EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational changes in mitochondrial complex I of the thermophilic eukaryote .
ジャーナル・号・ページ	Sci Adv, Vol. 8, Issue 47, Page eadc9952, Year 2022
掲載日	2022年11月25日
著者	Eike Laube / Jakob Meier-Credo / Julian D Langer / Werner Kühlbrandt /
PubMed 要旨	Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. ...Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic fungus , determined by cryoEM up to 2.4-Å resolution. We show that the complex undergoes a transition between two conformations, which we refer to as state 1 and state 2. The conformational switch is manifest in a twisting movement of the peripheral arm relative to the membrane arm, but most notably in substantial rearrangements of the Q-binding cavity and the E-channel, resulting in a continuous aqueous passage from the E-channel to subunit ND5 at the far end of the membrane arm. The conformational changes in the complex interior resemble those reported for mammalian complex I, suggesting a highly conserved, universal mechanism of coupling electron transport to proton pumping.
リンク	Sci Adv / PubMed:36427319 / PubMed Central
手法	EM (単粒子)
解像度	2.44 - 2.83 Å
構造データ	14791 7zm7 EMDB-14791, PDB-7zm7: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM) 手法: EM (単粒子) / 解像度: 2.77 Å 14792 7zm8 EMDB-14792, PDB-7zm8: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM) - membrane arm 手法: EM (単粒子) / 解像度: 2.76 Å 14794 7zmb EMDB-14794, PDB-7zmb: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2) 手法: EM (単粒子) / 解像度: 2.75 Å 14796 7zme EMDB-14796, PDB-7zme: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2) - membrane arm 手法: EM (単粒子) / 解像度: 2.83 Å 14797 7zmg EMDB-14797, PDB-7zmg: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1) 手法: EM (単粒子) / 解像度: 2.44 Å 14798 7zmh EMDB-14798, PDB-7zmh: CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1) - membrane arm 手法: EM (単粒子) / 解像度: 2.47 Å
化合物	ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH ChemComp-ZN: Unknown entry ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-HOH: WATER ChemComp-LMN: Lauryl Maltose Neopentyl Glycol / 可溶化剤*YM
由来	chaetomium thermophilum var. thermophilum dsm 1495 (菌類)
キーワード	OXIDOREDUCTASE / Proton transporter / Mitochondrial membrane protein / Complex