we present biochemical and structural analysis of two phage proteins, gp5.9 and Abc2, which target the DNA break resection complex RecBCD. These exemplify two contrasting mechanisms for control of DNA break repair in which the RecBCD complex is either inhibited or co-opted for the benefit of the invading phage. Gp5.9 completely inhibits RecBCD by preventing it from binding to DNA. The RecBCD-gp5.9 structure shows that gp5.9 acts by substrate mimicry, binding predominantly to the RecB arm domain and competing sterically for the DNA binding site. Gp5.9 adopts a parallel coiled-coil architecture that is unprecedented for a natural DNA mimic protein. In contrast, binding of Abc2 does not substantially affect the biochemical activities of isolated RecBCD. The RecBCD-Abc2 structure shows that Abc2 binds to the Chi-recognition domains of the RecC subunit in a position that might enable it to mediate the loading of phage recombinases onto its single-stranded DNA products.
External linksElife / PubMed:36533901 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.8 Å
Structure data

15803

8b1r

EMDB-15803, PDB-8b1r:
RecBCD in complex with the phage protein gp5.9
Method: EM (single particle) / Resolution: 3.2 Å

15804

8b1t

EMDB-15804, PDB-8b1t:
RecBCD-DNA in complex with the phage protein Abc2
Method: EM (single particle) / Resolution: 3.4 Å

15805

8b1u

EMDB-15805, PDB-8b1u:
RecBCD-DNA in complex with the phage protein Abc2 and host PpiB
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

Source
  • escherichia coli (E. coli)
  • escherichia phage t7 (virus)
  • synthetic construct (others)
  • salmonella phage p22 (virus)
KeywordsDNA BINDING PROTEIN / Homologous recombination / DNA repair / phage / Helicase / Nuclease / Inhibitor / Protein complex / Enzyme / DNA mimic

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